Results 111 to 120 of about 218 (163)
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Lack of specificity of DA/DAPI fluorescence
Clinical Genetics, 1990Cytogenetic studies showed 47,XY, + mar in a developmentally retarded child with some features of Prader‐Willi syndrome, and 46,XX in his mother. The marker chromosome showed a single subterminal primary constriction, bisatellites, and two C‐bands. DA/DAPI staining showed two intense bands in the marker chromosome, which most likely was derived from ...
Miriam G. Wilson+3 more
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Characterization of a DAPI-RIT-DAPI System for Gas-Phase Ion/Molecule and Ion/Ion Reactions
Journal of the American Society for Mass Spectrometry, 2013The discontinuous atmospheric pressure interface (DAPI) has been developed as a facile means for efficiently introducing ions generated at atmospheric pressure to an ion trap in vacuum [e.g., a rectilinear ion trap (RIT)] for mass analysis. Introduction of multiple beams of ions or neutral species through two DAPIs into a single RIT has been previously
Ziqing Lin+7 more
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The present work follows our preliminary discovery of biphenyl diarylpyrimidines (DAPYs) as HIV-1 nonnucleoside reverse transcriptase inhibitors. Further structural optimization of biphenyl-DAPYs led to the identification of a new series of biphenyl ...
Ya-Li Sang+6 more
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FISH Detection on DAPI-Banded Chromosomes
2003link_to_subscribed_fulltext
Henry H.Q. Heng+3 more
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An Iodinated DAPI‐Based Reagent for Mass Cytometry
ChemBioChem, 2020AbstractMultiparametric single‐cell analysis has seen dramatic improvements with the introduction of mass cytometry (MC) and imaging mass cytometry (IMC™). These technologies expanded the number of biomarkers that can be identified simultaneously by using heavy‐isotope‐tagged antibody reagents.
Rahul Rana+5 more
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Reverse fluorescent chromosome banding with chromomycin and DAPI [PDF]
Two DNA binding guanine-specific antibiotics, chromomycin A3 (CMA) and the closely related mithramycin (MM), were used as chromosome fluorescent dyes. Root-tip metaphase chromosomes of three plant species and human metaphase chromosomes were sequentially stained with CMA or MM and the DNA binding AT-specific fluorochrome 4'-6-diamidino-2-phenylindole ...
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The DAPI-3 amacrine cells of the rabbit retina
Visual Neuroscience, 1997AbstractIn the rabbit retina, the nuclear dye, 4,6, diarnidino-2-phenylindole (DAPI), selectively labels a third type of amacrine cell, in addition to the previously characterized type a and type b cholinergic amacrine cells. In this study, these “DAPI-3” amacrine cells have been characterized with respect to their somatic distribution, dendritic ...
Wright, Layne L.+5 more
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Role of DAPI in microtubule reactions at steady-state
Biochemical and Biophysical Research Communications, 1985The new fluorophor for tubulin, DAPI, is shown to bind to a site different from the exchangeable nucleotide binding site (E site) and to inhibit GTP hydrolysis by the tubulin-colchicine complex within an uncompetitive scheme. Moreover the dissociation rate constant of tubulin for microtubule ends at 32 degrees C was found largely decreased in the ...
Catherine Heusele, Dominique Bonne
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THE ENUMERATION OF AQUATIC BACTERIA USING DAPI [PDF]
SUMMARY A comparison was made between acridine orange (AO) and 4'6-diamidino-2-phenylindole (DAPI), a highly sensitive DNA fluorescing stain, for enumerating aquatic bacteria. When water samples were processed in a darkened room and a final DAPI concentration of 0,05 μg ml −1 was used, there was no significant difference between the population ...
Lyn M. Sephton, Richard D. Robarts
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DAPI: a DNA-Specific Fluorescent Probe
Biotechnic & Histochemistry, 1995DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids.
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