Structural studies of the periplasmic portion of the diguanylate cyclase CdgH from Vibrio cholerae [PDF]
Scientific Reports, 2017 Cyclic diguanylate monophosphate (c-di-GMP) is a second messenger involved in bacterial signal transduction and produced by diguanylate cyclases (DGCs) generally containing highly variable periplasmic signal-recognition domains. CdgH is a DGC enzyme that
Min Xu +4 more
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Genetic Dissection of Cyclic di-GMP Signalling in Pseudomonas aeruginosa via Systematic Diguanylate Cyclase Disruption. [PDF]
Microb BiotechnolAll 32 diguanylate cyclase (DGC)‐encoding genes of P. aeruginosa PA14 were interrupted by premature STOP codons, introduced through a multiplexed cytosine base‐editor. The synthesis of c‐di‐GMP was impaired in the resulting strain lacking all 32 DGCs (strain PA14Δ32). Phenotypic analysis confirmed major behaviour changes in P. aeruginosa PA14Δ32, which
Martino RA +5 more
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c-di-GMP-related phenotypes are modulated by the interaction between a diguanylate cyclase and a polar hub protein. [PDF]
Sci Rep, 2020 Nicastro GG +10 more
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A Symphony of Cyclases: Specificity in Diguanylate Cyclase Signaling. [PDF]
Annu Rev Microbiol, 2017 Cyclic diguanylate (c-di-GMP) is a near universal signaling molecule produced by diguanylate cyclases that can direct a variety of bacterial behaviors. A major area of research over the last several years has been aimed at understanding how a cell with dozens of diguanylate cyclases can deploy a given subset of them to produce a desired phenotypic ...
Dahlstrom KM, O'Toole GA.
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Ligand-Mediated Biofilm Formation via Enhanced Physical Interaction between a Diguanylate Cyclase and Its Receptor. [PDF]
mBio, 2018 Giacalone D +5 more
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Intact and mutated Shigella diguanylate cyclases increase c-di-GMP [PDF]
Journal of Biological ChemistryThe intracellular human pathogen Shigella invades the colonic epithelium to cause disease. Prior to invasion, this bacterium navigates through different environments within the human body, including the stomach and the small intestine. To adapt to changing environments, Shigella uses the bacterial second messenger cyclic di-GMP (c di-GMP) signaling ...
Ruchi Ojha +3 more
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Studying GGDEF Domain in the Act: Minimize Conformational Frustration to Prevent Artefacts
Life, 2021 GGDEF-containing proteins respond to different environmental cues to finely modulate cyclic diguanylate (c-di-GMP) levels in time and space, making the allosteric control a distinctive trait of the corresponding proteins.
Federico Mantoni +10 more
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π-Helix controls activity of oxygen-sensing diguanylate cyclases [PDF]
Bioscience Reports, 2020 Abstract The ability of organisms to sense and adapt to oxygen levels in their environment leads to changes in cellular phenotypes, including biofilm formation and virulence. Globin coupled sensors (GCSs) are a family of heme proteins that regulate diverse functions in response to O2 levels, including modulating synthesis of cyclic ...
Johnnie A. Walker +3 more
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Identification of flgZ as a flagellar gene encoding a PilZ domain protein that regulates swimming motility and biofilm formation in Pseudomonas. [PDF]
PLoS ONE, 2014 Diguanylate cyclase and phosphodiesterase enzymatic activities control c-di-GMP levels modulating planktonic versus sessile lifestyle behavior in bacteria.
Francisco Martínez-Granero +8 more
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Structural basis of activity and allosteric control of diguanylate cyclase [PDF]
Proceedings of the National Academy of Sciences, 2004 Recent discoveries suggest that a novel second messenger, bis-(3′→5′)-cyclic di-GMP (c-diGMP), is extensively used by bacteria to control multicellular behavior. Condensation of two GTP to the dinucleotide is catalyzed by the widely distributed diguanylate cyclase (DGC or GGDEF) domain that occurs in various combinations with sensory and ...
Carmen K. M. Chan +6 more
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