Results 121 to 130 of about 408 (142)
Some of the next articles are maybe not open access.
[Construction and immobilization of recombinant Bacillus subtilis with D-allulose 3-epimerase].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2022D-allulose-3-epimerase (DPEase) is the key enzyme for isomerization of D-fructose to D-allulose. In order to improve its thermal stability, short amphiphilic peptides (SAP) were fused to the N-terminal of DPEase. SDS-PAGE analysis showed that the heterologously expressed DPEase folded correctly in Bacillus subtilis, and the protein size was 33 kDa ...
Yuxia, Wei +6 more
openaire +1 more source
Structural and Functional Features of Ketoso-3-Epimerases and Their Use in Production of D-Allulose
Биоорганическая химия, 2023Rare sugars attract more and more attention as safe, low-calorie sweeteners and functional compounds in the food, pharmaceutical and medical industries. The potential of the rare sugar D-allulose has been proven in a large number of theoretical and applied works but the high cost of its production is a limitation factor for its large-scall production ...
N. S. Ivanova +2 more
openaire +1 more source
Enzyme and Microbial Technology, 2022
D-Allulose 3-epimerase (DAE) is promising to be used for the production of the rare sugar D-allulose in industry. However, the poor thermostability and low catalytic efficiency limited its large-scale industrial applications. A dual-enzyme screening method was developed to measure the activity of the D-allulose 3-epimerase from Clostridium ...
Yun, Feng +6 more
openaire +2 more sources
D-Allulose 3-epimerase (DAE) is promising to be used for the production of the rare sugar D-allulose in industry. However, the poor thermostability and low catalytic efficiency limited its large-scale industrial applications. A dual-enzyme screening method was developed to measure the activity of the D-allulose 3-epimerase from Clostridium ...
Yun, Feng +6 more
openaire +2 more sources
Enzyme and Microbial Technology, 2021
The rare sugar d-allulose is an attractive sucrose substitute due to its sweetness and ultra-low caloric value. It can be produced from D-fructose using d-allulose 3-epimerase (DAE) as the biocatalyst. However, most of the reported DAEs show low catalytic efficiency and poor thermostability, which limited their further use in food industrial.
Zhangliang Zhu +6 more
openaire +2 more sources
The rare sugar d-allulose is an attractive sucrose substitute due to its sweetness and ultra-low caloric value. It can be produced from D-fructose using d-allulose 3-epimerase (DAE) as the biocatalyst. However, most of the reported DAEs show low catalytic efficiency and poor thermostability, which limited their further use in food industrial.
Zhangliang Zhu +6 more
openaire +2 more sources
Process Biochemistry, 2020
Abstract Inulin has been widely used as a cheap bioresource for producing many valuable products by enzymatic hydrolysis or microbial fermentation, such as high-fructose syrup and fructooligosaccharides. In this work, a one-pot two-enzyme reaction system was developed to produce d -allulose from inulin using A. piperis exoinulinase and Dorea sp. d -
Wen Li +5 more
openaire +1 more source
Abstract Inulin has been widely used as a cheap bioresource for producing many valuable products by enzymatic hydrolysis or microbial fermentation, such as high-fructose syrup and fructooligosaccharides. In this work, a one-pot two-enzyme reaction system was developed to produce d -allulose from inulin using A. piperis exoinulinase and Dorea sp. d -
Wen Li +5 more
openaire +1 more source
International Journal of Biological Macromolecules, 2021
Currently, low sugar and low energy have become an important trend in the food industries. Therefore, the bioconversion of the functional low-calorie rare sugars attracts more and more attention. l-Ribulose 3-epimerase (LREase) belongs to the ketose 3-epimerase (KEase) family, which could not only efficiently catalyze the reversible C-3 epimerization ...
Ding, Chen +5 more
openaire +2 more sources
Currently, low sugar and low energy have become an important trend in the food industries. Therefore, the bioconversion of the functional low-calorie rare sugars attracts more and more attention. l-Ribulose 3-epimerase (LREase) belongs to the ketose 3-epimerase (KEase) family, which could not only efficiently catalyze the reversible C-3 epimerization ...
Ding, Chen +5 more
openaire +2 more sources
Journal of the Science of Food and Agriculture, 2022
AbstractBACKGROUNDRare sugars have become promising ‘sugar alternatives’ because of their low calories and unique physiological functions. Among the family of rare sugars, d‐allulose is one of the sugars attracting interest. Ketose 3‐epimerases (KEase), including d‐tagatose 3‐epimerase (DTEase) and d‐allulose 3‐epimerase (DAEase), are mainly used for d‐
Zijie Li +6 more
openaire +2 more sources
AbstractBACKGROUNDRare sugars have become promising ‘sugar alternatives’ because of their low calories and unique physiological functions. Among the family of rare sugars, d‐allulose is one of the sugars attracting interest. Ketose 3‐epimerases (KEase), including d‐tagatose 3‐epimerase (DTEase) and d‐allulose 3‐epimerase (DAEase), are mainly used for d‐
Zijie Li +6 more
openaire +2 more sources
International Journal of Biological Macromolecules
D-allulose, a low-calorie rare sugar catalyzed by D-allulose 3-epimerase (DAE), is highly sought after for its potential health benefits. However, poor reusability and stability of DAE limited its popularization in industrial applications. Although metal-organic frameworks (MOFs) offer a promising enzyme platform for enzyme immobilization, developing ...
Huayang, Tang +4 more
openaire +2 more sources
D-allulose, a low-calorie rare sugar catalyzed by D-allulose 3-epimerase (DAE), is highly sought after for its potential health benefits. However, poor reusability and stability of DAE limited its popularization in industrial applications. Although metal-organic frameworks (MOFs) offer a promising enzyme platform for enzyme immobilization, developing ...
Huayang, Tang +4 more
openaire +2 more sources
Biotechnology Journal
AbstractBackgroundD‐Allulose is one of the most well‐known rare sugars widely used in food, cosmetics, and pharmaceutical industries. The most popular method for D‐allulose production is the conversion from D‐fructose catalyzed by D‐allulose 3‐epimerase (DAEase).
Zijie Li +6 more
openaire +2 more sources
AbstractBackgroundD‐Allulose is one of the most well‐known rare sugars widely used in food, cosmetics, and pharmaceutical industries. The most popular method for D‐allulose production is the conversion from D‐fructose catalyzed by D‐allulose 3‐epimerase (DAEase).
Zijie Li +6 more
openaire +2 more sources
Journal of Bioscience and Bioengineering, 2017
An enzyme that catalyzes C-3 epimerization between d-fructose and d-allulose was found in Arthrobacter globiformis strain M30. Arthrobacter species have long been used in the food industry and are well-known for their high degree of safety. The enzyme was purified by ion exchange and hydrophobic interaction chromatographies and characterized as a d ...
Akihide, Yoshihara +8 more
openaire +2 more sources
An enzyme that catalyzes C-3 epimerization between d-fructose and d-allulose was found in Arthrobacter globiformis strain M30. Arthrobacter species have long been used in the food industry and are well-known for their high degree of safety. The enzyme was purified by ion exchange and hydrophobic interaction chromatographies and characterized as a d ...
Akihide, Yoshihara +8 more
openaire +2 more sources