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Limitations of Differential Display
Biochemical and Biophysical Research Communications, 1998Since its original description, differential display PCR (DD-PCR) has been extensively used in attempts to identify novel genes under a variety of circumstances. Despite its widespread use, however, few novel genes of interest have been identified.
P, Ledakis, H, Tanimura, T, Fojo
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Molecular Biology Reports, 2006
Differential display (DD) is a novel PCR-based technique, very commonly used to study differentially expressed genes at the mRNA level. In this paper we report a modified version of this technique that we have used to study the differences between the mRNA population from brain tissue of adult and old rats.
Rashmi U, Pathak, M S, Kanungo
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Differential display (DD) is a novel PCR-based technique, very commonly used to study differentially expressed genes at the mRNA level. In this paper we report a modified version of this technique that we have used to study the differences between the mRNA population from brain tissue of adult and old rats.
Rashmi U, Pathak, M S, Kanungo
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Targeted cDNA differential display (TcDD)
Biomolecular Engineering, 2006Targeted cDNA differential display (TcDD) was developed to study expression of a different selected gene families especially those at low copy numbers per cell. This method is an adaptation of our previously described targeted genomic differential display method (TGDD).
Giang H, Nguyen +5 more
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Recent advances in differential display
Current Opinion in Immunology, 1995Differential display and RNA arbitrary primed polmerase chain reaction are methods recently designed to identify and isolate differentially expressed genes. Methodological modifications have since been introduced to streamline the techniques. The major effort has centered on how to eliminate false positives as approached from a variety of angles ...
P, Liang, A B, Pardee
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2015
Die „Differential-Display“ PCR (DD-PCR) ist eine Methode, die im Jahre 1992 von Liang und Pardee eingefuhrt wurde. Es handelt sich um eine Variante der RT-PCR, da aus einer mRNA PCR-Amplifikate erzeugt werden (Zhang et al. 1998). Im Gegensatz zur konventionellen RT-PCR liegen allerdings keinerlei Informationen uber die Amplifikate vor. Mit Hilfe dieser
Hans-Joachim Müller +1 more
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Die „Differential-Display“ PCR (DD-PCR) ist eine Methode, die im Jahre 1992 von Liang und Pardee eingefuhrt wurde. Es handelt sich um eine Variante der RT-PCR, da aus einer mRNA PCR-Amplifikate erzeugt werden (Zhang et al. 1998). Im Gegensatz zur konventionellen RT-PCR liegen allerdings keinerlei Informationen uber die Amplifikate vor. Mit Hilfe dieser
Hans-Joachim Müller +1 more
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Differential display and protein quantification
2007High-throughput quantitation of proteins is of essential importance for all systems biology approaches and provides complementary information on steady-state gene expression and perturbation-induced systems responses. This information is necessary because it is, e.g., difficult to predict protein concentrations from the level of mRNAs, since regulatory
Erich, Brunner +3 more
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2000
Abstract When the technique of differential display was first described in the early 1990s, sequence information existed for a few thousand human genes and some functional information was available for most of those. At about that time, the development of high throughput sequencing technologies and large genome databases, including ...
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Abstract When the technique of differential display was first described in the early 1990s, sequence information existed for a few thousand human genes and some functional information was available for most of those. At about that time, the development of high throughput sequencing technologies and large genome databases, including ...
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1997
Most biological processes rely on changes in the level and pattern of gene expression. Their molecular and functional dissection often requires the isolation and subsequent characterization of genes that are differentially expressed in space (different tissues or organs), in time (different developmental stages), or in only one sex.
George Dimopoulos, Christos Louis
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Most biological processes rely on changes in the level and pattern of gene expression. Their molecular and functional dissection often requires the isolation and subsequent characterization of genes that are differentially expressed in space (different tissues or organs), in time (different developmental stages), or in only one sex.
George Dimopoulos, Christos Louis
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