Results 101 to 110 of about 1,598 (175)

D-allulose 3-epimerase for low-calorie D-allulose synthesis: microbial production, characterization, and applications

Critical Reviews in Biotechnology
D-allulose, an epimer of D-fructose at C-3 position, is a low-calorie rare sugar with favorable physiochemical properties and special physiological functions, which displays promising perspectives in the food and pharmaceutical industries. Currently, D-allulose is extremely sparse in nature and is predominantly biosynthesized through the isomerization ...
Caiming Li   +2 more
exaly   +4 more sources

Properties of d-allulose 3-epimerase mined from Novibacillus thermophilus and its application to synthesis of d-allulose

Enzyme and Microbial Technology, 2021
Abstract • Allulose is an epimer of d -fructose with multiple nutritional functions, which can be biologically synthesized from d -fructose employing d -allulose 3-epimerase (DAE). In this study, NCBI database mining approach was applied to screen novel DAEs, and Novibacillus thermophilus DAE (NtDAE) exhibiting a high activity of 273.9 U/(g ...
Dong-Xu Jia   +2 more
exaly   +3 more sources

Directional immobilization of D-allulose 3-epimerase using SpyTag/SpyCatcher strategy as a robust biocatalyst for synthesizing D-allulose

Food Chemistry, 2023
D-Allulose, as low-calorie rare sugar, possessed several notable biological activities and was biosynthesized by D-allulose 3-epimerase (DAEase). Here, CcDAE from Clostridium cellulolyticum was successfully immobilization via covalent attachment (RI-CcDAE), and Resin-SpyCatcher/SpyTag-CcDAE modular (DI-CcDAE).
Fuping Lu, Hui-Min Qin
exaly   +3 more sources

Semi‐rational engineering of D‐allulose 3‐epimerase for simultaneously improving the catalytic activity and thermostability based on D‐allulose biosensor

Biotechnology Journal
AbstractBackgroundD‐Allulose is one of the most well‐known rare sugars widely used in food, cosmetics, and pharmaceutical industries. The most popular method for D‐allulose production is the conversion from D‐fructose catalyzed by D‐allulose 3‐epimerase (DAEase).
Zijie Li   +6 more
openaire   +3 more sources

Tailored magnetic silica-immobilized D-allulose 3-epimerase with enhanced stability and recyclability for efficient D-allulose production

International Journal of Biological Macromolecules
D-allulose, a low-calorie functional sweetener, is produced by the enzymatic conversion of d-fructose via D-allulose 3-epimerase (DAE) and holds significant market potential, particularly for individuals with obesity and diabetes. However, the limited reusability and stability of DAE have restricted its industrial application.
Ziqun, Xiao   +4 more
openaire   +3 more sources

Designable immobilization of D-allulose 3-epimerase on bimetallic organic frameworks based on metal ion compatibility for enhanced D-allulose production

International Journal of Biological Macromolecules
D-allulose, a low-calorie rare sugar catalyzed by D-allulose 3-epimerase (DAE), is highly sought after for its potential health benefits. However, poor reusability and stability of DAE limited its popularization in industrial applications. Although metal-organic frameworks (MOFs) offer a promising enzyme platform for enzyme immobilization, developing ...
Huayang, Tang   +4 more
openaire   +3 more sources

Engineering Escherichia coli for D-allulose biosynthesis from glycerol

Journal of Biotechnology
D-allulose, a naturally occurring monosaccharide, is present in small quantities in nature. It is considered a valuable low-calorie sweetener due to its low absorption in the digestive tract and zero energy for growth. Most of the recent efforts to produce D-allulose have focused on in vitro enzyme catalysis. However, microbial fermentation is emerging
Qiang, Guo   +5 more
openaire   +3 more sources

Characterization of a Recombinant d-Allulose 3-epimerase from Thermoclostridium caenicola with Potential Application in d-Allulose Production

Molecular Biotechnology, 2021
In recent years, with the increasing public health awareness, low-calorie rare sugars have received more attention on a global scale. D-Allulose, the C-3 epimer of D-fructose, is a representative rare sugar. It displays high sweetness and excellent physiological functions, but only provides a caloric value of 0.4 kcal/g. D-Allulose 3-epimerase (DAEase)
Jiajun Chen   +6 more
openaire   +2 more sources

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