Results 61 to 70 of about 14,183,395 (235)

Impact of PEGylation on the mucolytic activity of recombinant human deoxyribonuclease I in cystic fibrosis sputum.

Clinical science, 2018
Highly viscous mucus and its impaired clearance characterize the lungs of patients with cystic fibrosis (CF). Pulmonary secretions of patients with CF display increased concentrations of high molecular weight components such as DNA and actin. Recombinant
Marie-Julie Guichard, D. Kinoo, A. Aubriot, N. Bauwens, Jordane Gougué, F. Vermeulen, P. Lebecque, T. Leal, R. Vanbever   +8 more
semanticscholar   +1 more source

Proteomic Investigations of Two Pakistani Naja Snake Venoms Species Unravel the Venom Complexity, Posttranslational Modifications, and Presence of Extracellular Vesicles

Toxins, 2020
Latest advancement of omics technologies allows in-depth characterization of venom compositions. In the present work we present a proteomic study of two snake venoms of the genus Naja i.e., Naja naja (black cobra) and Naja oxiana (brown cobra) of ...
Aisha Manuwar, Benjamin Dreyer, Andreas Böhmert, Anwar Ullah, Zia Mughal, Ahmed Akrem, Syed Abid Ali, Hartmut Schlüter, Christian Betzel   +8 more
doaj   +1 more source

Degradation of RNA during lysis of Escherichia coli cells in agarose plugs breaks the chromosome. [PDF]

PLoS ONE, 2017
The nucleoid of Escherichia coli comprises DNA, nucleoid associated proteins (NAPs) and RNA, whose role is unclear. We found that lysing bacterial cells embedded in agarose plugs in the presence of RNases caused massive fragmentation of the chromosomal ...
Sharik R Khan, Andrei Kuzminov
doaj   +1 more source

Src Reduces Neutrophil Extracellular Traps Generation and Resolves Acute Organ Damage

Advanced Science, EarlyView.
Src reduces neutrophil extracellular traps (NETs) generation and resolves acute organ damage. Src directly activates RAF1 by regulating phosphorylation at the Ser 621 site and mediates the RAF/MEK/ERK pathway, thereby affecting the intracellular ROS production. Alternatively, Src activates the RAF1/MEK/ERK pathway by mediating PKC phosphorylation.
Guotao Lu, Fei Han, Yaodong Wang, Chenchen Yuan, Qingtian Zhu, Tianqi Xia, Lin Chen, Xiaowu Dong, Yanbing Ding, Weiming Xiao, Yuyan Zhang, Jiajia Pan, Hongwei Xu, Weiwei Chen, Bo Tu, Wei Li, Fei Wang, Weijuan Gong, Lianghao Hu   +18 more
wiley   +1 more source

Enzymatic degradation of extracellular DNA exposed to chlorpyrifos and chlorpyrifos-methyl in an aqueous system

Environment International, 2019
The persistence of extracellular DNA (eDNA) is crucial for ensuring species diversity and ecological function in aquatic systems. However, scarce information exists about the impact of pesticides on eDNA, although they often co-exist in the aquatic ...
Bing Yang, Chao Qin, Xiaojie Hu, Kang Xia, Chao Lu, Fredrick Owino Gudda, Zhao Ma, Yanzheng Gao   +7 more
doaj   +1 more source

Protocol for TRAUMADORNASE: a prospective, randomized, multicentre, double-blinded, placebo-controlled clinical trial of aerosolized dornase alfa to reduce the incidence of moderate-to-severe hypoxaemia in ventilated trauma patients

Trials, 2020
Background Acute respiratory distress syndrome continues to drive significant morbidity and mortality after severe trauma. The incidence of trauma-induced, moderate-to-severe hypoxaemia, according to the Berlin definition, could be as high as 45%.
Julien Pottecher, Eric Noll, Marie Borel, Gérard Audibert, Sébastien Gette, Christian Meyer, Elisabeth Gaertner, Vincent Legros, Raphaël Carapito, Béatrice Uring-Lambert, Erik Sauleau, Walter G. Land, Seiamak Bahram, Alain Meyer, Bernard Geny, Pierre Diemunsch   +15 more
doaj   +1 more source

Circulating Level of Neutrophil Extracellular Traps Is Not a Useful Biomarker for Assessing Disease Activity in Antineutrophil Cytoplasmic Antibody-Associated Vasculitis. [PDF]

PLoS ONE, 2016
Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is a group of life-threatening disorders, and frequently affects the kidneys.
Huan Wang, Li-Li Sha, Tian-Tian Ma, Lu-Xia Zhang, Min Chen, Ming-Hui Zhao   +5 more
doaj   +1 more source

Single‐Cell Dissection of the Biological Function and Molecular Features Underlying the Micropeptide LSMEM1 in Kidney

Advanced Science, EarlyView.
LSMEM1, an evolutionarily conserved micropeptide with extreme hydrophobicity (aliphatic index═113) and dynamic amphiphilicity (GRAVY═0.017), features a strong α‐helical transmembrane anchor (residues 64‐86). Single‐cell analysis reveals its critical role in renal lipid homeostasis.
Peimin Liu, Hong Zhang, Shanzhi Yang, Jiaoqing Li, Danfeng Wu, Haosen Xu, Huan Jiang, Yi Jing, Guoxiang Jin, Ruobing Liu, Ning Fan, Xiaoyan Bai   +11 more
wiley   +1 more source

Ultrafast enzymatic digestion of deoxyribonucleic acid in aqueous microdroplets for sequence discrimination and identification

QRB Discovery, 2021
We report the use of aqueous microdroplets to accelerate deoxyribonucleic acid (DNA) fragmentation by deoxyribonuclease I (DNase I), and we present a simple, ultrafast approach named DNA fragment mass fingerprinting to discriminate different DNA ...
Xiaoqin Zhong, Hao Chen, Richard N. Zare
doaj   +1 more source

Inhibition of Macrophage ARID3A Alleviates Myocardial Ischemia‐Reperfusion Injury After Heart Transplantation by Reducing THBS1/CD47 Signaling‐Mediated Neutrophil Extracellular Traps Formation

Advanced Science, EarlyView.
This study reveals that ARID3A in macrophages can transcriptionally regulate the THBS1‐CD47 axis after heart transplantation (HT), thereby inducing activated neutrophils to release NETs through the p38 MAPK signaling pathway. The itaconate derivative 4‐OI can inhibit ARID3A in macrophages and promote the accumulation of antioxidant factors, thereby ...
Hao Tian, Yonghong Xiong, Junbiao Zhan, Zhikun Lu, Yuxi Zhang, Yan Leng, Qin Huang, Zhongyuan Xia   +7 more
wiley   +1 more source