Results 41 to 50 of about 101,166 (300)

Deoxyribonuclease Treatment Improves the Homogeneity of Single-Stranded DNA Preparations

open access: yesBioTechniques, 1997
The isolation of single-stranded (ss) phagemid DNA using standard protocols often results in impure preparations, which contain undesirable quantities of chromosomal and/or double-stranded (ds) phagemid DNA.
T.K. Aliev   +3 more
doaj   +1 more source

Fragmentomics of urinary cell-free DNA in nuclease knockout mouse models.

open access: yesPLoS Genetics, 2022
Urinary cell-free DNA (ucfDNA) is a potential biomarker for bladder cancer detection. However, the biological characteristics of ucfDNA are not well understood.
Meihui Chen   +18 more
doaj   +1 more source

DNA in fresh urine supernatant is not affected by additional centrifugation and is protected against deoxyribonuclease

open access: yesMolecular and Cellular Probes, 2023
Urinary DNA is widely studied as a non-invasive marker for monitoring of kidneys after transplantation or the progression of urinary tract tumors. The quantity of urinary DNA especially of mitochondrial origin has been reported to mirror kidney damage in
Ľubica Janovičová   +4 more
doaj   +1 more source

Deoxyribonuclease 1 reduces pathogenic effects of cigarette smoke exposure in the lung

open access: yesScientific Reports, 2017
Our aim was to investigate if deoxyribonuclease (DNase) 1 is a potential therapeutic agent to reduce pathogenic effects of cigarette smoke exposure in the lung. Cigarette smoke causes protease imbalance with excess production of proteases, which is a key
P. King   +15 more
semanticscholar   +1 more source

Intrapleural therapy for pleural infection from bronchopleural fistula in an adult with hyper‐IgE syndrome

open access: yesRespirology Case Reports, 2023
We presented the case of an adult patient with hyper‐IgE syndrome (HIES) who was admitted acutely with a large hydropneumothorax from lung consolidation, a bronchopleural fistula and pleural infection.
Sam Faber   +3 more
doaj   +1 more source

The inhibition of deoxyribonuclease I by hydroxybiphenyls [PDF]

open access: greenBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
Abstract DNA extracted with certain commercial brands of phenol is resistant to hydrolysis by the endonuclease deoxyribonuclease I, while DNA extracted with other brands, or prepared by NaCl extraction, is susceptible to hydrolysis. The agent responsible for inhibition has been shown to be an oxidation product produced in some phenols.
Joel Gottesfeld   +4 more
openalex   +5 more sources

Purification and characterization of deoxyribonuclease from small intestine of camel Camelus dromedarius

open access: yesJournal of Genetic Engineering and Biotechnology, 2017
The chromatography of deoxyribonuclease (DNase) from small intestine of camel Camelus dromedarius by DEAE-Sepharose separated three isoforms DNase 1, DNase 2 and DNase 3. The DNase 3 was purified to homogeneity by chromatography on Sephacryl S-200.
Somia S. Abdel-Gany   +3 more
doaj   +1 more source

Effects of nucleases on cell-free extrachromosomal circular DNA

open access: yesJCI Insight, 2022
Cell-free extrachromosomal circular DNA (eccDNA) as a distinct topological form from linear DNA has recently gained increasing research interest, with possible clinical applications as a class of biomarkers.
Sarah T.K. Sin   +15 more
doaj   +1 more source

Studies on the Neutral Deoxyribonuclease Activity in Highly Purified Acid Deoxyribonuclease

open access: yesJournal of Biological Chemistry, 1972
Abstract The presence of neutral deoxyribonuclease activity in highly purified preparation of acid deoxyribonuclease from sheep spleen (deoxyribonuclease II, EC 3.1.4.6) is described. The activity at pH 7.0 differs from the activity at pH 4.5 in its optimal ionic strength, its inhibition by tRNA, inhibition by EDTA, and activation by divalent cations ...
Hanoch Slor, Tama Lev
openaire   +3 more sources

Redox metabolism correlates with cellular turnover and clinical phenotype of papillary thyroid carcinoma and colloid goiter

open access: yesArchives of Medical Science, 2019
Introduction Papillary thyroid carcinoma (PTC) and colloid goiter (CG) represent the most common thyroid malignant and benign diseases, respectively.
Branislav Rovcanin   +8 more
doaj   +1 more source

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