Results 1 to 10 of about 1,259 (144)

Safety evaluation of the food enzyme d‐psicose 3‐epimerase from the genetically modified Corynebacterium glutamicum strain FIS002 [PDF]

EFSA Journal, 2021
This assessment addresses a food enzyme preparation consisting of the immobilised intact but non‐viable cells of the genetically modified Corynebacterium glutamicum strain FIS002 by CJ‐Tereos Sweeteners Europe SAS. The production strain produces the food
EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP), Claude Lambré, José Manuel Barat Baviera, Claudia Bolognesi, Pier Sandro Cocconcelli, Riccardo Crebelli, David Michael Gott, Konrad Grob, Evgenia Lampi, Marcel Mengelers, Alicja Mortensen, Gilles Rivière, Inger‐Lise Steffensen, Christina Tlustos, Henk Van Loveren, Laurence Vernis, Holger Zorn, Boet Glandorf, Lieve Herman, Yi Liu, Joaquim Maia, Elsa Nielsen, Andrew Chesson   +22 more
doaj   +10 more sources

Safety evaluation of the food enzyme d‐psicose 3‐epimerase from the genetically modified Escherichia coli strain K‐12 W3110 (pWKLP) [PDF]

EFSA Journal, 2021
The food enzyme d‐psicose 3‐epimerase (EC 5.1.3.30) is produced with the genetically modified Escherichia coli strain K‐12 W3110 (pWKLP) by Matsutani Chemical Industry Co., Ltd.
EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP), Claude Lambré, José Manuel Barat Baviera, Claudia Bolognesi, Pier Sandro Cocconcelli, Riccardo Crebelli, David Michael Gott, Konrad Grob, Evgenia Lampi, Marcel Mengelers, Alicja Mortensen, Gilles Rivière, Inger‐Lise Steffensen, Christina Tlustos, Henk van Loveren, Laurence Vernis, Holger Zorn, Boet Glandorf, Lieve Herman, Magdalena Andryszkiewicz, Ana Gomes, Yi Liu, Joaquim Maia, Sandra Rainieri, Andrew Chesson   +24 more
doaj   +11 more sources

Production, purification, characterization, and safety evaluation of constructed recombinant D-psicose 3-epimerase [PDF]

Microbial Cell Factories
Background D-psicose 3-epimerase (DPEase) is a potential catalytic enzyme for D-psicose production. D-psicose, also known as D-allulose, is a low-calorie sweetener that has gained considerable attention as a healthy alternative sweetener due to its ...
Nisit Watthanasakphuban, Pimsiriya Srila, Phitsanu Pinmanee, Charatda Punvittayagul, Nopphon Petchyam, Boontiwa Ninchan   +5 more
doaj   +5 more sources

Improved Performance of D-Psicose 3-Epimerase by Immobilisation on Amino-Epoxide Support with Intense Multipoint Attachment [PDF]

Foods, 2021
D-allulose is an epimer of D-fructose at the C-3 position. With similar sweetness to sucrose and a low-calorie profile, D-allulose has been considered a promising functional sweetener.
Yifan Bu, Tao Zhang, Bo Jiang, Jingjing Chen   +3 more
doaj   +6 more sources

Highly efficient production of Clostridium cellulolyticum H10 d-psicose 3-epimerase in Bacillus subtilis and use of these cells to produce d-psicose [PDF]

Microbial Cell Factories, 2018
Background d-Psicose 3-epimerase (DPEase) catalyzes the isomerization of d-fructose to the rare sugar d-psicose, which may help prevent obesity, reduce blood sugar and blood fat, and inhibit intra-abdominal fat accumulation.
Lingqia Su, Fan Sun, Zhanzhi Liu, Kang Zhang, Jing Wu   +4 more
doaj   +6 more sources

Characterization of a novel metal-dependent D-psicose 3-epimerase from Clostridium scindens 35704. [PDF]

PLoS ONE, 2013
The noncharacterized protein CLOSCI_02528 from Clostridium scindens ATCC 35704 was characterized as D-psicose 3-epimerase. The enzyme showed maximum activity at pH 7.5 and 60°C.
Wenli Zhang, Dan Fang, Qingchao Xing, Leon Zhou, Bo Jiang, Wanmeng Mu   +5 more
doaj   +6 more sources

In Silico Analysis and Development of the Secretory Expression of D-Psicose-3-Epimerase in Escherichia coli [PDF]

Microorganisms
D-psicose-3-epimerase (DPEase), a key enzyme for D-psicose production, has been successfully expressed in Escherichia coli with high yield.
Nisit Watthanasakphuban, Boontiwa Ninchan, Phitsanu Pinmanee, Kittipong Rattanaporn, Suttipun Keawsompong   +4 more
doaj   +5 more sources

Streamlined production of immobilized D-psicose 3-epimerase via secretion in Pichia pastoris: a new paradigm for industrial D-psicose production [PDF]

Microbial Cell Factories
Background D-psicose, a rare sugar with significant health benefits, holds great promise as a low-calorie sweetener. Its synthesis requires the enzyme called D-psicose 3-epimerase (DPEase), which converts D-fructose into D-psicose.
Pimsiriya Srila, Phitsanu Pinmanee, Boontiwa Ninchan, Nisit Watthanasakphuban   +3 more
doaj   +4 more sources

Adaptive Steered Molecular Dynamics Combined With Protein Structure Networks Revealing the Mechanism of Y68I/G109P Mutations That Enhance the Catalytic Activity of D-psicose 3-Epimerase From Clostridium Bolteae [PDF]

Frontiers in Chemistry, 2018
The scarcity, richness, and other important physiological functions of D-psicose make it crucial to increase the yield of D-psicose. The production of D-psicose can be accomplished by D-psicose 3-epimerase (DPEase) from Clostridium bolteae (CbDPEase ...
Jingxuan Zhu, Yi Li, Jinzhi Wang, Zhengfei Yu, Ye Liu, Yi Tong, Weiwei Han   +6 more
doaj   +7 more sources

A food-grade expression system for d-psicose 3-epimerase production in Bacillus subtilis using an alanine racemase-encoding selection marker. [PDF]

Bioresour Bioprocess, 2017
BackgroundFood-grade expression systems require that the resultant strains should only contain materials from food-safe microorganisms, and no antibiotic resistance marker can be utilized.
Chen J, Jin Z, Gai Y, Sun J, Zhang D.
europepmc   +6 more sources