Results 51 to 60 of about 408 (142)
D-Allulose is a promising low-calorie rare sugar with significant health benefits. However, its industrial production is hindered by the low catalytic efficiency (≤33% conversion) and unfavorable equilibrium of the key enzyme, D-allulose 3-epimerase (DAE)
Yutong Lu +4 more
doaj +1 more source
Sucrose phosphorylase and related enzymes in glycoside hydrolase family 13 : discovery, application and engineering [PDF]
Sucrose phosphorylases are carbohydrate-active enzymes with outstanding potential for the biocatalytic conversion of common table sugar into products with attractive properties.
Desmet, Tom, Franceus, Jorick
core +2 more sources
The formation of isopeptide bonds in the SpyTag/SpyCatcher system and its application in the food field ABSTRACT The SpyTag/SpyCatcher system is a modular protein assembly tool based on isopeptide bond‐mediated covalent conjugation, distinguished by mild reaction conditions, rapid ligation rate, and independence from exogenous enzymes or chemical ...
Meng‐meng Cai +6 more
wiley +1 more source
Update: Enzymatische Synthese für industrielle Anwendungen
Die Biokatalyse hat sich zu einer nachhaltigen und wettbewerbsfähigen Alternative zur herkömmlichen chemischen Synthese entwickelt und ermöglicht die enzymbasierte Herstellung nicht nur von chemischen Grundstoffen, sondern auch von (nicht natürlichen) Aminosäuren, (seltenen) Zuckern und synthetischen Nukleotiden.
Thomas Bayer +4 more
wiley +1 more source
Abstract The qualified presumption of safety (QPS) procedure was developed to provide a harmonised generic pre‐evaluation to support safety risk assessments of biological agents performed by EFSA's Scientific Panels. The taxonomic identity, body of knowledge, safety concerns and antimicrobial resistance were assessed.
EFSA Panel on Biological Hazards (BIOHAZ) +26 more
wiley +1 more source
Construction and Optimization of Biosynthetic Pathway for the Production of D-Allulose from Glucose in Escherichia coli [PDF]
A pathway in Escherichia coli BL21 (DE3) was constructed to produce D-allulose from D-glucose via a phosphorylation-dephosphorylation strategy. The genes related to competitive pathways were deleted using the CRISPR/Cas9 system, and allulose-6-phosphate
JIANG Yawen, ZHANG Cangping, YANG Shaoqing, JIANG Zhengqiang, LI Yanxiao, YAN Qiaojuan
core +1 more source
Learning from Protein Engineering by Deconvolution of Multi‐Mutational Variants
First derivative of a typical experimental enzyme fitness pathway landscape mapped on the basis of ΔG values, in this case of 24 trajectories in the deconvolution of a 4‐mutational variant. Green areas indicate negative slopes and define trajectories involved in energetically favored stages, while red areas indicate positive slopes representing ...
Frank Hollmann +2 more
wiley +1 more source
Biocatalysis in packed-bed reactors: immobilization as an enabling technology [PDF]
Biocatalysis and flow chemistry are two complementary technologies that can be used to produce chemicals in a more efficient, sustainable, and safe manner. Enzyme immobilization is key to enabling their combination.
Fernández Regueiro, Cristina Lía +2 more
core +3 more sources
Preparation of Catalyst ZIF-67 and Optimize on the Synthesis of D-Allulose [PDF]
To reduce the manufacturing cost of D-allulose, a recyclable and reusable inorganic catalyst was developed for D-allulose production, and process using this catalyst to produce D-allulose also was optimized in this article. Zeolitic imidazolate framework-
Changhe DING +6 more
core +1 more source

