N4BP1 uses tandem KH domains to associate with EDC4 and mRNA decapping factors in P-bodies [PDF]
Communications BiologyProcessing bodies (P-bodies) are cytoplasmic, non-membrane-bound structures involved in mRNA decay. EDC4 serves as a key scaffold for the decapping complex within P-bodies. Here, we demonstrate that N4BP1 interacts with EDC4, as well as with DCP1A, DCP2,
Paweł Piłat +4 more
doaj +2 more sources
RNA anchoring of Upf1 facilitates recruitment of Dcp2 in the NMD decapping complex. [PDF]
Nucleic Acids ResInternational audienceUpf1 RNA helicase is a pivotal factor in the conserved nonsense-mediated mRNA decay (NMD) process. Upf1 is responsible for coordinating the recognition of premature termination codons (PTCs) in a translation-dependent manner and ...
Ruiz-Gutierrez N +7 more
europepmc +4 more sources
Human DCP1 is crucial for mRNA decapping and possesses paralog-specific gene regulating functions [PDF]
eLifeThe mRNA 5'-cap structure removal by the decapping enzyme DCP2 is a critical step in gene regulation. While DCP2 is the catalytic subunit in the decapping complex, its activity is strongly enhanced by multiple factors, particularly DCP1, which is the ...
Ting-Wen Chen +7 more
doaj +2 more sources
Degron models: a toolbox for rapid in vivo depletion of essential proteins regulating mRNA metabolism [PDF]
Communications BiologyDue to their essentiality, studying proteins involved in fundamental processes in vivo is challenging. PROTAC-based systems offer time-controlled protein depletion, but their characterization in vivo remains limited. Here, with an efficient direct zygote
Wiktor Antczak +7 more
doaj +2 more sources
Dcp2: an mRNA decapping enzyme that adopts many different shapes and forms
Current Opinion in Structural Biology, 2019 Eukaryotic mRNAs contain a 5' cap structure that protects the transcript against rapid exonucleolytic degradation. The regulation of cellular mRNA levels therefore depends on a precise control of the mRNA decapping pathways.
Sprangers, Remco, Wurm, Jan Philip
core +4 more sources
A protein-proximity screen reveals Ebola virus co-opts the mRNA decapping complex through the scaffold protein EDC4 [PDF]
Nature CommunicationsThe interaction of host and Ebola virus (EBOV) proteins is required for establishing infection. In this study, we use proximity-dependent biotinylation to identify cellular proteins that bind to EBOV proteins encoded by six of the seven viral genes. Hits
Callie J. Donahue +12 more
doaj +2 more sources
PLoS Genetics, 2018 Scd6 protein family members are evolutionarily conserved components of translationally silent mRNA granules. Yeast Scd6 interacts with Dcp2 and Dhh1, respectively a subunit and a regulator of the mRNA decapping enzyme, and also associates with ...
Quira Zeidan +5 more
doaj +2 more sources
Non-redundant roles for the human mRNA decapping cofactor paralogs DCP1a and DCP1b [PDF]
Life Science AllianceThe study represents the first functional dissection of the two human cofactors present in the RNA decapping complex, and, significantly, it evaluates their role in the transcript buffering system.
Ivana Vukovic +6 more
doaj +2 more sources
Morpho-phytochemical screening and biological assessments of aerial parts of Iranian populations of wild carrot (Daucus carota L. subsp. carota) [PDF]
Scientific ReportsThis study investigated the morphological and phytochemical characteristics of 118 genotypes of Daucus carota L. subsp. carota, collected from natural habitats in five populations across West Azerbaijan.
Leila Dehghani +2 more
doaj +2 more sources
Genome-wide mapping of DCP2-dependent 5' cap footprints in <i>Arabidopsis thaliana</i>. [PDF]
NAR Genom BioinformMessenger RNA (mRNA) decapping mediated by DCP2 is a key mechanism controlling RNA stability and gene expression in eukaryotes, including plants. Despite its central role in regulating plant development and stress responses, the repertoire of mRNA 5 ...
Shukla N +4 more
europepmc +2 more sources