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2003
Differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) is an extremely powerful method for analyzing differences in gene expression between matched tissue/cell samples. Liang and Pardee originally described the technique in 1992 in their seminal paper (1). DDRT-PCR is now firmly established as a widespread powerful and commonly
G C, Sellar +3 more
+6 more sources
Differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) is an extremely powerful method for analyzing differences in gene expression between matched tissue/cell samples. Liang and Pardee originally described the technique in 1992 in their seminal paper (1). DDRT-PCR is now firmly established as a widespread powerful and commonly
G C, Sellar +3 more
+6 more sources
Molecular Biotechnology, 1998
Characterization of regulated gene expression in eukaryotic cells is essential for studying cell growth and differentiation as well as for understanding the molecular mechanisms of diseases. Differential display was developed for such comparative studies by allowing a systematic and nonbiased screening for molecular differences at the level of mRNA ...
P, Liang, A B, Pardee
openaire +4 more sources
Characterization of regulated gene expression in eukaryotic cells is essential for studying cell growth and differentiation as well as for understanding the molecular mechanisms of diseases. Differential display was developed for such comparative studies by allowing a systematic and nonbiased screening for molecular differences at the level of mRNA ...
P, Liang, A B, Pardee
openaire +4 more sources
2005
Ordered differential display (ODD) is one of the approaches that uses systematic, rather than random, sampling of transcripts for display and thereby provides means to browse through essentially all the transcripts in the compared mRNA pools. It is specifically adapted for small amounts of starting material.
Mikhail V, Matz, Ella A, Meleshkevitch
openaire +2 more sources
Ordered differential display (ODD) is one of the approaches that uses systematic, rather than random, sampling of transcripts for display and thereby provides means to browse through essentially all the transcripts in the compared mRNA pools. It is specifically adapted for small amounts of starting material.
Mikhail V, Matz, Ella A, Meleshkevitch
openaire +2 more sources
Molecular Biotechnology, 1998
Differential display of mRNA (DD) is a technique in which mRNA species expressed by a cell population are reverse transcribed and then amplified by many separate polymerase chain reactions (PCR). PCR primers and conditions are chosen so that any given reaction yields a limited number of amplified cDNA fragments, permitting their visualization as ...
J S, Zhang +3 more
openaire +2 more sources
Differential display of mRNA (DD) is a technique in which mRNA species expressed by a cell population are reverse transcribed and then amplified by many separate polymerase chain reactions (PCR). PCR primers and conditions are chosen so that any given reaction yields a limited number of amplified cDNA fragments, permitting their visualization as ...
J S, Zhang +3 more
openaire +2 more sources