Results 101 to 110 of about 18,287 (222)

A Comparison Between Denaturing Gradient Gel Electrophoresis and Denaturing High Performance Liquid Chromatography in Detecting Mutations in Genes Associated with Hereditary Non-Polyposis Colorectal Cancer (HNPCC) and the Identification of 9 New Mutations Previously Unidentified by DGGE

Hereditary Cancer in Clinical Practice, 2003
Denaturing high performance liquid chromatography is a relatively new method by which heteroduplex structures formed during the PCR amplification of heterozygote samples can be rapidly identified.
Meldrum Cliff J, McPhillips Mary, Crooks Renee, Thomas Lesley, Edkins Ted, Creegan Rohanna, Miller Ewan, Agrez Michael, Scott Rodney J   +8 more
doaj   +1 more source

INVESTIGATION OF MICROBIAL COMMUNITY STRUCTURE WITH CULTURE-DEPENDENT AND INDEPENDENT PCR-DGGE METHODS IN WESTERN WEST LAKE OF HANGZHOU, CHINA

, 2012
Culture-dependent and independent PCR-DGGE methods were used to investigate the genetic diversity of microbial community in the western West Lake, Hangzhou. Water samples (n=7) were collected from the western West Lake in August, 2010.
Wu, ZB (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China., Zhou, Qiaohong, Wu, Zhenbin, Hu, Shenghua, Cai, Linlin, He, Feng, Kong, Lingwei   +6 more
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Group specific RT-PCR DGGE analysis.

, 2014
RT-PCR DGGE analysis of metabolically active free-living (0.22–8 μm in 2006 and 0.22–5 μm in 2008 and 2009) and particle-attached (>8 or >5 μm, respectively) Betaproteobacteria, Actinobacteria, and Sphingomonadaceae after 1O2 and H2O2 exposure.
Stefanie P. Glaeser (542828), Bork A. Berghoff (424746), Verena Stratmann (542829), Hans-Peter Grossart (203577), Jens Glaeser (479943)   +4 more
core   +1 more source

DGGE-RAPD analysis as a useful tool for cultivar identification

, 2016
Denaturing gradient gel electrophoresis-random amplified polymorphic DNA (DGGE-RAPD) was used to overcome the main drawbacks of RAPD (i.e., the low levels of reproducibility and polymorphism).
Mahfouz, H T, Saleh, O M, Bahieldin, A, Ahmed, I A, Gad El-Karim, Gh A, Eissa, H F   +5 more
core   +1 more source

DGGE detection of Hhal polymorphism in the F9 gene [PDF]

Nucleic Acids Research, 1990
D, Caprino, M, Acquila, M, Pecorara, P G, Mori   +3 more
openaire   +2 more sources

不同DGGE谱带信息提取方法对分析结果的影响

, 2012
自1993年Muyzer,et al.[1]将变性梯度凝胶电泳技术(Denaturing gradient gel electrophoresis,DGGE)引入到微生物生态学研究以来,DGGE已被广泛应用于各种生态系统(如淡水、海洋、土壤、动物消化道等)的微生物群落结构分析[2—6 ...
吴含含, 陈芳, 余育和, 倪加加, 颜庆云   +4 more
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The host genotype affects the bacterial community in the human gastrointestinal tract

, 2001
The gastrointestinal (GI) tract is one of the most complex ecosystems consisting of microbial and host cells. It is suggested that the host genotype, the physiology of the host and environmental factors affect the composition and function of the ...
Vos, W.M., de, Visser, J.A.G.M., de, Zoetendal, E.G., Akkermans, A.D.L., Akkermans-van Vliet, W.M.   +4 more
core   +1 more source

PCR and DGGE Detection Limits for Wine Spoilage Microbes

, 2016
In this study the culture-independent technique, polymerase chain reaction (PCR)-denaturing gradient gelelectrophoresis (DGGE), was investigated for the early detection and identification of possible spoilage microbesin wine. PCR and DGGE conditions were
M. Cameron, du Toit, M., R.C. Witthuhn, Bester, L., Cameron, M., L. Bester, Witthuhn, R.C., M. du Toit   +7 more
core   +1 more source

Effect of infant formula with probiotics on intestinal microbiota

Archivos Latinoamericanos de Nutrición
Weaning during infancy refers to the initiation of complementary food to breast milk. During weaning, there are significant changes on the gastrointestinal microbiota.
Manuel E Baldeón, Gabriela Naranjo, Diego Granja   +2 more
doaj  

Influence of Mosquito Larvae on Bacterial Species Diversity and Abundance, and on the Oviposition Response of Gravid Aedes aegypti (Diptera: Culicidae)

, 2007
The primary objectives of our study were to determine whether larval Aedes aegypti alter the bacterial community landscape of laboratory microcosms containing white oak (Quercus alba) leaf infusion (OLI) and to evaluate the degree to which a larval ...
Evans, Brian Patrick
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