Results 81 to 90 of about 111,403 (262)

Evaluating the fidelity of de novo short read metagenomic assembly using simulated data. [PDF]

PLoS ONE, 2011
A frequent step in metagenomic data analysis comprises the assembly of the sequenced reads. Many assembly tools have been published in the last years targeting data coming from next-generation sequencing (NGS) technologies but these assemblers have not ...
Miguel Pignatelli, Andrés Moya
doaj   +1 more source

Dynamic Regulation of Endogenous Transcription Factor Hubs at Single‐Molecule Resolution

Advanced Science, EarlyView.
This study combines single‐molecule microscopy and genome editing to characterize the dynamic behaviors of endogenous oncofusion transcription factor EWS::FLI1 in Ewing sarcoma cells. EWS::FLI1 forms neomorphic hubs that dynamically assemble and dissolve. The hubs are regulated during mitosis, by RNA, and by specific chemicals.
Shawn Yoshida, Yanghao Zhong, Akiko Kumagai, William G. Dunphy, Shasha Chong   +4 more
wiley   +1 more source

CAPRG: sequence assembling pipeline for next generation sequencing of non-model organisms. [PDF]

PLoS ONE, 2012
Our goal is to introduce and describe the utility of a new pipeline "Contigs Assembly Pipeline using Reference Genome" (CAPRG), which has been developed to assemble "long sequence reads" for non-model organisms by leveraging a reference genome of a ...
Arun Rawat, Mohamed O Elasri, Kurt A Gust, Glover George, Don Pham, Leona D Scanlan, Chris Vulpe, Edward J Perkins   +7 more
doaj   +1 more source

A Non‐Canonical Core Transcriptional Regulatory Circuit Orchestrates Chromatin Reprogramming to Drive Osimertinib Resistance in Non‐Small Cell Lung Cancer

Advanced Science, EarlyView.
A non‐canonical core transcriptional regulatory circuit, composed of ID3, SMAD3, and NR2F2, drives Osimertinib resistance in non‐small cell lung cancer through super‐enhancer‐mediated activation of EPAS1, which couples neuroendocrine differentiation with ferroptosis evasion.
Aochu Liu, Zhenguo Liu, Lizhen Jiang, Andong Huang, Xinqing Lin, Xinyue Li, Bingyuan Liu, Shiwen Hu, Qiang Pan, Junjun Huang, Liyuan Yin, Liling Jiang, Wei Yi, Wenjun Mao, Yueyuan Zheng, Xianping Shi   +15 more
wiley   +1 more source

The effect of variant interference on de novo assembly for viral deep sequencing

BMC Genomics, 2020
Background Viruses have high mutation rates and generally exist as a mixture of variants in biological samples. Next-generation sequencing (NGS) approaches have surpassed Sanger for generating long viral sequences, yet how variants affect NGS de novo ...
Christina J. Castro, Rachel L. Marine, Edward Ramos, Terry Fei Fan Ng   +3 more
doaj   +1 more source

NFYB Integrates Hormonal Signals into Tissue Allometry by Promoting Protein Biosynthesis

Advanced Science, EarlyView.
In the American cockroach, NFYB acts as a spatiotemporin that translates distinct hormonal cues into tissue‐specific allometry. Juvenile hormone activates NFYB in the early fat body, while 20‐hydroxyecdysone induces it in late wing pads. NFYB then promotes protein biosynthesis via core translational machinery, driving differential growth across the ...
Fangfang Liu, Shiming Zhu, Sishi Xia, Xiaoshuai Zhang, Qin Li, Zhanquan Zeng, Yuqing Liu, Wanyin Liang, Guanyu Zhou, Erxia Du, Suning Liu, Sheng Li   +11 more
wiley   +1 more source

DOGMA: de novo assembly of densely labelled optical DNA maps using a matrix profile approach.

PLoS ONE
In optical genome mapping (OGM), large numbers of individual DNA maps-sequence-specific data series along single DNA molecules-are produced. Such individual maps have to be stitched together in a process called de novo OGM assembly in order to create ...
Albertas Dvirnas, Luis Mario Leal-Garza, Zahra Abbaspour, Erik Fröbrant, Karolin Frykholm, Marie Wrande, Linus Sandegren, Fredrik Westerlund, Tobias Ambjörnsson   +8 more
doaj   +1 more source

PlantGFM: A Genomic Foundation Model for Discovery and Creation of Plant Genes

Advanced Science, EarlyView.
A plant genomic foundation model pre‐trained on 12 species enables both accurate gene prediction and de novo gene design. Through AI‐human knowledge screening, seven designed sequences showed transcriptional activity in plants, with two expressing stable proteins—demonstrating the first DNA‐RNA‐protein expression of LLM‐generated genes in plants and ...
Changhao Li, Qizhe Zhang, Hanchen Chen, Kepeng Lin, Chengfang Luo, Mengying Yang, Wei Xu, Fan Yao, Jianbing Yan, Qing‐Yong Yang, Xuehai Hu   +10 more
wiley   +1 more source

CEBPG‐Mediated Palmitic Acid Adaptation of Cancer‐Associated Fibroblasts Drives Metastasis of Oral Squamous Cell Carcinoma

Advanced Science, EarlyView.
This study reveals a metabolic signaling axis in the OSCC microenvironment where palmitic acid (PA) drives the activation of CAFs. PA uptake triggers CEBPG‐dependent epigenetic remodeling to upregulate ERN1 and TMBIM6, thereby mitigating ER stress. This adaptive program sustains CAF survival and the pro‐metastatic phenotype, establishing this pathway ...
Yiling Duan, Yitong Li, Yufei Wu, Xiao Yang, Rui Li, Hui Zhao, Zhengjun Shang   +6 more
wiley   +1 more source

PacBio But Not Illumina Technology Can Achieve Fast, Accurate and Complete Closure of the High GC, Complex Burkholderia pseudomallei Two-Chromosome Genome

Frontiers in Microbiology, 2017
Although PacBio third-generation sequencers have improved the read lengths of genome sequencing which facilitates the assembly of complete genomes, no study has reported success in using PacBio data alone to completely sequence a two-chromosome bacterial
Jade L. L. Teng, Jade L. L. Teng, Jade L. L. Teng, Jade L. L. Teng, Man Lung Yeung, Man Lung Yeung, Man Lung Yeung, Man Lung Yeung, Elaine Chan, Lilong Jia, Chi Ho Lin, Yi Huang, Herman Tse, Herman Tse, Herman Tse, Herman Tse, Samson S. Y. Wong, Samson S. Y. Wong, Samson S. Y. Wong, Samson S. Y. Wong, Pak Chung Sham, Pak Chung Sham, Susanna K. P. Lau, Susanna K. P. Lau, Susanna K. P. Lau, Susanna K. P. Lau, Susanna K. P. Lau, Patrick C. Y. Woo, Patrick C. Y. Woo, Patrick C. Y. Woo, Patrick C. Y. Woo, Patrick C. Y. Woo   +31 more
doaj   +1 more source