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Improvement of the inflammation-damaged intestinal barrier and modulation of the gut microbiota in ulcerative colitis after FMT in the SHIME® model. [PDF]

BMC Complement Med Ther
Kamlárová A, Kvaková M, Ambro Ľ, Link R, Bertková I, Hertelyová Z, Janíčko M, Hijová E, Štofilová J.   +8 more
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Rapid identification of Armillaria species by PCR–DGGE

Journal of Microbiological Methods, 2014
The molecular approach has been used to distinguish between Armillaria species because of the ambiguity when using morphological traits. PCR-DGGE using the fungal primers ITS3GC-ITS4 enables a selective separation of amplicons and results in a simple and rapid approach to discriminate in one-step the most common Armillaria species in Europe.
Longa, Claudia Maria Oliveira, La Porta, Nicola   +1 more
openaire   +3 more sources

TGGE and DGGE

1998
The polymerase chain reaction (PCR) has become the basic tool for the amplification of genomic DNA fragments. In particular, the analysis of coding and regulatory regions are important as targets for the study of genetic diversity. To speed up methods for revealing variation in these sequences, new techniques have to be developed with the potential to ...
Michael Etscheid, Detlev Riesner
openaire   +1 more source

Denaturing Gradient Gel Electrophoresis (DGGE)

2013
Denaturing Gradient Gel Electrophoresis (DGGE) is a technique used to separate short- to medium-length DNA fragments based on their melting characteristics. It has been used frequently for identifying single-nucleotide polymorphisms without the need for DNA sequencing and as a molecular fingerprinting method for complex ecosystem communities, in ...
Fiona, Strathdee, Andrew, Free
openaire   +2 more sources

Mutation detection by denaturing gradient gel electrophoresis (DGGE)

Human Mutation, 1994
The molecular analysis of genetic diseases relies on several technical approaches which allow genetic and physical mapping, characterization of the gene structure, expression studies, and identification of disease-causing mutations. Denaturing gradient gel electrophoresis (DGGE) allows the rapid screening for single base changes in enzymatically ...
R, Fodde, M, Losekoot
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PCR–DGGE differentiation of strains of Saccharomyces sensu stricto

Antonie van Leeuwenhoek, 2004
A quick molecular biology method based on the polymerase chain reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE) was developed for distinguishing strains belonging to the Saccharomyces sensu stricto group. Differentiation was obtained between S. cerevisiae, S. paradoxus and S. bayanus / S.
Marisa, Manzano, Luca, Cocolin, Benedetta, Longo, Giuseppe, Comi   +3 more
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Modifications for the Improvement of TGGE and DGGE

1998
Adding a 20–40 base-long stretch of guanosine and cytosine residues to the 5′ end of one of the PCR primers leads to a PCR fragment with one extremely thermostable end and shifts the irreversible transition during DGGE and TGGE to a higher temperature or higher denaturant concentration, respectively (9).
Michael Etscheid, Detlev Riesner
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Diversity of Conidia of Aquatic Hyphomycetes Assessed by Microscopy and by DGGE

Microbial Ecology, 2005
Water samples from a Canadian stream were passed through membrane filters between 22 July 2002 and 19 May 2003. Filters with trapped conidia of aquatic hyphomycetes were cut in half. One half was examined under a light microscope, and conidia were counted and identified. From the second half, DNA was extracted and amplified with fungal primers.
N S, Raviraja, L G, Nikolcheva, F, Bärlocher   +2 more
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