The Dictyostelium Centrosome [PDF]
Cells, 2021 The centrosome of Dictyostelium amoebae contains no centrioles and consists of a cylindrical layered core structure surrounded by a corona harboring microtubule-nucleating γ-tubulin complexes. It is the major centrosomal model beyond animals and yeasts.
Ralph Gräf +4 more
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Opposite functions of RapA and RapC in cell adhesion and migration in Dictyostelium
Animal Cells and Systems, 2021 There are three Rap proteins in Dictyostelium. RapA is a key regulator of cell adhesion and cytoskeletal rearrangement. Recently, RapC has been reported to be involved in cytokinesis, cell migration, and multicellular development.
Jihyeon Jeon +2 more
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Dictyostelium AMPKα regulates aggregate size and cell-type patterning [PDF]
Open Biology, 2017 Starved Dictyostelium cells aggregate into groups of nearly 105 cells. AMPK is a highly conserved serine/threonine protein kinase consisting of a catalytic and two regulatory subunits.
Ranjana Maurya +2 more
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The phenotypes of ATG9, ATG16 and ATG9/16 knock-out mutants imply autophagy-dependent and -independent functions [PDF]
Open Biology, 2015 Macroautophagy is a highly conserved intracellular bulk degradation system of all eukaryotic cells. It is governed by a large number of autophagy proteins (ATGs) and is crucial for many cellular processes.
Qiuhong Xiong +4 more
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Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis
Nucleus, 2022 Dictyostelium amoebae perform a semi-closed mitosis, in which the nuclear envelope is fenestrated at the insertion sites of the mitotic centrosomes and around the central spindle during karyokinesis.
Ulrike Schweigel +4 more
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The dictyostelium kinome--analysis of the protein kinases from a simple model organism. [PDF]
PLoS Genetics, 2006 Dictyostelium discoideum is a widely studied model organism with both unicellular and multicellular forms in its developmental cycle. The Dictyostelium genome encodes 285 predicted protein kinases, similar to the count of the much more advanced ...
Jonathan M Goldberg +6 more
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Gel purification of gDNA for next-generation sequencing applications
BioTechniques, 2022 We demonstrate that gDNA can be conveniently and efficiently isolated and purified using standard agarose gel electrophoresis, band excision and gel purification.
Supanut Utthiya +6 more
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Bacterial Survival in Dictyostelium [PDF]
BIO-PROTOCOL, 2017 We performed an assay to test the ability of different E. coli strains to survive inside amoebal cells after ingestion. In the assay we incubated bacteria together with cells of Dictyostelium discoideum for six hours. After co-incubation most of the uningested bacteria were removed by centrifugation and the remaining uningested bacteria were killed by ...
Christopher Rensing +10 more
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Dissecting the function of Atg1 complex in Dictyostelium autophagy reveals a connection with the pentose phosphate pathway enzyme transketolase [PDF]
Open Biology, 2015 The network of protein–protein interactions of the Dictyostelium discoideum autophagy pathway was investigated by yeast two-hybrid screening of the conserved autophagic proteins Atg1 and Atg8.
Ana Mesquita +5 more
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Monitoring Autophagy in Dictyostelium [PDF]
, 2013 Autophagy is an intracellular degradation mechanism essential for cell survival and maintenance of cellular homeostasis, differentiation, and development. Recent research has highlighted the impact of autophagy in neurodegenerative diseases and aging.
Mesquita A +3 more
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