Studying GGDEF Domain in the Act: Minimize Conformational Frustration to Prevent Artefacts [PDF]
Life, 2021 GGDEF-containing proteins respond to different environmental cues to finely modulate cyclic diguanylate (c-di-GMP) levels in time and space, making the allosteric control a distinctive trait of the corresponding proteins.
F Mantoni +2 more
exaly +12 more sources
Previously Uncharacterised Aliphatic Amino Acid Positions Modulate the Apparent Catalytic Activity of the EAL Domain of ZMO_1055 and Other Cyclic Di‐GMP‐Specific EAL Phosphodiesterases [PDF]
Microbial BiotechnologyThe nearly ubiquitous second messenger cyclic di‐GMP signalling system decides about the bacterial lifestyle transition between sessility and motility. GGDEF diguanylate cyclase and EAL phosphodiesterase domains conventionally conduct the turnover of the
Lian‐Ying Cao +3 more
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Activation mechanism of a small prototypic Rec-GGDEF diguanylate cyclase [PDF]
Nature Communications, 2021 As part of two-component systems, diguanylate cyclases (DGCs) are activated by phosphorylation. Structural and computational analyses of DgcR, a model DGC, reveal the phosphorylation-induced conformational changes and the activation mechanism likely ...
Raphael D. Teixeira +2 more
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PafS Containing GGDEF-Domain Regulates Life Activities of Pseudomonas glycinae MS82
Microorganisms, 2022 Cyclic dimeric guanosine monophosphate (c-di-GMP) is synthesized by diguanylate cyclase (DGC) with the GGDEF domain. As a ubiquitous bacterial second messenger, it regulates diverse life-activity phenotypes in some bacteria.
Xianyi Chen +9 more
doaj +4 more sources
GGDEF domain as spatial on-switch for a phosphodiesterase by interaction with landmark protein HubP [PDF]
npj Biofilms and Microbiomes, 2022 In bacteria, the monopolar localization of enzymes and protein complexes can result in a bimodal distribution of enzyme activity between the dividing cells and heterogeneity of cellular behaviors.
Tim Rick +12 more
doaj +9 more sources
Expression Patterns, Genomic Conservation and Input Into Developmental Regulation of the GGDEF/EAL/HD-GYP Domain Proteins in Streptomyces [PDF]
Frontiers in Microbiology, 2018 To proliferate, antibiotic-producing Streptomyces undergo a complex developmental transition from vegetative growth to the production of aerial hyphae and spores.
Mahmoud M Al-Bassam +2 more
exaly +4 more sources
Genetic Dissection of Cyclic di‐GMP Signalling in Pseudomonas aeruginosa via Systematic Diguanylate Cyclase Disruption [PDF]
Microbial BiotechnologyThe second messenger bis‐(3′ → 5′)‐cyclic dimeric guanosine monophosphate (c‐di‐GMP) governs adaptive responses in the opportunistic pathogen Pseudomonas aeruginosa, including biofilm formation and the transition from acute to chronic infections ...
Román A. Martino +5 more
doaj +3 more sources
Horticulturae, 2023 Cyclic diguanylate (c-di-GMP) affects bacterial physiological and biochemical functions like biofilm, motility, virulence, and bacterial secretion systems. GGDEF/EAL-domain proteins, participating in c-di-GMP synthesis and degradation, are widely present
Jinsheng Lin +8 more
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eLife, 2019 A newfound signaling pathway employs a GGDEF enzyme with unique activity compared to the majority of homologs associated with bacterial cyclic di-GMP signaling.
Zachary F Hallberg +7 more
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Retrieving of diguanylate cyclases responsible for biofilm formation and antibiotic resistance in Acinetobacter baumannii [PDF]
BMC MicrobiologyBackground As a common opportunistic nosocomial pathogen, Acinetobacter baumannii has emerged as an increasingly multidrug-resistant threat due to its strong biofilm lifestyle. The GGDEF/EAL domain proteins regulate c-di-GMP metabolism, thereby affecting
Boqiao Li, Ling Zou, Xiaobo Liu
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