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Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors
Nature Biotechnology, 2020Targeted saturation mutagenesis of crop genes could be applied to produce genetic variants with improved agronomic performance. However, tools for directed evolution of plant genes, such as error-prone PCR or DNA shuffling, are limited1. We engineered five saturated targeted endogenous mutagenesis editors (STEMEs) that can generate de novo mutations ...
Chao Li +9 more
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Cas9‐orthologue‐mediated cytosine and adenine base editors recognizing NNAAAA PAM sequences
Biotechnology Journal, 2023AbstractCRISPR/Cas9 system has been applied as an effective genome‐targeting technology. By fusing deaminases with Cas9 nickase (nCas9), various cytosine and adenine base editors (CBEs and ABEs) have been successfully developed that can efficiently induce nucleotide conversions and install pathogenic single nucleotide variants (SNVs) in cultured cells ...
Min Li +8 more
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Computational design of a high-precision mitochondrial DNA cytosine base editor
Nature Structural & Molecular BiologyBystander editing remains a major limitation of current base editors, hindering their precision and therapeutic potential. Here, we present a de novo protein design strategy that creates a structurally rigid interface between a DNA-binding TALE domain and a cytosine deaminase, forming a unified editing module termed TALE-oriented deaminase (TOD).
Li Mi +14 more
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Cytosine base editor-mediated high-efficiency myostatin editing in Hu sheep
Functional & Integrative GenomicsThe cytosine base editor (CBE) enables precise C-to-T substitution without inducing DNA double-strand breaks, which offering a promising tool for editing livestock genomes to enhance economically valuable traits. In this study, using Hu sheep, characterized by high reproductive performance but suboptimal meat production as the research subject, two CBE-
Y, Wang +9 more
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Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice
Science, 2019Spotting off-targets from gene editing Unintended genomic modifications limit the potential therapeutic use of gene-editing tools. Available methods to find off-targets generally do not work in vivo or detect single-nucleotide changes.
Shuai Jin +10 more
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An SpG-Cas9-based cytosine base editor expands the scope of genome editing in carrot plants
Plant Cell ReportsSpG Cas9 significantly expands the genome editing scope in carrot with NGN PAM recognition.
Rajesh Yarra, Patrick J. Krysan
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New cytosine base editor for plant genome editing
Science China Life Sciences, 2018Zhenxiang, Li +2 more
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