Results 61 to 70 of about 32,497 (210)

Ovine pedomics : the first study of the ovine foot 16S rRNA-based microbiome [PDF]

, 2011
We report the first study of the bacterial microbiome of ovine interdigital skin based on 16S rRNA by pyrosequencing and conventional cloning with Sanger-sequencing.
A Dhawi, AL Cogen, AP Escayg, Atiya Ul-Hassan, BF Langworth, BH Jost, Brian B Oakley, C Lozupone, Claire L Russell, DA Murdoch, DJ Lane, DJ Stewart, DL Emery, DS Roberts, E Pruesse, E Zaura, EA Grice, EA Grice, Elizabeth MH Wellington, G Bennett, G Conrads, G Jones, G Muyzer, G Sayers, GC Baker, GR Carter, Graham F Medley, H Ulbegi-Mohyla, H Zhou, I Demirkan, IH Davies, J Kaler, J Kaler, J Kaler, JA Frank, Jasmeet Kaler, JC Chin, JP Galkiewicz, JR Egerton, K Klitgaard, Laura E Green, LE Green, Leo A Calvo-Bado, LJ Moore, Luci Witcomb, M Chrino-Trejo, MC Liu, Miloslav Kocur, NJ Evans, NJ Evans, PA Lawson, PD Schloss, PD Schloss, RD Naylor, RE Ley, RJ Collighan, Rose Grogono-Thomas, RS Howell-Jones, S La Fontaine, S Lavín, S Piriz Duran, S Tadepalli, Scot E Dowd, SE Dowd, SJ Billington, TG Nagaraja, TM Skerman, TZ DeSantis, TZ DeSantis Jr, Vicky Bateman, W Li, W Ludwing, WE Kloos, WG Weisburg, WIB Beveridge, William Gaze   +75 more
core   +2 more sources

Ligase‐Catalyzed Transcription and Reverse Transcription of XNA by T3 DNA Ligase

Current Protocols, Volume 6, Issue 1, January 2026.
Abstract The growing utility of xeno‐nucleic acids (XNAs) lies in their ability to extend the reach of genetic chemistry beyond the limits imposed by natural polymers. XNAs, with their diverse chemical backbones, resist enzymatic degradation and yet retain the capacity for sequence‐defined information, and have found broad applications in biotechnology.
Natalie Khamissi, Luca Rustico, Ryan Hili   +2 more
wiley   +1 more source

Biodegradation of Chlorpyrifos in Paddy Rice Soil in the Mekong Delta, Vietnam

European Journal of Biology
Objective: Chlorpyrifos (CP) is an organophosphate insecticide. High toxicity makes it a potential source of soil pollution when used in agriculture.
Truong Quoc Tat
doaj   +1 more source

Mutations in the low density lipoprotein receptor gene of familial hypercholesterolemic patients detected by denaturing gradient gel electrophoresis and direct sequencing.

Journal of Lipid Research, 1995
Familial hypercholesterolemia (FH) results from mutations in the low density lipoprotein receptor (LDLR) gene. We applied denaturing gradient gel electrophoresis (DGGE) to screen for sequence variations in the coding and splice site consensus sequences ...
P Lombardi, E J Sijbrands, K van de Giessen, A H Smelt, J J Kastelein, R R Frants, L M Havekes   +6 more
doaj   +1 more source

Homing in on Endogenous Badnaviral Elements: Development of Multiplex PCR-DGGE for Detection and Rapid Identification of Badnavirus Sequences in Yam Germplasm

Frontiers in Plant Science, 2022
Viruses of the genus Badnavirus (family Caulimoviridae) are double-stranded DNA-reverse transcribing (dsDNA-RT) plant viruses and have emerged as serious pathogens of tropical and temperate crops globally.
Gonçalo Silva, Moritz Bömer, Aliyu A. Turaki, Chukwuemeka K. Nkere, Chukwuemeka K. Nkere, Chukwuemeka K. Nkere, P. Lava Kumar, Susan E. Seal   +7 more
doaj   +1 more source

Millimetre‐Scale Stratification of Microbial Communities in Hydrothermal Sediments

Environmental Microbiology, Volume 28, Issue 1, January 2026.
Zooming into the millimetre‐scale spatial distribution of organic molecules in hydrothermally impacted sediments using mass spectrometry imaging reveals a distinct lipid zonation with abrupt transitions at critical redox interfaces. This zonation reflects a pronounced stratification of diverse microbial communities and highlights surprisingly ...
Janina Groninga, Weimin Liu, Lars Wörmer, Jenny Altun, Andreas Teske, Kai‐Uwe Hinrichs   +5 more
wiley   +1 more source

The role of bacteria in pine wilt disease: insights from microbiome analysis. [PDF]

, 2018
Pine Wilt Disease (PWD) has a significant impact on Eurasia pine forests. The microbiome of the nematode (the primary cause of the disease), its insect vector, and the host tree may be relevant for the disease mechanism.
Alves, Marta, Correia, A, Henriques, Isabel, Henriques, J, Lopes, H, Matos, P, Mota, Manuel, Nascimento, F, Pereira, A, Vicente, Cláudia   +9 more
core   +1 more source

Molecular Responses to Temperature Changes Across Timescales in the Madagascar Ground Gecko (Paroedura picta)

Molecular Ecology, Volume 35, Issue 2, January 2026.
ABSTRACT Short‐term responses to temperature stress, such as heat waves and long‐term acclimation to temperature changes, including seasonal shifts, are expected to be mediated by distinct molecular pathways. However, in ectotherms, such as reptiles, the effects of exposure duration on molecular responses to temperature change remain unclear.
Fuku Sakamoto, Shunsuke Kanamori, Félix Rakotondraparany, Takashi Makino, Masakado Kawata   +4 more
wiley   +1 more source

Comparison of DNA extraction kits for PCR-DGGE analysis of human intestinal microbial communities from fecal specimens

Nutrition Journal, 2010
Background The influence of diet on intestinal microflora has been investigated mainly using conventional microbiological approaches. Although these studies have advanced knowledge on human intestinal microflora, it is imperative that new methods are ...
Nakatsu Cindy H, Savaiano Dennis A, Ariefdjohan Merlin W   +2 more
doaj   +1 more source

Comparison of bacterial communities of tilapia fish from Cameroon and Vietnam using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) [PDF]

, 2009
Fishes in general and tilapia in particular are traded all over the world. However, it is difficult to find out their exact geographical location. One of the techniques used in the traceability of fish and its byproducts consist in analysing in a global ...
Loiseau, Gérard, Maïworé, Justine, Mbofung, C.M.F., Montet, Didier, Tatsadjieu, Ngoune Léopold   +4 more
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