Results 111 to 120 of about 728 (155)
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Mechanism of dextran activation of dextransucrase
Carbohydrate Research, 1995Doman Kim, John F Robyt
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Modelling of oligosaccharide synthesis by dextransucrase
Biotechnology and Bioengineering, 1999Dextransucrase catalyses the formation of dextran, but also of numerous oligosaccharides from sucrose and different acceptors, if appropriate conditions are chosen. Much experimental work has been carried out and a scheme of reactions and a mathematical model have been developed to describe the complex kinetic behaviour of the enzyme.
B, Demuth, H J, Jördening, K, Buchholz
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The dissociation of aggregate forms of dextransucrase
Archives of Biochemistry and Biophysics, 1982Abstract Aggregate forms of dextransucrase obtained from Streptococcus sanguis dissociate in the presence of sodium dodecyl sulfate. However, the enzyme was unstable under these conditions. Nonionic detergents such as Triton X-100 stabilize the enzyme (A. W. Miller and J. R. Robyt, (1981) Fed. Proc. Fed. Amer. Soc. Exp. Biol.
G A, Luzio, D A, Grahame, R M, Mayer
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Activation and inhibition of dextransucrase by calcium
Biochimica et Biophysica Acta (BBA) - General Subjects, 1986Initial rate kinetics of dextran synthesis by dextransucrase (sucrose:1,6-alpha-D-glucan-6-alpha-D-glucosyltransferase, EC 2.4.1.5) from Leuconostoc mesenteroides NRRL B-512F showed that below 1 mM, Ca2+ activated the enzyme by increasing Vmax and decreasing the Km for sucrose. Above 1 mM, Ca2+ was a weak competitive inhibitor (Ki = 59 mM). Although it
A W, Miller, J F, Robyt
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Dextransucrase: Donor substrate reactions
Archives of Biochemistry and Biophysics, 1981Abstract Previous studies have indicated that α- d -1-fluoroglucose is a glycosyl donor for glucosyl transferases (5, 6) including dextransucrases formed by Leuconostoc and Streptococcus mutans. The present report confirms these observations with dextransucrase isolated from S. sanguis and conclusively establishes the details of this reaction as well
S M, Jung, R M, Mayer
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Dextransucrase: Acceptor substrate reactions
Archives of Biochemistry and Biophysics, 1981Abstract Studies were carried out on the acceptor specificity of dextransucrase which had been isolated from Streptococcus sanguis 10558. Radioactive acceptors were employed in reactions with cold sucrose and the counts incorporated were taken as a measure of “acceptor activity.” An order of relative activity was found to be polysaccharide ...
R M, Mayer +4 more
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Dextransucrase and the mechanism for dextran biosynthesis
Carbohydrate Research, 2008Remaud-Simeon and co-workers [Moulis, C.; Joucla, G.; Harrison, D.; Fabre, E.; Potocki-Veronese, G.; Monsan, P.; Remaud-Simeon, M. J. Biol. Chem., 2006, 281, 31254-31267] have recently proposed that a truncated Escherichia coli recombinant B-512F dextransucrase uses sucrose and the hydrolysis product of sucrose, D-glucose, as initiator primers for the ...
John F, Robyt +2 more
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Interaction of deoxyhalosucrose derivatives with dextransucrase
Carbohydrate Research, 1985Members of a series of deoxyhalosucrose analogs substituted at one, two, or three primary carbon atoms with bromine or chlorine were prepared. Dextransucrase isolated from Streptococcus sanguis was separately treated with 6-bromo-6-deoxysucrose, 6,6'-dibromo-6,6'-dideoxysucrose, 6,1',6'-tribromotrideoxysucrose, and 6,6'-dichlorodideoxysucrose, in order
M K, Bhattacharjee, R M, Mayer
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Production of Leucrose by Dextransucrase
Starch - Stärke, 1998Dextransucrase can be used to synthesize the disaccharide leucrose from sucrose and fructose under appropriate reaction conditions. HPLC methods to analyze the reaction solutions and an enzyme activity test were developed to determine reaction kinetics and by-products in detail. As a result, a separate acceptor binding site at the enzyme was postulated
Klaus Buchholz +2 more
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The hydrolysis of sucrose by dextransucrase
Carbohydrate Research, 1983Abstract Dextransucrase was shown to catalyze the hydrolysis of sucrose. The hydrolytic activity was found to be directly correlatable with dextransucrase activity on poly-(acrylamide) disc-gel electrophoresis. In studies on the hydrolysis of sucrose and formation of dextran as a function of time and substrate concentration, the two activities were ...
Gary A. Luzio, Robert M. Mayer
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