Results 151 to 160 of about 3,724 (177)
Structural analysis of an oxygen-regulated diguanylate cyclase
Acta Crystallographica Section D: Biological Crystallography, 2015 Cyclic di-GMP is a bacterial second messenger that is involved in switching between motile and sessile lifestyles. Given the medical importance of biofilm formation, there has been increasing interest in understanding the synthesis and degradation of cyclic di-GMPs and their regulation in various bacterial pathogens.
Miroslaw Tarnawski +2 more
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Shigella flexneri Diguanylate Cyclases Regulate Virulence
Journal of Bacteriology, 2021The intracellular human pathogen Shigella causes dysentery, resulting in as many as one million deaths per year. Currently, there is no approved vaccine for the prevention of shigellosis, and the incidence of antimicrobial resistance among Shigella species is on the rise.
Ruchi Ojha +3 more
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Determining Diguanylate Cyclase Activity (Radioactive Assay)
Methods in Molecular Biology, 2017Bioinformatics approaches can identify sequence motifs associated with diguanylate cyclases (DGCs), but experimental demonstration of DGC enzymatic activity is often desired. This protocol describes a sensitive radioactive assay for DGC activity in which substrate and product are quickly and easily separated by thin-layer chromatography.
Barbara I Kaźmierczak
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Molecular BioSystems, 2010
Abstract Cyclic-di-GMP signaling is used by many bacteria to control biofilm formation. As biofilm formation is an important survival strategy for many bacteria, the synthesis and degradation of cyclic-di-GMP is tightly regulated by enzymes containing domains with conserved GGDEF and EAL sequence motifs, respectively.
Niu, Liu, Taemee, Pak, Elizabeth M, Boon
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Abstract Cyclic-di-GMP signaling is used by many bacteria to control biofilm formation. As biofilm formation is an important survival strategy for many bacteria, the synthesis and degradation of cyclic-di-GMP is tightly regulated by enzymes containing domains with conserved GGDEF and EAL sequence motifs, respectively.
Niu, Liu, Taemee, Pak, Elizabeth M, Boon
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Structural characterization of a putative diguanylate cyclase conserved in hyperthermophiles
Biochemical and Biophysical Research Communications, 2019C-di-GMP, bis-(3'-5')-cyclic dimeric guanosine monophosphate, is a key signaling molecule that regulates many important physiological processes in bacteria. C-di-GMP is synthesized by diguanylate cyclase (DGC) containing the homodimeric GGDEF domain. There are many uncharacterized hypothetical proteins annotated as a putative DGC in bacteria including ...
Kyung Wook Been +3 more
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Enzymatic synthesis of c-di-GMP using a thermophilic diguanylate cyclase
Analytical Biochemistry, 2009The cyclic dinucleotide c-di-GMP is a widespread bacterial messenger molecule with potential application as a therapeutic agent for treating bacterial infection. Current enzymatic synthesis of c-di-GMP using mesophilic diguanylate cyclase (DGC) proteins suffers from low production yield due to protein instability and strong product inhibition.
Feng Rao, Zhao-Xun Liang
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In Vitro Measurement of Gas-Dependent and Redox-Sensitive Diguanylate Cyclase Activity
2023Bacteria sense and respond to gaseous ligand changes in the environment to regulate a multitude of behaviors, including the production of the secondary messengers cyclic di-GMP. Gas sensing can be difficult to measure due to the high concentration of the oxygen in the atmosphere, particularly in redox-sensitive systems.
Nushrat J, Hoque +2 more
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Expression, purification and characterization of diguanylate cyclase from Rhodococcus ruber
Protein Expression and Purification, 2019Diguanylate cyclases (DGCs) were responsible for the synthesis of second messenger cyclic di-guanosine monophosphate (c-di-GMP), which were involved in various physiological activities of bacterial species. Here, a full-length DGC from Rhodococcus ruber SD3 fused with glutathione-S-transferase (GST) was expressed in E.
Sufang, Kuang +3 more
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Biotechnology Advances, 2015
The ubiquitous bacterial cyclic di-guanosine monophosphate (c-di-GMP) emerges as an important messenger for the control of many bacterial cellular functions including virulence, motility, bioluminescence, cellulose biosynthesis, adhesion, secretion, community behaviour, biofilm formation and cell differentiation. The synthesis of this cyclic nucleotide
Chris G, Whiteley, Duu-Jong, Lee
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The ubiquitous bacterial cyclic di-guanosine monophosphate (c-di-GMP) emerges as an important messenger for the control of many bacterial cellular functions including virulence, motility, bioluminescence, cellulose biosynthesis, adhesion, secretion, community behaviour, biofilm formation and cell differentiation. The synthesis of this cyclic nucleotide
Chris G, Whiteley, Duu-Jong, Lee
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Enzymatic Production of c-di-GMP Using a Thermophilic Diguanylate Cyclase
2017C-di-GMP has emerged as a prevalent bacterial messenger that controls a multitude of bacterial behaviors. Having access to milligram or gram quantities of c-di-GMP is essential for the biochemical and structural characterization of enzymes and effectors involved in c-di-GMP signaling.
Prabhadevi, Venkataramani +1 more
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