Results 251 to 260 of about 25,918 (263)

Computational design of a high-precision mitochondrial DNA cytosine base editor

Nature Structural & Molecular Biology
Bystander editing remains a major limitation of current base editors, hindering their precision and therapeutic potential. Here, we present a de novo protein design strategy that creates a structurally rigid interface between a DNA-binding TALE domain and a cytosine deaminase, forming a unified editing module termed TALE-oriented deaminase (TOD).
Li Mi, Yu-Xuan Li, Xinchen Lv, Zi-Li Wan, Xu Liu, Kairan Zhang, Huican Li, Yue Yao, Leping Zhang, Zhe Xu, Xingyu Zhuang, Kunqian Ji, Min Jiang, Yangming Wang, Peilong Lu   +14 more
openaire   +2 more sources

Cytosine base editor-mediated high-efficiency myostatin editing in Hu sheep

Functional & Integrative Genomics
The cytosine base editor (CBE) enables precise C-to-T substitution without inducing DNA double-strand breaks, which offering a promising tool for editing livestock genomes to enhance economically valuable traits. In this study, using Hu sheep, characterized by high reproductive performance but suboptimal meat production as the research subject, two CBE-
Y, Wang, W J, Liu, C H, Meng, H L, Wang, Z K, Cui, J, Zhang, J L, Zhang, Y, Qian, Y X, Li, S X, Cao   +9 more
openaire   +2 more sources

Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice

Science, 2019
Spotting off-targets from gene editing Unintended genomic modifications limit the potential therapeutic use of gene-editing tools. Available methods to find off-targets generally do not work in vivo or detect single-nucleotide changes.
Shuai Jin, Yuan Zong, Qiang Gao, Zixu Zhu, Yanpeng Wang, Peng Qin, Chengzhi Liang, Daowen Wang, Jin-Long Qiu, Feng Zhang, Caixia Gao   +10 more
openaire   +2 more sources

An SpG-Cas9-based cytosine base editor expands the scope of genome editing in carrot plants

Plant Cell Reports
SpG Cas9 significantly expands the genome editing scope in carrot with NGN PAM recognition.
Rajesh Yarra, Patrick J. Krysan
openaire   +2 more sources

New cytosine base editor for plant genome editing

Science China Life Sciences, 2018
Zhenxiang, Li, Xiangyu, Xiong, Jian-Feng, Li   +2 more
openaire   +2 more sources

Artificial evolution of OsEPSPS through an improved dual cytosine and adenine base editor generated a novel allele conferring rice glyphosate tolerance

Journal of Integrative Plant Biology, 2023
Shaoya Li, Yubing He, Lanqin Xia
exaly  

Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity

Nature Biotechnology, 2022
Alvin Hsu, Nicholas Krasnow, Amber N McElroy   +2 more
exaly  

Screening an Effective Dual-Adeno-Associated Virus Split-Cytosine Base Editor System for C-to-T Conversion In Vivo

Human Gene Therapy, 2023
Junjiu Huang
exaly  

Rapid creation of CENH3‐mediated haploid induction lines using a cytosine base editor (CBE)

Plant Biology, 2023
Song Wang
exaly  

Cytosine base editing in cyanobacteria by repressing archaic Type IV uracil‐DNA glycosylase

Plant Journal, 2023
Han Min Woo
exaly