Results 91 to 100 of about 3,538 (271)

Improving the Developability of an Antigen Binding Fragment by Aspartate Substitutions

, 2019
Aggregation can be a major challenge in the development of antibody-based pharmaceuticals as it can compromise the quality of the product during bioprocessing, formulation, and drug administration. To avoid aggregation, developability assessment is often
Per J. Greisen (6803348), Hanne S. Karkov (6803360), Kasper Huus (183813), Zoltan Bozoky (6803351), Laila I. Sakhnini, Maria B. Dainiak, Anja K. Pedersen (6803369), Per J. Greisen, Hanne S. Karkov, Laila I. Sakhnini (6803345), Charlotte Wiberg (1583677), Søren Lund, Jens-Jacob Hansen, Charlotte Wiberg, Søren Lund (6803354), Jens-Jacob Hansen (6803363), Zoltan Bozoky, Adriana-Michelle Wolf Perez, Nikolai Lorenzen, Leif Bülow, Leif Bülow (1618531), Nikolai Lorenzen (1835353), Kasper Huus, Maria B. Dainiak (6803366), Adriana-Michelle Wolf Perez (6803357), Anja K. Pedersen   +25 more
core   +2 more sources

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

IPRs, Technological Development, and Economic Development [PDF]

Journal of Economic Issues, 2006
textabstractIn the year 2000 some $142 billion in royalties were paid internationally by users of a specific piece of knowledge that were protected under Intellectual Property Right law (IPR) to those parties that owned these rights. Under current circumstances where knowledge & innovation play an increasingly significant role in the economy (Foray ...
openaire   +3 more sources

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Biologics developability data analysis using hierarchical clustering accelerates candidate lead selection, optimization, and preformulation screening

mAbs
Identification of an optimal single protein sequence at the discovery stage for preclinical and clinical development is critical to the rapid development and overall success of a biologic drug.
Kevin James Metcalf, Galen Wo, Jan Paulo Zaragoza, Fahimeh Raoufi, Jeanne Baker, Daoyang Chen, Mehabaw Derebe, Jason Hogan, Amy Hsu, Esther Kofman, David Leigh, Mandy Li, Dan Malashock, Cate Mann, Soha Motlagh, Jihea Park, Karthik Sathiyamoorthy, Madhura Shidhore, Yinyan Tang, Kevin Teng, Katharine Williams, Andrew Waight, Sultan Yilmaz, Fan Zhang, Huimin Zhong, Laurence Fayadat-Dilman, Marc Bailly   +26 more
doaj   +1 more source

In vitro and in silico assessment of the developability of a designed monoclonal antibody library. [PDF]

Despite major advances in antibody discovery technologies, the successful development of monoclonal antibodies (mAbs) into effective therapeutic and diagnostic agents can often be impeded by developability liabilities, such as poor expression, low ...

core   +2 more sources

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Toward enhancement of antibody thermostability and affinity by computational design in the absence of antigen

mAbs
Over the past two decades, therapeutic antibodies have emerged as a rapidly expanding domain within the field of biologics. In silico tools that can streamline the process of antibody discovery and optimization are critical to support a pipeline that is ...
Mark Hutchinson, Jeffrey A. Ruffolo, Nantaporn Haskins, Michael Iannotti, Giuliana Vozza, Tony Pham, Nurjahan Mehzabeen, Harini Shandilya, Keith Rickert, Rebecca Croasdale-Wood, Melissa Damschroder, Ying Fu, Andrew Dippel, Jeffrey J. Gray, Gilad Kaplan   +14 more
doaj   +1 more source