Results 131 to 140 of about 10,156 (179)
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1998
The polymerase chain reaction (PCR) has become the basic tool for the amplification of genomic DNA fragments. In particular, the analysis of coding and regulatory regions are important as targets for the study of genetic diversity. To speed up methods for revealing variation in these sequences, new techniques have to be developed with the potential to ...
Michael Etscheid, Detlev Riesner
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The polymerase chain reaction (PCR) has become the basic tool for the amplification of genomic DNA fragments. In particular, the analysis of coding and regulatory regions are important as targets for the study of genetic diversity. To speed up methods for revealing variation in these sequences, new techniques have to be developed with the potential to ...
Michael Etscheid, Detlev Riesner
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Denaturing Gradient Gel Electrophoresis (DGGE)
2013Denaturing Gradient Gel Electrophoresis (DGGE) is a technique used to separate short- to medium-length DNA fragments based on their melting characteristics. It has been used frequently for identifying single-nucleotide polymorphisms without the need for DNA sequencing and as a molecular fingerprinting method for complex ecosystem communities, in ...
Fiona, Strathdee, Andrew, Free
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Modifications for the Improvement of TGGE and DGGE
1998Adding a 20–40 base-long stretch of guanosine and cytosine residues to the 5′ end of one of the PCR primers leads to a PCR fragment with one extremely thermostable end and shifts the irreversible transition during DGGE and TGGE to a higher temperature or higher denaturant concentration, respectively (9).
Michael Etscheid, Detlev Riesner
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Mutation detection by denaturing gradient gel electrophoresis (DGGE)
Human Mutation, 1994The molecular analysis of genetic diseases relies on several technical approaches which allow genetic and physical mapping, characterization of the gene structure, expression studies, and identification of disease-causing mutations. Denaturing gradient gel electrophoresis (DGGE) allows the rapid screening for single base changes in enzymatically ...
R, Fodde, M, Losekoot
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16S rRNA Targeted DGGE Fingerprinting of Microbial Communities
2008The past decades have seen the staggering development of molecular microbial ecology as a discipline that uses the detection of so-called biomarkers to monitor microbial communities in environment samples. A variety of molecules can be used as biomarkers, including cell-wall components, proteins, lipids, DNA or RNA. Especially, the application of small
Tzeneva, V.A. +5 more
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PCR–DGGE differentiation of strains of Saccharomyces sensu stricto
Antonie van Leeuwenhoek, 2004A quick molecular biology method based on the polymerase chain reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE) was developed for distinguishing strains belonging to the Saccharomyces sensu stricto group. Differentiation was obtained between S. cerevisiae, S. paradoxus and S. bayanus / S.
Marisa, Manzano +3 more
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Diversity of Conidia of Aquatic Hyphomycetes Assessed by Microscopy and by DGGE
Microbial Ecology, 2005Water samples from a Canadian stream were passed through membrane filters between 22 July 2002 and 19 May 2003. Filters with trapped conidia of aquatic hyphomycetes were cut in half. One half was examined under a light microscope, and conidia were counted and identified. From the second half, DNA was extracted and amplified with fungal primers.
N S, Raviraja +2 more
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Use of the PCR-DGGE method in forensic microbiology
Issues of Forensic Science, 2016The latest reports on the subject of forensic microbiology indicate that it is possible to link a perpetrator of an offence to the evidence, based on molecular analysis of microbiological material. The microbiome of human epidermis is known to be species- and individual-specific.
Aleksandra Ziembińska-Buczyńska +1 more
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Effect of DNA polymerases on PCR-DGGE patterns
International Biodeterioration & Biodegradation, 2013Abstract PCR-based molecular biological techniques became fundamental in the analysis of microbial communities. One of the most popular techniques is PCR-DGGE wherein the outcome is greatly influenced by the DNA amplification process. A large number of different factors were described as key elements for successful amplification, however the ...
Margit Balázs +7 more
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การวิเคราะห์ความหลากหลายของประชากรจุลินทรีย์ในถังปฏิกรณ์อีจีเอสบีโดยใช้เทคนิค PCR-DGGE
ศึกษาผลของภาระบรรทุกสารอินทรีย์และความเร็วไหลขึ้นควบคู่ไปกับ การศึกษาความหลากหลายของกลุ่มจุลินทรีย์ในถังปฏิกรณ์อีจีเอสบี โดยแบ่งการทดลองออกเป็น 3 ช่วงคือ ช่วงที่ 1 ศึกษาประสิทธิภาพของระบบอีจีเอสบีที่ภาระบรรทุกสารอินทรีย์ต่างกัน คือ 10, 20, 30 และ 40 กก.ซีโอดี/ลบ.ม.-วัน โดยใช้ความเร็วไหลขึ้น 3 และ 4 ม./ชม.openaire +1 more source