Chasing Particularities of Guanine- and Cytosine-Rich DNA Strands
Molecules, 2020 By substitution of natural nucleotides by their abasic analogs (i.e., 1′,2′-dideoxyribose phosphate residue) at critically chosen positions within 27-bp DNA constructs originating from the first intron of N-myc gene, we hindered hybridization
Marko Trajkovski, Janez Plavec
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Frontiers in Molecular Biosciences, 2021 Short, structured fragments of non-coding mRNA may act as molecular switches upon binding specific ligands, regulating the translation of proteins encoded downstream this mRNA sequence.
Piotr Chyży +9 more
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Modified Nucleotides for Discrimination between Cytosine and the Epigenetic Marker 5‐Methylcytosine [PDF]
Angewandte Chemie International Edition, 2016 Abstract5‐Methyl‐2′‐deoxycytosine, the most common epigenetic marker of DNA in eukaryotic cells, plays a key role in gene regulation and affects various cellular processes such as development and carcinogenesis. Therefore, the detection of 5mC can serve as an important biomarker for diagnostics.
von Watzdorf, Janina +2 more
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Statistical analysis of readthrough levels for nonsense mutations in mammalian cells reveals a major determinant of response to gentamicin. [PDF]
PLoS Genetics, 2012 The efficiency of translation termination depends on the nature of the stop codon and the surrounding nucleotides. Some molecules, such as aminoglycoside antibiotics (gentamicin), decrease termination efficiency and are currently being evaluated for ...
Célia Floquet +3 more
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Base preferences in non-templated nucleotide incorporation by MMLV-derived reverse transcriptases. [PDF]
PLoS ONE, 2013 Reverse transcriptases derived from Moloney Murine Leukemia Virus (MMLV) have an intrinsic terminal transferase activity, which causes the addition of a few non-templated nucleotides at the 3' end of cDNA, with a preference for cytosine.
Pawel Zajac +4 more
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Genome Biology, 2020 We present a base editing system, in which base editors are attached to different sites of sgRNA scaffold (sgBE). Each independent sgBE has its own specific editing pattern for a given target site.
Yanhong Wang +9 more
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Reassessment of Viroid RNA Cytosine Methylation Status at the Single Nucleotide Level [PDF]
Viruses, 2019 Composed of a few hundreds of nucleotides, viroids are infectious, circular, non-protein coding RNAs able to usurp plant cellular enzymes and molecular machineries to replicate and move in their hosts. Several secondary and tertiary RNA structural motifs have been implicated in the viroid infectious cycle, but whether modified nucleotides, such as 5C ...
Francesco Di Serio +3 more
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Interactions between the R2R3-MYB transcription factor, AtMYB61, and target DNA binding sites. [PDF]
PLoS ONE, 2013 Despite the prominent roles played by R2R3-MYB transcription factors in the regulation of plant gene expression, little is known about the details of how these proteins interact with their DNA targets.
Michael B Prouse, Malcolm M Campbell
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Enzymatic Extraction and Composition Analysis of 5′-Nucleotides from Shiitake Mushroom Stipe(香菇柄中5′-核苷酸的酶法提取及组成分析) [PDF]
Shipin kexue jishu xuebao, 2018 To improve the yield of 5′-nucleotides and increase the economic value of shiitake mushroom stipe, 5′-nucleotides were extracted from shiitake mushroom stipe by cellulase.
LI Shunfeng(李顺峰) +6 more
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Surveying the repair of ancient DNA from bones via high-throughput sequencing
BioTechniques, 2015 DNA damage in the form of abasic sites, chemically altered nucleotides, and strand fragmentation is the foremost limitation in obtaining genetic information from many ancient samples. Upon cell death, DNA continues to endure various chemical attacks such
Nathalie Mouttham +4 more
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