Results 31 to 40 of about 18,296 (185)

Determination of Deamidation in Adjuvanted Vaccine Antigens through Isoaspartic Acid Quantification

Vaccines
Deamidation is a post-translational chemical modification that occurs within proteins and can be influenced by many factors, including temperature and pH. In vaccines, deamidation is considered undesirable as it may lead to changes in structure, function,
Manvi Hasija, Jian Ma, Bing Li, Nausheen Rahman, Kirsten A. Strahlendorf, Salvador Fernando Ausar   +5 more
doaj   +1 more source

Applications of Enzyme Technology to Enhance Transition to Plant Proteins: A Review

Foods, 2023
As the plant-based food market grows, demand for plant protein is also increasing. Proteins are a major component in foods and are key to developing desired structures and textures.
Ourania Gouseti, Mads Emil Larsen, Ashwitha Amin, Serafim Bakalis, Iben Lykke Petersen, Rene Lametsch, Poul Erik Jensen   +6 more
doaj   +1 more source

Human Triosephosphate Isomerase Is a Potential Target in Cancer Due to Commonly Occurring Post-Translational Modifications

Molecules, 2023
Cancer involves a series of diseases where cellular growth is not controlled. Cancer is a leading cause of death worldwide, and the burden of cancer incidence and mortality is rapidly growing, mainly in developing countries. Many drugs are currently used,
Sergio Enríquez-Flores, Ignacio De la Mora-De la Mora, Itzhel García-Torres, Luis A. Flores-López, Yoalli Martínez-Pérez, Gabriel López-Velázquez   +5 more
doaj   +1 more source

Production of Highly Active Recombinant Dermonecrotic Toxin of Bordetella Pertussis

Toxins, 2020
Pathogenic Bordetella bacteria release a neurotropic dermonecrotic toxin (DNT) that is endocytosed into animal cells and permanently activates the Rho family GTPases by polyamination or deamidation of the glutamine residues in their switch II regions (e ...
Ondrej Stanek, Irena Linhartova, Jana Holubova, Ladislav Bumba, Zdenko Gardian, Anna Malandra, Barbora Bockova, Shihono Teruya, Yasuhiko Horiguchi, Radim Osicka, Peter Sebo   +10 more
doaj   +1 more source

Development of an icIEF assay for monitoring AAV capsid proteins and application to gene therapy products

Molecular Therapy: Methods & Clinical Development, 2023
Adeno-associated virus (AAV) gene therapy vectors, which contain a DNA transgene packaged into a protein capsid, have shown tremendous therapeutic potential in recent years.
Xiaoping Z. He, Thomas W. Powers, Sisi Huang, Zhenjiu Liu, Heliang Shi, John D. Orlet, Jim J. Mo, Saipraveen Srinivasan, Steven Jacobs, Kun Zhang, Herbert A. Runnels, Melissa M. Anderson, Thomas F. Lerch   +12 more
doaj   +1 more source

Comprehensive Evaluation of Different TiO₂-Based Phosphopeptide Enrichment and Fractionation Methods for Phosphoproteomics

Cells, 2022
Protein phosphorylation is an essential post-translational modification that regulates multiple cellular processes. Due to their low stoichiometry and ionization efficiency, it is critical to efficiently enrich phosphopeptides for phosphoproteomics ...
Jiaran Li, Jifeng Wang, Yumeng Yan, Na Li, Xiaoqing Qing, Ailikemu Tuerxun, Xiaojing Guo, Xiulan Chen, Fuquan Yang   +8 more
doaj   +1 more source

Analytical and pharmacological consequences of the in vivo deamidation of trastuzumab and pertuzumab [PDF]

, 2022
A liquid chromatography-tandem mass spectrometry method is presented for the quantitative determination of the in vivo deamidation of the biopharmaceutical proteins trastuzumab and pertuzumab at an asparagine in their complementarity determining regions (
Sonke, Gabe S, Bischoff, Rainer; id_orcid, van de Merbel, Nico C, van der Voort, Anna, Bults, Peter; id_orcid, Meijer, Coby, Peter Bults, Anna van der Voort, Gabe S. Sonke, Coby Meijer, Rainer Bischoff, Nico C. van de Merbel   +11 more
core   +1 more source

Palaeoproteomic Deconvolution of Physical and Genetic Collagen Mixtures

Advanced Science, EarlyView.
ClassiCOL_v2 uses a novel “peptide patching” framework that treats any collagen sample as a patchwork of peptide sequences derived from phylogenetically related species. This allows the algorithm to simultaneously resolve two previously intractable problems: multi‐species samples and/or database deficiencies.
Ian Engels, Tristan Dedrie, Synnøve K. Mo, Simon Van de Vyver, Thijs R. A. Vandenbroucke, Kévin Di Modíca, Jan Decher, Alice Toso, Dieter Deforce, Simon Daled, Alexandra Burnett, Grégory Abrams, Maarten Dhaenens   +12 more
wiley   +1 more source

NEDD8 Deamidation Inhibits Cullin RING Ligase Dynamics

Frontiers in Immunology, 2021
Cullin-RING ligases (CRLs) are a significant subset of Ubiquitin E3 ligases that regulate multiple cellular substrates involved in innate immunity, cytoskeleton modeling, and cell cycle.
Priyesh Mohanty, Kiran Sankar Chatterjee, Ranabir Das   +2 more
doaj   +1 more source

Nascent Extracellular Matrix Converts Biomaterial Cues into Cell Fate Decisions

Advanced Science, EarlyView.
This study uncovers a tri‐directional interplay among cells, hydrogels, and their nascent extracellular matrix. Hydrogel norbornene modification alters CD44‐mediated cell–hydrogel interactions, which shape nascent matrix deposition, composition, and spatial organization.
Joshua Y. Liu, Ziqian Yang, Arina E. Velieva, Eleanor M. Plaster, Mingyue Fan, Donia W. Ahmed, Avinava Roy, Pamela Duran, Pamela Panovich, Alexandra S. Piotrowski‐Daspit, Carlos A. Aguilar, Megan L. Killian, Claudia Loebel   +12 more
wiley   +1 more source