Results 171 to 180 of about 5,655 (194)
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Molecular BioSystems, 2010
Cyclic-di-GMP signaling is used by many bacteria to control biofilm formation. As biofilm formation is an important survival strategy for many bacteria, the synthesis and degradation of cyclic-di-GMP is tightly regulated by enzymes containing domains with conserved GGDEF and EAL sequence motifs, respectively.
Niu, Liu, Taemee, Pak, Elizabeth M, Boon
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Cyclic-di-GMP signaling is used by many bacteria to control biofilm formation. As biofilm formation is an important survival strategy for many bacteria, the synthesis and degradation of cyclic-di-GMP is tightly regulated by enzymes containing domains with conserved GGDEF and EAL sequence motifs, respectively.
Niu, Liu, Taemee, Pak, Elizabeth M, Boon
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Expression, purification and characterization of diguanylate cyclase from Rhodococcus ruber
Protein Expression and Purification, 2019Diguanylate cyclases (DGCs) were responsible for the synthesis of second messenger cyclic di-guanosine monophosphate (c-di-GMP), which were involved in various physiological activities of bacterial species. Here, a full-length DGC from Rhodococcus ruber SD3 fused with glutathione-S-transferase (GST) was expressed in E.
Sufang, Kuang +3 more
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Structural characterization of a putative diguanylate cyclase conserved in hyperthermophiles
Biochemical and Biophysical Research Communications, 2019C-di-GMP, bis-(3'-5')-cyclic dimeric guanosine monophosphate, is a key signaling molecule that regulates many important physiological processes in bacteria. C-di-GMP is synthesized by diguanylate cyclase (DGC) containing the homodimeric GGDEF domain. There are many uncharacterized hypothetical proteins annotated as a putative DGC in bacteria including ...
Kyung Wook Been +3 more
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Biotechnology Advances, 2015
The ubiquitous bacterial cyclic di-guanosine monophosphate (c-di-GMP) emerges as an important messenger for the control of many bacterial cellular functions including virulence, motility, bioluminescence, cellulose biosynthesis, adhesion, secretion, community behaviour, biofilm formation and cell differentiation. The synthesis of this cyclic nucleotide
Chris G, Whiteley, Duu-Jong, Lee
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The ubiquitous bacterial cyclic di-guanosine monophosphate (c-di-GMP) emerges as an important messenger for the control of many bacterial cellular functions including virulence, motility, bioluminescence, cellulose biosynthesis, adhesion, secretion, community behaviour, biofilm formation and cell differentiation. The synthesis of this cyclic nucleotide
Chris G, Whiteley, Duu-Jong, Lee
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Determining Diguanylate Cyclase Activity (Radioactive Assay)
2017Bioinformatics approaches can identify sequence motifs associated with diguanylate cyclases (DGCs), but experimental demonstration of DGC enzymatic activity is often desired. This protocol describes a sensitive radioactive assay for DGC activity in which substrate and product are quickly and easily separated by thin-layer chromatography.
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In Vitro Measurement of Gas-Dependent and Redox-Sensitive Diguanylate Cyclase Activity
2023Bacteria sense and respond to gaseous ligand changes in the environment to regulate a multitude of behaviors, including the production of the secondary messengers cyclic di-GMP. Gas sensing can be difficult to measure due to the high concentration of the oxygen in the atmosphere, particularly in redox-sensitive systems.
Nushrat J, Hoque +2 more
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Enzymatic Production of c-di-GMP Using a Thermophilic Diguanylate Cyclase
2017C-di-GMP has emerged as a prevalent bacterial messenger that controls a multitude of bacterial behaviors. Having access to milligram or gram quantities of c-di-GMP is essential for the biochemical and structural characterization of enzymes and effectors involved in c-di-GMP signaling.
Prabhadevi, Venkataramani +1 more
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ASSESSMENT OF A PREDICTED DIGUANYLATE CYCLASE IN TREPONEMA DENTICOLA
2018VCU Theses and ...
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Intact and Degenerate Diguanylate Cyclases regulateShigellaCyclic di-GMP
AbstractThe intracellular human pathogenShigellainvades the colonic epithelium to cause disease. Prior to invasion, this bacterium navigates through different environments within the human body, including the stomach and the small intestine. To adapt to changing environments,Shigellauses the bacterial second messenger c-di-GMP signaling system ...Ruchi Ojha +3 more
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