Results 211 to 220 of about 15,248 (235)

Genome Engineering Human ESCs or iPSCs with Cytosine and Adenine Base Editors

2022
The ability to engineer specific mutations in human embryonic stem cells (ECSs) or induced pluripotent stem cells (iPSCs) is extremely important in the modeling of human diseases and the study of biological processes. While CRISPR/Cas9 can robustly generate gene knockouts (KOs) and gene loci modifications in coding sequences of iPSCs, it remains ...
Giulia, Pavani, Joshua G, Klein, Deborah L, French, Paul, Gadue   +3 more
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Precision genome editing using cytosine and adenine base editors in mammalian cells

Nature Protocols, 2021
Genome editing has transformed the life sciences and has exciting prospects for use in treating genetic diseases. Our laboratory developed base editing to enable precise and efficient genome editing while minimizing undesired byproducts and toxicity associated with double-stranded DNA breaks.
Tony P. Huang, Gregory A. Newby, David R. Liu   +2 more
openaire   +2 more sources

Cytosine base editors go off-target

Nature Reviews Genetics, 2019
Two studies in Science show that cytosine base editors, but not adenine base editors or CRISPR–Cas9, induce notable off-target single-nucleotide variants in rice and in mouse embryos.
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Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors

Nature Biotechnology, 2020
Targeted saturation mutagenesis of crop genes could be applied to produce genetic variants with improved agronomic performance. However, tools for directed evolution of plant genes, such as error-prone PCR or DNA shuffling, are limited1. We engineered five saturated targeted endogenous mutagenesis editors (STEMEs) that can generate de novo mutations ...
Chao Li, Rui Zhang, Xiangbing Meng, Sha Chen, Yuan Zong, Chunju Lu, Jin-Long Qiu, Yu-Hang Chen, Jiayang Li, Caixia Gao   +9 more
openaire   +2 more sources

Computational design of a high-precision mitochondrial DNA cytosine base editor

Nature Structural & Molecular Biology
Bystander editing remains a major limitation of current base editors, hindering their precision and therapeutic potential. Here, we present a de novo protein design strategy that creates a structurally rigid interface between a DNA-binding TALE domain and a cytosine deaminase, forming a unified editing module termed TALE-oriented deaminase (TOD).
Li Mi, Yu-Xuan Li, Xinchen Lv, Zi-Li Wan, Xu Liu, Kairan Zhang, Huican Li, Yue Yao, Leping Zhang, Zhe Xu, Xingyu Zhuang, Kunqian Ji, Min Jiang, Yangming Wang, Peilong Lu   +14 more
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Cytosine base editor-mediated high-efficiency myostatin editing in Hu sheep

Functional & Integrative Genomics
The cytosine base editor (CBE) enables precise C-to-T substitution without inducing DNA double-strand breaks, which offering a promising tool for editing livestock genomes to enhance economically valuable traits. In this study, using Hu sheep, characterized by high reproductive performance but suboptimal meat production as the research subject, two CBE-
Y, Wang, W J, Liu, C H, Meng, H L, Wang, Z K, Cui, J, Zhang, J L, Zhang, Y, Qian, Y X, Li, S X, Cao   +9 more
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An SpG-Cas9-based cytosine base editor expands the scope of genome editing in carrot plants

Plant Cell Reports
SpG Cas9 significantly expands the genome editing scope in carrot with NGN PAM recognition.
Rajesh Yarra, Patrick J. Krysan
openaire   +2 more sources

New cytosine base editor for plant genome editing

Science China Life Sciences, 2018
Zhenxiang, Li, Xiangyu, Xiong, Jian-Feng, Li   +2 more
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Screening an Effective Dual-Adeno-Associated Virus Split-Cytosine Base Editor System for C-to-T Conversion In Vivo

Human Gene Therapy, 2023
Junjiu Huang
exaly  

CHLOROPLAST CYTOSINE BASE EDITORS AND MITOCHONDRIA CYTOSINE BASE EDITORS IN PLANTS

2022
YANG BING, CHAR SINIAN, LI RIQING
openaire   +2 more sources