Results 111 to 120 of about 15,403 (255)

CRISPR/Cas9 nickase-mediated cytosine base editing in Trichoderma koningiopsis

Mycology
Base editors are regarded as reliable and precise editing tools due to their ability to efficiently and accurately modify the target genes without inducing double-strand breaks (DSBs).
Yushi Fu, Yuxia Duan, Yongpeng Yao, Yuanyuan Pan, Zhaoqing Zeng, Wenying Zhuang, Gang Liu   +6 more
doaj   +1 more source

The Role of miRNAs in Chicken Immune Regulation and Prospects for Disease‐Resistant Breeding

Animal Research and One Health, EarlyView.
A schematic workflow illustrating the screening of disease‐resistant miRNAs and the generation of miRNA‐based disease‐resistant chickens via PGC‐mediated germline genome editing. ABSTRACT MicroRNAs (miRNAs) are emerging as pivotal regulators of the immune system, playing a decisive role in shaping disease resistance in chicken.
Qiangzhou Wang, Weiran Wu, Qingqing Cai, Jiaxing Wang, Qi Gao, Muhammad Iftikhar, Yulin Bi, Hao Bai, Lihong Gu, Guobin Chang, Shihao Chen   +10 more
wiley   +1 more source

Improved Dual Base Editor Systems (iACBEs) for Simultaneous Conversion of Adenine and Cytosine in the Bacterium Escherichia coli

, 2023
Genome-editing (GE) techniques like base editing are ideal for introducing novel gain-of-function mutations and in situ protein evolution. Features of base editors (BEs) such as higher efficacy, relaxed protospacer adjacent motif (PAM), and a broader ...
Jae-Yean Kim, Dibyajyoti Pramanik, Rahul Mahadev Shelake   +2 more
core   +1 more source

DNA (cytosine‐5)‐methyltransferase 3A (Dnmt3a) mutations limit normal and autoreactive CD4+ T follicular helper responses and attenuate T cell‐driven joint inflammation

Arthritis &Rheumatology, Accepted Article.
Objective Somatic DNMT3A mutations are the most common drivers of clonal hematopoiesis in patients with rheumatoid arthritis (RA) and have been associated with seropositive disease and increased inflammatory markers. These mutations are predominantly hypomorphic or dominant‐negative, reducing DNMT3A function.
Yunbing Shen, Sanjaykumar Boddul, Zhaojun Li, Zsolt Kasza, Marlene Schülein, Bruno Raposo, Karine Chemin, Per‐Johan Jakobsson, Alexander Espinosa, Lars Klareskog, Fredrik Wermeling   +10 more
wiley   +1 more source

Designing the MyoFusion Media: A Serum‐Free Medium Optimized for Bovine Satellite Cell Differentiation

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Cultivated meat production requires efficient differentiation of muscle progenitor cells into myotubes without relying on animal‐derived serum, which poses ethical and scalability challenges. This study aimed to develop a chemically defined, serum‐free medium optimized for bovine satellite cell differentiation.
Aysenaz Tavsanli, Viktor Milkevych, Jette Feveile Young, Martin Krøyer Rasmussen   +3 more
wiley   +1 more source

A Modular Nucleoside Kinase Cascade for the Synthesis of Ribonucleoside Triphosphates

Biotechnology and Bioengineering, EarlyView.
Damm et al. report standardized enzymatic cascades for production of all canonical nucleoside triphosphates from the corresponding nucleosides, using kinase‐catalyzed phosphorylation from acetyl phosphate. Reactions afforded ∼ 100 mM product in near‐quantitative conversion.
Oliver T. Damm, Martin Pfeiffer, Johannes Zöhrer, Bernd Nidetzky   +3 more
wiley   +1 more source

Generation of Herbicide-Resistant Soybean by Base Editing

, 2023
Weeds cause the largest yield loss in soybean production. The development of herbicide-resistant soybean germplasm is of great significance for weed control and yield improvement.
Tao Wei, Zhen Xi, Pengyu Ma, Xiang You, Linjian Jiang, Ning Ning Wang   +5 more
core   +1 more source

Continuous Purification of mRNA Produced by In Vitro Transcription Using High Performance Countercurrent Membrane Purification

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Messenger ribonucleic acid (mRNA) therapeutics produced by in vitro transcription must be purified to remove residual enzymes and free nucleotides. This study examines the use of high‐performance countercurrent membrane purification (HPCMP) for the purification of mRNA therapeutics based on differences in the rate of diffusion across ...
Ziqiao Wang, Amin Javidanbardan, Ali Behboudi, Andrew L. Zydney   +3 more
wiley   +1 more source

Reprogramming tumor immune microenvironment by ultrasound‐responsive nanoplatforms for enhanced cancer immunotherapy

BMEMat, EarlyView.
Ultrasound‐responsive nanoplatforms reprogram the tumor immune microenvironment by targeting tumor cells, immune cells, and non‐immune stromal cells to enhance the efficacy of cancer immunotherapy. Abstract Cancer immunotherapy represents a significant advancement in cancer treatment by enhancing the specific recognition and elimination of cancer cells.
Shilong Zhao, Rui Qu, Zan Dai, Xiqun Jiang, Xu Zhen   +4 more
wiley   +1 more source

Status and future of recombinant adeno‐associated virus vector manufacturing

Biotechnology Progress, EarlyView.
Abstract Sixty years of adeno‐associated virus (AAV) research illustrates a trajectory marked by basic science exploration, iterative innovation, persistent challenges, a number of clinical setbacks, as well as commercial therapeutic triumphs. This continual evolution has led to recombinant AAV (rAAV) becoming a cornerstone of modern gene therapy ...
Frank Agbogbo, David Dismuke
wiley   +1 more source