Results 71 to 80 of about 65,216 (256)

Conformational transition of alkali-denatured soybean 7S and 11S globulins by ethanol. [PDF]

Agricultural and Biological Chemistry, 1980
Transformation depending on ethanol concentration of alkali-denatured soybean 7S and 11S globulins was studied by means of CD measurement and difference spectra. The ordered structure formation from unfolded random structure was enhanced with increasing ethanol concentration.
ISHINO, Keizo, KUDO, Shiro
openaire   +2 more sources

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Model‐Guided Design of Paper Membrane Architectures for Controlled Flow and Enhanced Diagnostic Sensitivity

Advanced Engineering Materials, EarlyView.
Geometrical modifications on nitrocellulose membranes can result in observable differences in signal intensity relative to a reference membrane (top and bottom left). Using two reference experiments, we have developed a theoretical model that can reproduce the experimentally observed flow changes and provide information on membrane parameters, e.g ...
Maria Dimaki, Lea Holritz, Winnie E. Svendsen   +2 more
wiley   +1 more source

Thermal stability of pepsin: A predictive thermodynamic model of a multi-domain protein

Biochemistry and Biophysics Reports, 2017
Pepsin is generally used in the preparation of F(ab)2 fragments from antibodies. The antibodies that are one of the largest and fastest growing categories of bio- pharmaceutical candidates.
Ali Asghar Rastegari, Behnaz Buzari, Abdol-Khalegh Bordbar   +2 more
doaj   +1 more source

Chemical analysis and protective effect of Elaeis guineensis kernel oil against calcium ion-induced mitochondrial membrane permeability transition

Journal of Basic and Applied Research in Biomedicine, 2022
This study evaluated the chemical property and effect of Elaeis guineensis kernel (EGK) oil on calcium ion-induced mitochondrial membrane permeability transition (MMPT) using standard in vitro methods. Results showed the EGK oil values for iodine (46.53±
God’swill Anyasor, Ezinwanne Onuoha, Gisarin Olusola   +2 more
doaj   +2 more sources

Engineering Nanoplatforms for Alzheimer's Disease Detection via Biomolecular Corona Proteomic and Lipidomic Profiling

Advanced Healthcare Materials, EarlyView.
Engineered nanoparticles capture disease‐specific biomolecular coronas that uncover hidden molecular features of Alzheimer's disease. Combined proteomic and lipidomic analyses reveal a characteristic shift in ribosomal machinery and energy metabolism, generating a multiomic fingerprint that supports accurate disease detection and opens new ...
Antonietta Greco, Roberto Baretta, Andrea Di Fonzo, Andrea Armirotti, Stefano Cinti, Mara Mirasoli, Marco Frasconi, Claudia Corbo   +7 more
wiley   +1 more source

Lonicera japonica‐Derived Nanovesicles Ameliorate Acute Lung Injury by Suppressing Neutrophil Extracellular Traps Formation in Mice

Advanced Healthcare Materials, EarlyView.
(A) Isolation and purification of LDNVs. (B) Establishment of the ALI model and LDNVs delivery. (C) Therapeutic mechanisms of LDNVs in treating ALI. This scheme figure was drawn by Figdraw, ID: IUIIO21222. ABSTRACT Acute lung injury (ALI) is a lethal respiratory disease.
Xi Lin, Jian Lin, Yezi Zhang, Xinyan Zhang, Xinghua Liu, Hongying Cao, Huan Shen, Jianming Liang, Zhenhua Dai, Siyuan Xu, Xingdong Lin   +10 more
wiley   +1 more source

Multi-Step Unfolding and Rearrangement of α-Lactalbumin by SDS Revealed by Stopped-Flow SAXS

Frontiers in Molecular Biosciences, 2020
Interactions between proteins and surfactants are both of fundamental interest and relevant for applications in food, cosmetics and detergency. The anionic surfactant sodium dodecyl sulfate (SDS) denatures essentially all proteins. Denaturation typically
Grethe Vestergaard Jensen, Grethe Vestergaard Jensen, Jannik Nedergaard Pedersen, Daniel E. Otzen, Daniel E. Otzen, Jan Skov Pedersen, Jan Skov Pedersen   +6 more
doaj   +1 more source

Eutectozymes as Soft Hybrid Materials for Advanced Biocatalysis

Advanced Materials, EarlyView.
Eutectozymes are sustainable hybrid materials that embed a GOx–HRP cascade within hydrophobic eutectogels featuring a dual supramolecular–covalent network. This architecture preserves native enzyme structure and stability, enables efficient heterogeneous biocatalysis in aqueous media, and positions eutectogels as robust platforms for next‐generation ...
Manuel Eduardo Martinez Cartagena, Lucia Suarez, Aitor Ontoria, Francisca J. Benitez, Elixabete Rezabal, María Soledad Orellano, Marcelo Calderón, Cristián Huck‐Iriart, Agustin S. Picco, Matias L. Picchio, Ana Beloqui   +10 more
wiley   +1 more source

The effects of temperature, feed ratio, and reaction time on the properties of copolymer PLA-PEG-PLA

Vietnam Journal of Science, Technology and Engineering, 2019
Triblock copolymers were polymerised by the ringopening reaction of D,L-lactide in the presence of poly(ethylene glycol) (PEG), with number-average molecular weight (Mn) of 1500 and 2050 g/mol, using Sn(Oct)2 as a catalyst.
Viet Linh Nguyen-Vu, Mai Anh Pham, Dai Phu Huynh   +2 more
doaj   +1 more source