Results 101 to 110 of about 6,190 (152)

Analysis of the Thermotropic Phase Transitions of Liposomal Phosphatidylcholine Using Differential Scanning Fluorimetry

Biological and Pharmaceutical Bulletin
Liposomes are small vesicles composed of lipid bilayers, which have been widely studied and are used in drug delivery systems (DDSs). The lipid bilayers, as two-dimensional liquid crystalline structures, show different phase states, and temperature-dependent phase transitions occur as a result of the thermotropic alteration of the physicochemical ...
Aoki, Chisa, Bamba, Yui, Uchida, Mayuka, Kogure, Yuki, Sugiki, Toshihiko, Sakai-Kato, Kumiko   +5 more
openaire   +2 more sources

Label-free analytical characterization of brazzein produced with the filamentous fungus Trichoderma reesei

Frontiers in Bioengineering and Biotechnology
Brazzein, a small, pH-stable, and heat-stable sweet protein, is a promising alternative to sucrose because of its extreme sweetness and stability.
Dominik Mojzita, Nina Aro, Juha Kontturi, Martin Kögler, Emilia Nordlund, Waltteri Hosia, Marco G. Casteleijn   +6 more
doaj   +1 more source

Rapid Characterization of Human Serum Albumin Binding for Per- and Polyfluoroalkyl Substances Using Differential Scanning Fluorimetry. [PDF]

Environ Sci Technol, 2021
Jackson TW, Scheibly CM, Polera ME, Belcher SM.   +3 more
europepmc   +1 more source

Molecular mechanism of transition-state inhibitors of bacterial antibiotic efflux pumps

npj Antimicrobials and Resistance
In many Gram-negative bacteria such as Escherichia coli and Klebsiella pneumoniae, the AcrAB-TolC efflux pump is central to multidrug resistance. We report the development of BDM91531, a nanomolar pyridylpiperazine inhibitor that potentiates the activity
Clara Börnsen, Reinke T. Müller, Anais Vieira Da Cruz, Juan-Carlos Jiménez-Castellanos, Virginie Meurillon, Lorenz Brandstätter, Eszter V. Lodinsky, Mohd Athar, Attilio V. Vargiu, Ruben C. Hartkoorn, Marion Flipo, Achilleas S. Frangakis, Klaas M. Pos   +12 more
doaj   +1 more source

Differential scanning fluorimetry at the ESFRI-Instruct Core Centre Frankfurt [PDF]

Acta Crystallographica Section A Foundations of Crystallography, 2011
Y. Thielmann, M. Kohlstädt, I. von der Hocht, J. Köpke, H. Michel   +4 more
openaire   +1 more source

A novel differential scanning fluorimetry analysis of a humanized anti-cocaine mAb and its ligand binding characteristics. [PDF]

J Immunol Methods, 2020
Kirley TL, Norman AB, Wetzel HN.
europepmc   +1 more source

Characterization of deglycosylated anti-cocaine mAb by differential scanning fluorimetry and fluorescence labeling

Abstract A humanized anti-cocaine mAb (h2E2) under development for the treatment of cocaine use disorders was deglycosylated enzymatically, and analyzed by differential scanning fluorimetry (DSF) using two distinct extrinsic dyes, and by FITC fluorescent labeling, to assess domain stability changes induced by deglycosylation of the Fc ...
openaire   +2 more sources

ShiftScan: A tool for rapid analysis of high-throughput differential scanning fluorimetry data and compound prioritization. [PDF]

Protein Sci
Waterworth SC, Shenoy SR, Sharma ND, Wolcott C, Donohue DE, O'Keefe BR, Beutler JA.   +6 more
europepmc   +1 more source

Differential scanning fluorimetry followed by microscale thermophoresis and/or isothermal titration calorimetry as an efficient tool for ligand screening. [PDF]

Biophys Rev
Winiewska-Szajewska M, Poznański J.
europepmc   +1 more source

Differential Scanning Fluorimetry and Hydrogen Deuterium Exchange Mass Spectrometry to Monitor the Conformational Dynamics of NBD1 in Cystic Fibrosis. [PDF]

Methods Mol Biol, 2019
Soya N, Roldan A, Lukacs GL.
europepmc   +1 more source