Results 61 to 70 of about 170,315 (251)

Interaction of iodoacetamidofluorescein‐labelled tropomyosin with deoxyribonuclease I [PDF]

open access: yesFEBS Letters, 1989
5‐Iodoacetamidofluorescein (IAF) reacted with rabbit cardiac muscle tropomyosin (TM) to yield a highly fluorescent product, IAF‐TM. The extent of labelling reached one fluorescein group per TM molecule in solutions at pH 8.5. While fluorescence polarization values for IAF‐TM solutions were unaffected by the presence or absence of KCl, addition of ...
Burtnick, L.D., Wong, M.K.C.
openaire   +2 more sources

Back Again to the Future: A New Era for Cerebroprotection

open access: yesAnnals of Neurology, EarlyView.
Cerebroprotection is a fresh framework for designing neurological therapy that targets glia and vascular cells, in addition to neurons. In the future, successful cerebroprotection will involve targeting all elements of the neurovascular unit. Preclinical trials must include functional outcomes, as well as lesion morphometry.
Patrick Lyden
wiley   +1 more source

Enzymatic degradation of extracellular DNA exposed to chlorpyrifos and chlorpyrifos-methyl in an aqueous system

open access: yesEnvironment International, 2019
The persistence of extracellular DNA (eDNA) is crucial for ensuring species diversity and ecological function in aquatic systems. However, scarce information exists about the impact of pesticides on eDNA, although they often co-exist in the aquatic ...
Bing Yang   +7 more
doaj   +1 more source

The involvement of NETs in ANCA-associated vasculitis

open access: yesFrontiers in Immunology, 2023
Anti-neutrophil cytoplasmic autoantibody (ANCA)-associated vasculitis (AAV) is a serious autoimmune disease that is characterized by vascular necrosis.
Satoka Shiratori-Aso, Daigo Nakazawa
doaj   +1 more source

A Defined Agar Medium for Genetic Transformation of \u3cem\u3eNeisseria meningitidis\u3c/em\u3e [PDF]

open access: yes, 1962
Catlin, B. Wesley (Marquette University, Milwaukee, Wis.) and Gertrude M. Schloer. A defined agar medium for genetic transformation of Neisseria meningitidis. J. Bacteriol. 83:470–474.
Catlin, B. Wesley, Schloer, Gertrude M.
core   +1 more source

Preparation of nuclear matrices from cultured cells: subfractionation of nuclei in situ [PDF]

open access: yes, 1984
Analyses of the different structural systems of the nucleus and the proteins associated with them pose many problems. Because these systems are largely overlapping, in situ localization studies that preserve the in vivo location of proteins and cellular ...
Deppert, Wolfgang, Staufenbiel, Matthias
core   +2 more sources

Immune cells composition in the skin and subcutaneous adipose tissue of patients with systemic sclerosis

open access: yesJDDG: Journal der Deutschen Dermatologischen Gesellschaft, EarlyView.
Summary Background and objectives Systemic sclerosis (SSc) is a rare, chronic autoimmune disease characterized by fibrosis of the skin and/or internal organs. Emerging evidence suggests that subcutaneous adipose tissue may contribute to systemic inflammation and fibrosis in SSc.
Marija Geroldinger‐Simić   +3 more
wiley   +1 more source

Taxonomic and functional shifts in the microbiome of severely obese, prediabetic patients: Ketogenic diet versus energy‐matched standard diet

open access: yesDiabetes, Obesity and Metabolism, EarlyView.
Abstract Aims Obesity and type 2 diabetes mellitus (T2DM) are among the leading global health challenges of the 21st century. While caloric restriction remains the cornerstone of weight loss interventions, ketogenic diets (KD), characterised by low carbohydrate and high fat intake, have been shown to improve metabolic health partly by modulating the ...
June Stone   +5 more
wiley   +1 more source

Protocol for TRAUMADORNASE: a prospective, randomized, multicentre, double-blinded, placebo-controlled clinical trial of aerosolized dornase alfa to reduce the incidence of moderate-to-severe hypoxaemia in ventilated trauma patients

open access: yesTrials, 2020
Background Acute respiratory distress syndrome continues to drive significant morbidity and mortality after severe trauma. The incidence of trauma-induced, moderate-to-severe hypoxaemia, according to the Berlin definition, could be as high as 45%.
Julien Pottecher   +15 more
doaj   +1 more source

Degradation of RNA during lysis of Escherichia coli cells in agarose plugs breaks the chromosome. [PDF]

open access: yesPLoS ONE, 2017
The nucleoid of Escherichia coli comprises DNA, nucleoid associated proteins (NAPs) and RNA, whose role is unclear. We found that lysing bacterial cells embedded in agarose plugs in the presence of RNases caused massive fragmentation of the chromosomal ...
Sharik R Khan, Andrei Kuzminov
doaj   +1 more source

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