Results 261 to 270 of about 63,047 (307)
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Thrombosis and Haemostasis, 1993
SummaryA new, simplified method of degranulating human platelets using the thrombin receptor peptide SFLLRN (20 |iM) is described; released fibrinogen cannot be converted to fibrin, and the platelets are not exposed to a proteolytic enzyme, as they are when thrombin is used for degranulation.
Margaret L. Rand +4 more
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SummaryA new, simplified method of degranulating human platelets using the thrombin receptor peptide SFLLRN (20 |iM) is described; released fibrinogen cannot be converted to fibrin, and the platelets are not exposed to a proteolytic enzyme, as they are when thrombin is used for degranulation.
Margaret L. Rand +4 more
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Measuring Degranulation of Mast Cells
Current Protocols in Immunology, 1993AbstractThis unit describes methods for measuring exocytosis of preformed mediators from secretory granules as an indication of IgE receptor‐mediated activation of mast cells. The first basic protocol describes the measurement of biogenic amines (serotonin and histamine) secreted by activated rodent mast cells (for serotonin) or rodent and human mast ...
Stephen C. Dreskin, Robert J. Hohman
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2017
Basophil degranulation assay has gained importance over the last decade in both diagnosis of food allergy and evaluation of progression of immunotherapy. This assay involves the identification and quantification of the expression of CD63 molecule on basophil membrane. CD63 is a marker of multivesicular bodies that is exposed to cell membrane during the
Mohanapriya Kamalakannan +2 more
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Basophil degranulation assay has gained importance over the last decade in both diagnosis of food allergy and evaluation of progression of immunotherapy. This assay involves the identification and quantification of the expression of CD63 molecule on basophil membrane. CD63 is a marker of multivesicular bodies that is exposed to cell membrane during the
Mohanapriya Kamalakannan +2 more
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Myeloperoxidase Stimulates Neutrophil Degranulation
Bulletin of Experimental Biology and Medicine, 2016Myeloperoxidase, heme enzyme of azurophilic granules in neutrophils, is released into the extracellular space in the inflammation foci. In neutrophils, it stimulates a dose-dependent release of lactoferrin (a protein of specific granules), lysozyme (a protein of specific and azurophilic granules), and elastase (a protein of azurophilic granules).
Alexey V. Sokolov +6 more
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Nephron, 2000
<i>Background/Aims: </i>Degranulation of polymorphonuclear leukocytes (PMN) during hemodialysis (HD) is usually assessed by measuring degranulation products. However, this process might also be estimated by the assessment of cell surface markers.
Grooteman, M P +6 more
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<i>Background/Aims: </i>Degranulation of polymorphonuclear leukocytes (PMN) during hemodialysis (HD) is usually assessed by measuring degranulation products. However, this process might also be estimated by the assessment of cell surface markers.
Grooteman, M P +6 more
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Neutrophil degranulation: therapeutic targets in [NTP]O mediated neutrophil degranulation
Medical Hypotheses, 2004Extracellular nucleotide-induced stimulation and activation of peripheral blood neutrophil and subsequent degranulation plays a critical role in immediate type hypersensitivity reaction and other inflammatory diseases. The extracellular nucleotides stimulate a P2Y receptor(s) on human PMN with the pharmacological profile similar to that of the P2Y2 ...
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Neutrophil Degranulation Induced by Haemophilus ducreyi
bchm, 2001Haemophilus ducreyi is the causative bacterium of genital ulcers, which are collectively known as chancroid. Little is known about the cytotoxicity of H. ducreyi. The virulent strains are relatively resistant to phagocytosis and apoptosis by neutrophils.
Anwar Hoosen +2 more
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Cannabinoid induced degranulation of rabbit neutrophils
Biochemical and Biophysical Research Communications, 1982Abstract We have examined the effects of various cannabinoids on the degranulation of rabbit peritoneal neutrophils. Several cannabinoids were found to cause a dose-dependent and noncytotoxic release of lysosomal enzymes from the neutrophils. The degranulation induced by cannabidiol is rapid ( t 1 2 = 2.3 min ), and enhanced by ...
Ramadan I. Sha'afi +9 more
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Optical Visualization of Microsomal Degranulation by a Carcinogen
Journal of Environmental Pathology, Toxicology and Oncology, 2001We report here for the first time the optical visualization of microsomal degranulation induced by a carcinogen (p-dimethylaminoazobenzene), employing analytical ultracentrifugation. Our observations demonstrate that electrophiles of a carcinogen can disrupt ribosome-membrane interaction in rough microsomes by their attacks on nucleophilic components ...
Harinder M. Dani, Parminder Kaur
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[44] Human neutrophil degranulation
1988Publisher Summary This chapter describes the techniques for isolating these subcellular organelles from normal, unstimulated human blood neutrophils and for measuring granule exocytosis by activated neutrophils. Neutrophils are wandering, phagocytic white blood cells that are critical to host defenses against bacterial and fungal infections. They are
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