Studying GGDEF Domain in the Act: Minimize Conformational Frustration to Prevent Artefacts [PDF]
Life, 2021 GGDEF-containing proteins respond to different environmental cues to finely modulate cyclic diguanylate (c-di-GMP) levels in time and space, making the allosteric control a distinctive trait of the corresponding proteins.
Federico Mantoni +2 more
exaly +11 more sources
PafS Containing GGDEF-Domain Regulates Life Activities of Pseudomonas glycinae MS82
Microorganisms, 2022 Cyclic dimeric guanosine monophosphate (c-di-GMP) is synthesized by diguanylate cyclase (DGC) with the GGDEF domain. As a ubiquitous bacterial second messenger, it regulates diverse life-activity phenotypes in some bacteria.
Xianyi Chen +9 more
doaj +4 more sources
Previously Uncharacterised Aliphatic Amino Acid Positions Modulate the Apparent Catalytic Activity of the EAL Domain of ZMO_1055 and Other Cyclic Di‐GMP‐Specific EAL Phosphodiesterases [PDF]
Microbial BiotechnologyThe nearly ubiquitous second messenger cyclic di‐GMP signalling system decides about the bacterial lifestyle transition between sessility and motility. GGDEF diguanylate cyclase and EAL phosphodiesterase domains conventionally conduct the turnover of the
Lian‐Ying Cao +3 more
doaj +3 more sources
GGDEF domain as spatial on-switch for a phosphodiesterase by interaction with landmark protein HubP [PDF]
npj Biofilms and Microbiomes, 2022 In bacteria, the monopolar localization of enzymes and protein complexes can result in a bimodal distribution of enzyme activity between the dividing cells and heterogeneity of cellular behaviors.
Tim Rick +12 more
doaj +11 more sources
Diverse Biological Functions and Domain-Specific Interactions of the Diguanylate Cyclase PA2072 in Pseudomonas aeruginosa PAO1 [PDF]
International Journal of MicrobiologyNucleotide bis-(3,5)-cyclic di-guanosine monophosphate (c-di-GMP) is a universal and versatile secondary messenger. It is involved in the regulation of significant functions such as motility, biofilm formation, virulence, drug resistance, cell cycle ...
Xiaojing Gao +6 more
doaj +3 more sources
Activation mechanism of a small prototypic Rec-GGDEF diguanylate cyclase [PDF]
Nature Communications, 2021 As part of two-component systems, diguanylate cyclases (DGCs) are activated by phosphorylation. Structural and computational analyses of DgcR, a model DGC, reveal the phosphorylation-induced conformational changes and the activation mechanism likely ...
Raphael D. Teixeira +2 more
doaj +7 more sources
Archaeal Genes Code for GGDEF Domain Proteins With Diguanylate Cyclase Activity
Molecular Microbiology, EarlyView.A biofilm‐like mode of growth is hypothesized to be required for the emergence of cellular life. Occurrence of common biofilm gene products in current Bacteria and Archaea is an indication for their previous presence in the last universal common ancestor LUCA and perhaps even in the first universal common ancestor FUCA.
Roman Sidorov +6 more
wiley +2 more sources
eLife, 2019 A newfound signaling pathway employs a GGDEF enzyme with unique activity compared to the majority of homologs associated with bacterial cyclic di-GMP signaling.
Zachary F Hallberg +7 more
doaj +3 more sources
Microbiology Spectrum, 2023 Colony pattern formations of bacteria with motility manifest complicated morphological self-organization phenomena. Leptolyngbya boryana is a filamentous cyanobacterium, which has been used as a genetic model organism for studying metabolism including ...
Kazuma Toida +10 more
doaj +3 more sources
Retrieving of diguanylate cyclases responsible for biofilm formation and antibiotic resistance in Acinetobacter baumannii [PDF]
BMC MicrobiologyBackground As a common opportunistic nosocomial pathogen, Acinetobacter baumannii has emerged as an increasingly multidrug-resistant threat due to its strong biofilm lifestyle. The GGDEF/EAL domain proteins regulate c-di-GMP metabolism, thereby affecting
Boqiao Li, Ling Zou, Xiaobo Liu
doaj +2 more sources