Results 231 to 240 of about 15,403 (255)

Therapeutic Innovations for Monkeypox Inhibition. [PDF]

Int J Mol Sci
De N, Bhadra J, Momin MSA, Mitra K, Bhunia D, Sannigrahi A.   +5 more
europepmc   +1 more source

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Precise mutagenesis in zebrafish using cytosine base editors

Nature Protocols, 2023
Base editing is a powerful CRISPR-based technology for introducing precise substitutions into the genome. This technology greatly advances mutagenesis possibilities in vivo, particularly in zebrafish, for which the generation of precise point mutations is still challenging.
Marion Rosello, Malo Serafini, Jean-Paul Concordet, Filippo Del Bene   +3 more
openaire   +2 more sources

A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing

Nature Biotechnology, 2020
Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR-Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits.
Julian Grunewald, Ronghao Zhou, Caleb A Lareau   +2 more
exaly   +6 more sources

Dual base editor catalyzes both cytosine and adenine base conversions in human cells

Nature Biotechnology, 2020
Although base editors are useful tools for precise genome editing, current base editors can only convert either adenines or cytosines. We developed a dual adenine and cytosine base editor (A&C-BEmax) by fusing both deaminases with a Cas9 nickase to achieve C-to-T and A-to-G conversions at the same target site. Compared to single base editors, A&C-BEmax'
Biyun Zhu, Shuming Yin, Li Yang
exaly   +3 more sources

Germline Editing of Drosophila Using CRISPR-Cas9-Based Cytosine and Adenine Base Editors

The CRISPR Journal, 2023
Target-AID, BE3, and ABE7.10 base editors fused to the catalytically modified Cas9 and xCas9(3.7) were tested for germline editing of the fruit fly Drosophila melanogaster . We developed a guide RNA-expressing construct, white- 4gRNA, targeting splice ...
Nirav Thakkar, Adela Hejzlarova, Vaclav Brabec, David Dolezel   +3 more
openaire   +3 more sources

Genome Engineering Human ESCs or iPSCs with Cytosine and Adenine Base Editors

2022
The ability to engineer specific mutations in human embryonic stem cells (ECSs) or induced pluripotent stem cells (iPSCs) is extremely important in the modeling of human diseases and the study of biological processes. While CRISPR/Cas9 can robustly generate gene knockouts (KOs) and gene loci modifications in coding sequences of iPSCs, it remains ...
Giulia, Pavani, Joshua G, Klein, Deborah L, French, Paul, Gadue   +3 more
openaire   +2 more sources

Precision genome editing using cytosine and adenine base editors in mammalian cells

Nature Protocols, 2021
Genome editing has transformed the life sciences and has exciting prospects for use in treating genetic diseases. Our laboratory developed base editing to enable precise and efficient genome editing while minimizing undesired byproducts and toxicity associated with double-stranded DNA breaks.
Tony P. Huang, Gregory A. Newby, David R. Liu   +2 more
openaire   +2 more sources

Cytosine base editors go off-target

Nature Reviews Genetics, 2019
Two studies in Science show that cytosine base editors, but not adenine base editors or CRISPR–Cas9, induce notable off-target single-nucleotide variants in rice and in mouse embryos.
openaire   +2 more sources