Results 81 to 90 of about 60,554,138 (296)

Semantic Assembly and Annotation of Draft RNAseq Transcripts without a Reference Genome. [PDF]

PLoS ONE, 2015
Transcriptomes are one of the first sources of high-throughput genomic data that have benefitted from the introduction of Next-Gen Sequencing. As sequencing technology becomes more accessible, transcriptome sequencing is applicable to multiple organisms ...
Andrey Ptitsyn, Ramzi Temanni, Christelle Bouchard, Peter A V Anderson   +3 more
doaj   +1 more source

RNA Sequencing Resolves Cryptic Pathogenic Variants in Mitochondrial Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Mitochondrial diseases are the most common inherited metabolic disorders, characterized by pronounced clinical and genetic heterogeneity that complicates molecular diagnosis. Although DNA‐based sequencing approaches have become standard in genetic testing, up to half of patients remain without a definitive diagnosis.
Zhimei Liu, Xin Duan, Fatemeh Peymani, Jia Wang, Chengjia Bao, Chaolong Xu, Ying Zou, Zixuan Zhang, Yunxi Zhang, Tongyue Li, Martin Pavlov, Junling Wang, Minhan Song, Tianyu Song, Xiaodi Han, Mingxi Sun, Danmin Shen, Ruoyu Duan, Huafang Jiang, Manting Xu, Holger Prokisch, Fang Fang   +21 more
wiley   +1 more source

Chloroplast genome assembly approaches from NGS data

Journal of BioScience and Biotechnology, 2016
The advent of Next Generation Sequencing platforms led to increase of research in whole genome assembly algorithms and software. Illumina Genome Analyzer produces a large amount of sequencing data, with a shorted read length, higher coverage and ...
Zdravka Ivanova, Evelina Daskalova, Georgi Minkov, Vesselin Baev   +3 more
doaj  

Accurate long-read de novo assembly evaluation with Inspector

Genome Biology, 2021
Long-read de novo genome assembly continues to advance rapidly. However, there is a lack of effective tools to accurately evaluate the assembly results, especially for structural errors.
Yu Chen, Yixin Zhang, Amy Y. Wang, Min Gao, Zechen Chong   +4 more
doaj   +1 more source

Self‐Healing Hydrogel‐Enabled Modular Assembly of Bilayered Skin Construct for Hair Follicle Regeneration

Advanced Healthcare Materials, EarlyView.
Self‐healing assembly of two cell‐laden hydrogel layers creates a biomimetic skin construct that restores both dermal and epidermal functions, resulting in accelerated wound healing and enhanced hair follicle regeneration in a full‐thickness skin wound model. ABSTRACT Alopecia remains a pervasive clinical challenge, largely owing to the limited ability
JaeWook Park, Su Yong Kim, MinSu Jeong, Beom Joon Kim, Shin Hyuk Kang, Won‐Gun Koh   +5 more
wiley   +1 more source

RNAi in the Rhizarian Phytopathogen Plasmodiophora brassicae: The Causal Agent of Clubroot Disease in Cruciferous Crops

Advanced Science, EarlyView.
This study uncovers an unusual RNAi pathway in the rhizarian pathogen Plasmodiophora brassicae. In the absence of Dicer, a Drosha‐like RNase III protein supports the biogenesis of predominant 21‐nt small RNAs, and two Argonaute proteins mediate small RNA‐guided silencing.
Xiong Zhang, Yupo Wu, Jiachang Hou, Lixia Gao, Longqi Pan, Cuicui Shen, Chuanji Zhao, Feng Gao, Yi Zhang, Li Ren, Xiaoping Fang, Xiaoling Dun, Shengyi Liu, Lijiang Liu   +13 more
wiley   +1 more source

Autism and mild epilepsy associated with a de novo missense pathogenic variant in the GTPase effector domain of DNM1

American Journal of Medical Genetics Part C: Seminars in Medical Genetics, EarlyView., 2023
Abstract Dynamin 1 is a GTPase protein involved in synaptic vesicle fission, which facilitates the exocytosis of neurotransmitters necessary for normal signaling. Pathogenic variants in the DNM1 gene are associated with intractable epilepsy, often manifested as infantile spasms at onset, developmental delay, and a movement disorder, and are located in ...
Davide Mei, Elena Parrini, Claudia Bianchini, Maria Luisa Ricci, Renzo Guerrini   +4 more
wiley   +1 more source

npbhavya/microbial-genome-assembly:

, 2022
Scripts and code used for genome assembly of bacterial ...
Bhavya Papudeshi
core   +1 more source

FAssem: FPGA Based Acceleration of De Novo Genome Assembly [PDF]

2013 IEEE 21st Annual International Symposium on Field-Programmable Custom Computing Machines, 2013
Next generation sequencing technologies produce large amounts of data at very low cost. They produce short reads of DNA fragments. These fragments have many overlaps, lots of repeats and may also include sequencing errors. The assembly process involves merging these sequences to form the original sequences.
Varma, Sharat Chandra, Kolin, Paul, Balakrishnan, M., Lavenier, Dominique   +3 more
openaire   +3 more sources

Illumina TruSeq synthetic long-reads empower de novo assembly and resolve complex, highly-repetitive transposable elements. [PDF]

PLoS ONE, 2014
High-throughput DNA sequencing technologies have revolutionized genomic analysis, including the de novo assembly of whole genomes. Nevertheless, assembly of complex genomes remains challenging, in part due to the presence of dispersed repeats which ...
Rajiv C McCoy, Ryan W Taylor, Timothy A Blauwkamp, Joanna L Kelley, Michael Kertesz, Dmitry Pushkarev, Dmitri A Petrov, Anna-Sophie Fiston-Lavier   +7 more
doaj   +1 more source