Results 141 to 150 of about 2,443,257 (268)

Development and validation of green spectrophotometric methods for simultaneous determination of etoricoxib and tramadol. [PDF]

Sci Rep
Al-Mutairi AA, Felemban RA, Alqahtani AS, Alnemari RM, Abduljabbar MH, Aldawsari MF, Ramzy S, Almalki AH.   +7 more
europepmc   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Enhanced Inhibition of <i>Trametes versicolor</i> by Structurally Modified Medicarpin: In Vitro Evaluation and In Silico Insights into Laccase Binding. [PDF]

Int J Mol Sci
Guevara-Martínez SJ, Rivera-Ramírez JD, Escutia-Gutiérrez R, Pérez-Cisneros MA, Villanueva-Mejía F, García-Zavala S, Herrera-Bucio R, Morales-Palacios FG.   +7 more
europepmc   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Artificial intelligence versus traditional approaches in multicomponent spectral analysis. [PDF]

Sci Rep
Fahmy NM, Obaydo RH, Lotfy HM.
europepmc   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Eco-friendly spectrophotometric approach for the determination of anti-diabetic drugs in fixed-dose formulation together with metformin's toxic impurity: comprehensive method assessment. [PDF]

Sci Rep
Mohamed DG, Abdelrahman MM, Ahmed AB, Magdy MA.   +3 more
europepmc   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Analytical investigation of soliton propagation in conformable fractional-order transmission line metamaterials. [PDF]

Sci Rep
Almetwally EM, Zayed MA, Abo-Hashem SI, Sadat R, Mabrouk SM, Rashed AS.   +5 more
europepmc   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source