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Plant Biology, 2007 Transcriptional gene silencing (TCS) is often associated with an increased level of cytosine methylation in the affected promoters. The effect of methylation of the cauliflower mosaic virus (CaMV) 35S promoter sequence on its binding to factors present ...
Akira Kanazawa, Roger P Hellens
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Cytosine Methylation and DNA Repair
2006Cytosine methylation is a common form of post-replicative DNA modification seen in both bacteria and eukaryotes. Modified cytosines have long been known to act as hotspots for mutations due to the high rate of spontaneous deamination of this base to thymine, resulting in a G/T mismatch.
C P, Walsh, G L, Xu
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Genome, 1996
A new method was developed to detect DNA methylation in the Citrus genome using random amplification coupled with restriction enzyme digestion. Genomic DNA from Citrus grandis (L.) Osb., Poncirus trifoliata (L.) Raf., and their F1hybrid was amplified using 7 individual 10-mer random primers. Prior to amplification the DNA templates were digested with 2
Q, Cai, C L, Guy, G A, Moore
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A new method was developed to detect DNA methylation in the Citrus genome using random amplification coupled with restriction enzyme digestion. Genomic DNA from Citrus grandis (L.) Osb., Poncirus trifoliata (L.) Raf., and their F1hybrid was amplified using 7 individual 10-mer random primers. Prior to amplification the DNA templates were digested with 2
Q, Cai, C L, Guy, G A, Moore
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How to Distinguish Methyl-Cytosine from Cytosine with High Fidelity
Journal of Molecular Biology, 2012Methylation of cytosines in the DNA is central to the epigenetic code. The patterns along the DNA formed by these chemical marks instruct the cell which proteins to express and their faithful maintenance after replication are vital to the organism's life.
Caterina, Bianchi, Ronen, Zangi
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Methyl-Cytosine-Based Immunoprecipitation for DNA Methylation Analysis
Cold Spring Harbor Protocols, 2016In mammalian cells, DNA methylation at the 5-position of cytosine leads to recruitment of proteins that selectively recognize and bind 5-methylcytosine (5mC). Taking advantage of the structural identity of 5mC, various affinity purification-based protocols have been developed to enrich for either DNA that is modified by 5mC or proteins that recognize ...
Paul M, Lizardi +2 more
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Determination of 5‐methyl‐cytosine and cytosine in tumor DNA of cancer patients
ELECTROPHORESIS, 2005AbstractThe determination of the relative methylation in DNA tumor samples, in order to evaluate the activity of some anti‐cancer drugs, has become a very important issue in the clinical field. Capillary electrophoresis (CE) applications in this area have been done previously but no good separation for model samples or tumor samples has been reported ...
Karina, Sandoval Guerrero +3 more
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Inheritance of Cytosine Methylation.
Journal of cellular physiology, 2017There are numerous examples of parental transgenerational inheritance that is epigenetic. The informational molecules include RNA, chromatin modifications, and cytosine methylation. With advances in DNA sequencing technologies, the molecular and epigenetic mechanisms mediating these effects are now starting to be uncovered.
Desiree, Tillo +2 more
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Biochemistry, 1993
Previous spectroscopic studies demonstrated that the oligodeoxynucleotide d(CGC G3 GCG) undergoes a reversible cation-dependent transition between Watson-Crick (WC) hairpin and parallel-stranded "G-DNA" quadruplex structures [Hardin, C.C., Watson, T., Corregan, M., & Bailey, C. (1992) Biochemistry 31, 833-841].
C C, Hardin +3 more
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Previous spectroscopic studies demonstrated that the oligodeoxynucleotide d(CGC G3 GCG) undergoes a reversible cation-dependent transition between Watson-Crick (WC) hairpin and parallel-stranded "G-DNA" quadruplex structures [Hardin, C.C., Watson, T., Corregan, M., & Bailey, C. (1992) Biochemistry 31, 833-841].
C C, Hardin +3 more
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Cytosine methylation in gene-silencing mechanisms
Current Opinion in Cell Biology, 1991Cytosine methylation is associated with gene-silencing mechanisms in a number of eukaryotic organisms. Recent studies directed at the involvement of methylation in promoter inactivation, X-chromosome and duplicate sequence inactivation and in chromatin structure changes, are presented.
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Cytosine methylation mediates sexual conflict
Trends in Genetics, 2003Transposons are infectious agents in sexual populations and can go to fixation even if they reduce the fitness of the host. Sexual hosts are therefore under selective pressure to evolve defensive functions that relieve the fitness penalty imposed by transposons.
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