Rapid Plasmid-Free Generation of Recombinant Positive-Strand RNA Viruses That Use IRES-Mediated Translation Using an Expansion of the Circular Polymerase Extension Reaction (CPER). [PDF]
Reverse genetics systems in virology are technologies used to generate recombinant viruses, enabling the manipulation of viral genes. Recombinant viruses facilitate the investigation of pathogenesis and the development of antivirals.
Yamamoto H, Tamura T, Fukuhara T.
europepmc +2 more sources
Development of a one-step multiplex RT-qPCR method for rapid detection of bovine diarrhea viruses. [PDF]
IntroductionViral calf diarrhea poses a significant challenge to the cattle industry worldwide due to its high morbidity and mortality rates, leading to substantial economic losses.
Yang D+18 more
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The Influence of the Associated Inactivated Vaccine Against Infectious Rhinotracheitis and Bovine Viral Diarrhea on the Formation and Duration of Colostral Immunity in Kazakh Whiteheaded Calves. [PDF]
Objectives: This article presents a study evaluating the antibody levels against infectious bovine rhinotracheitis (IBR) and bovine viral diarrhea (BVD) in Kazakh Whiteheaded calves born to dams immunized with an experimental inactivated combined vaccine
Bulatov Y+16 more
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Primary Cells from a <i>CD46</i>-Edited Bovine Heifer Have Reduced BVDV Susceptibility Despite Viral Adaptation to Heparan Sulfate. [PDF]
A precision genome edit in the bovine CD46 gene (A82LPTFS87) dramatically reduced bovine viral diarrhea virus (BVDV) susceptibility in a cloned heifer. However, pathogen evolution threatens the long-term efficacy of such interventions.
Krueger AC+4 more
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Metagenomic insights into the complex viral composition of the enteric RNA virome in healthy and diarrheic calves from Ethiopia [PDF]
Background Viruses and the virome have received increased attention in the context of calf diarrhea and with the advancement of high-throughput sequencing the detection and discovery of viruses has been improved. Calf diarrhea, being the main contributor
Bergholm J+3 more
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Comparative Insights into Acute Gastroenteritis in Cattle Caused by Bovine Rotavirus A and Bovine Coronavirus. [PDF]
Acute gastroenteritis (AGE) in cattle significantly impacts the economy due to relatively high morbidity and mortality and decreased production. Its multifactorial nature drives its global persistence, involving enteric viruses, bacteria, protozoa, and ...
Kostanić V+5 more
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Rapid visual detection of bovine viral diarrhea virus (BVDV) using recombinase polymerase amplification with SYBR green I. [PDF]
Background Bovine diarrhea virus (BVDV) is considered to be the most common pathogen causing severe diarrhea in cattle worldwide and can cause Bovine viral diarrhea (BVD).
Jiang L+7 more
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: The bovine respiratory disease complex causes sizable losses for livestock producers. Its presentation involves the interaction among multiple viral and bacterial pathogens.
Misael Enrique Oviedo Pastrana+3 more
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In this study, the ELISA procedure was used to detect antibodies against bovine viral diarrhea (BVDV) and infectious bovine rhinotracheitis (IBRV) viruses.
Hassan Mahmoud, Alsagher Ali
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COMPARATIVE STUDY BETWEEN ROTA VIRUSES ISOLATED FROM DIARRHEATIC INFANT BABIES AND NEWLY BORN CALVES
Rota viruses were isolated in vero continuous cell line from infant babies and newly borne calves affected by diarrhea after treatment in every passage with 10 mg/ml of trypsin and adding 0.5 mg/ml of trypsin in the maintenance media.
Raja. H. Al-Falahy+2 more
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