Results 41 to 50 of about 28,017 (236)

Hierarchically Engineered Multi‐Enzyme Nanoreactors for in vitro Drug Biosynthesis and Pathway Transplantation Into Cells

Advanced Materials, EarlyView.
A hierarchically structured metal–organic framework (eMIL) is shown to host the complete six‐enzyme biosynthesis pathway for the anti‐cancer pigment violacein, reshaping reaction dynamics and multiplying in vitro yields. eMIL nanoreactors deliver this reconstituted bacterial multi‐protein system into mammalian cells, where cell‐derived substrates and ...
Ainur Sharip, Somayah S. Qutub, Manar M. Farooqui, Walaa Baslyman, Nida Khalfay, Lukman O. Alimi, Patricia Lopez Sanchez, Lingyun Zhao, Milena Chernyshevskaia, Giovanni Colombo, Niveen M. Khashab, Stefan T. Arold, Raik Grünberg   +12 more
wiley   +1 more source

In vitro amplification of BVDV field strains isolated in Argentina: effect of cell line and culture conditions [PDF]

, 2009
The aim of this work was to study the in vitro amplification of BVDV (Pestivirus, Flaviridae) field isolates from Argentina in MDBK, BoTur and BHK-21 continuous cell lines.
Boynak, Natalia, Faverin, Claudia, Leunda, Maria Rosa, Odeón, Anselmo Carlos, Vena, M. M., Zabal, O.   +5 more
core  

Immortalized sheep microglial cells are permissive to a diverse range of ruminant viruses

Veterinary Quarterly, 2017
Background: Ruminants, including sheep and goats (small ruminants), are key agricultural animals in many parts of the world. Infectious diseases, including many viral diseases, are significant problems to efficient production of ruminants. Unfortunately,
James B. Stanton, Beryl Swanson, Edith Orozco, Juan F. Muñoz-Gutiérrez, James F. Evermann, Julia F. Ridpath   +5 more
doaj   +1 more source

Targeting the PDK1/c‐Myc/SOX10 Signaling in Oligodendrocytes Alleviates Neuropathic Pain

Advanced Science, EarlyView.
This work reveals that oligodendrocyte homeostasis, mediated by PDK1, is a critical determinant of neuropathic pain (NPP) pathogenesis. Disruption of PDK1 in oligodendrocytes impairs SOX10‐dependent myelination programs through c‐Myc accumulation, leading to disrupted myelination and the pathophysiology of NPP.
Pingping Qiao, Lifang Guo, Guochao Yang, Chaoli Huang, He Wang, Jian‐Jun Yang, Guiquan Chen, Yimin Hu   +7 more
wiley   +1 more source

Genetic diversity of bovine viral diarrhea viruses in commercial bovine serum batches of Chinese origin

Infection, Genetics and Evolution, 2014
Bovine viral diarrhea virus (BVDV) is often detected in commercial bovine serum. BVDV genetic diversity was investigated in commercial bovine serum of Chinese origin. Twenty-two batches of bovine serum were obtained from 10 suppliers with different geographic origins in China, and 20 batches of bovine serum were positive by reverse-transcription ...
Shu-Qin, Zhang, Bin, Tan, Li, Guo, Feng-Xue, Wang, Hong-Wei, Zhu, Yong-Jun, Wen, Shipeng, Cheng   +6 more
openaire   +2 more sources

Visualization of Endosomal Escape of Intracellularly Delivered Protein With Unexpected Photochemical Internalization of Trypan Blue

Advanced Science, EarlyView.
TB, a conventional cell viability dye, acts as both a fluorescence modulator (quenching GFP fluorescence upon protein binding and recovering it upon release) and a photosensitizer that triggers endosomal escape via photochemical internalization (PCI) under 590 nm light.
Zhongqi Yao, Zhan Shi, Peng Hu, Changping Wang, Hui Wang   +4 more
wiley   +1 more source

Self‐Assembling Hybrid Hydrogel Reprograms the Stromal Vascular Fraction to Treat Osteoarthritis

Advanced Science, EarlyView.
This study presents a bioinspired injectable hydrogel that enhances the therapeutic potential of stem cell‐rich stromal vascular fraction for treating osteoarthritis. By reprogramming cell behavior through epigenetic modulation, the hydrogel promotes cartilage regeneration and reduces joint damage in a rat model, offering a promising new approach for ...
Waifang Hou, Yongfeng Chen, Longyou Xiao, Wei Qi, Tianshu Du, Qiang Sun, Borui Xue, Qinghe Zhao, Pengfei Xie, Zi Ye, Fei Han, Lingli Guo, Yang Jiao, Ranran Zhang, Hong Jia, Xiao Huang, Jianwei Guo, Peng Wang, Huayi Wang, Yuanrui Wang, Wenjing Chen, Haining Wu, Liumin He, Dawei Zhang   +23 more
wiley   +1 more source

Assessment of two essential elements of BVDV control on selected Flemish dairy and beef farms [PDF]

, 2013
Bovine viral diarrhea virus (BVDV) is one of the most important viruses to cause disease in cattle worldwide. The virus is endemically present in Belgium. Clinical diagnosis of BVDV infection is difficult.
Booth, Richard, De Vliegher, Sarne, Deprez, Piet, Dewulf, Jeroen, Laureyns, Jozef, Pfeiffer, Dirk, Sarrazin, Steven   +6 more
core  

Genetic Typing of Bovine Viral Diarrhea Viruses (BVDV) Circulating in Korea

Journal of Bacteriology and Virology, 2007
To characterize the genetic diversity of bovine viral diarrhea viruses (BVDV) circulating in Korea, 11 BVDV isolates were obtained from 467 field samples collected during 2005~2006 in Korea. All of the BVDV isolates were identified as non-cytopathic (non-cp) BVDV biotypes. The 5' noncoding region (NCR) genes of the isolates were sequenced and analyzed.
Dong-Kun Yang, Byoung-Han Kim, Chang-Hee Kweon, Jeong-Kyu Park, Ha-Young Kim, Byung-Jae So, In-Joong Kim   +6 more
openaire   +1 more source

Evaluation of the effectiveness of the SurePure Turbulator ultraviolet-C irradiation equipment on inactivation of different enveloped and non-enveloped viruses inoculated in commercially collected liquid animal plasma.

PLoS ONE, 2019
The objective of this study was to evaluate the effectiveness of the SurePure Turbulator ultraviolet-C (UV-C, 254 nm wavelength) irradiation equipment on inactivation of different enveloped and non-enveloped viruses in commercially collected liquid ...
Elena Blázquez, Carmen Rodríguez, Jesús Ródenas, Núria Navarro, Cristina Riquelme, Rosa Rosell, Joy Campbell, Joe Crenshaw, Joaquim Segalés, Joan Pujols, Javier Polo   +10 more
doaj   +1 more source