Results 111 to 120 of about 217,358 (308)

Structural features of halophilicity derived from the crystal structure of dihydrofolate reductase from the Dead Sea halophilic archaeon, Haloferax volcanii [PDF]

, 1982
Background: The proteins of halophilic archaea require high salt concentrations both for stability and for activity, whereas they denature at low ionic strength. The structural basis for this phenomenon is not yet well understood.
Pieper, Ursula, Kapadia, Geeta, Mevarech, Moshe, Herzberg, Osnat   +3 more
core   +5 more sources

Unveiling the Connection: Viral Infections and Genes in dNTP Metabolism

Viruses
Deoxynucleoside triphosphates (dNTPs) are crucial for the replication and maintenance of genomic information within cells. The balance of the dNTP pool involves several cellular enzymes, including dihydrofolate reductase (DHFR), ribonucleotide reductase (
Shih-Yen Lo, Meng-Jiun Lai, Chee-Hing Yang, Hui-Chun Li   +3 more
doaj   +1 more source

Dihydrofolate reductase as a model for studies of enzyme dynamics and catalysis [version 1; referees: 2 approved]

F1000Research, 2015
Dihydrofolate reductase from Escherichia coli (ecDHFR) serves as a model system for investigating the role of protein dynamics in enzyme catalysis. We discuss calculations predicting a network of dynamic motions that is coupled to the chemical step ...
Amnon Kohen
doaj   +1 more source

Efficacy and effectiveness of the combination of sulfadoxine/pyrimethamine and a 3-day course of artesunate for the treatment of uncomplicated falciparum malaria in a refugee settlement in Zambia. [PDF]

, 2005
In the Maheba Refugee Settlement, in the clinics supported by Medecins Sans Frontieres, all children aged up to 5 years with a confirmed diagnosis of uncomplicated falciparum malaria are treated with the combination of sulfadoxine/pyrimethamine (SP) and ...
Bloland PB, Contamin H, National Malaria Control Centre, Nosten F, Peterson DS, Trape JF, Vasconcelos KF, White NJ, World Health Organisation (WHO), World Health Organisation (WHO)   +9 more
core   +2 more sources

Short report: molecular markers associated with Plasmodium falciparum resistance to sulfadoxine-pyrimethamine in the Democratic Republic of Congo. [PDF]

, 2006
Sulfadoxine-pyrimethamine (SP) is the first line antimalarial treatment in the Democratic Republic of Congo. Using polymerase chain reaction, we assessed the prevalence of mutations in the dihydrofolate reductase (dhfr) (codons 108, 51, 59) and ...
Bonnet, M, Cohuet, S, D'Alessandro, U, Guthmann, J P, Van Herp, M, Van Overmeir, C   +5 more
core   +2 more sources

In-silico and in-vitro study of hydroxythioxanthone derivatives as a potential candidate for antibacterial agents

Results in Chemistry
A molecular docking study and molecular dynamics simulation were conducted to evaluate the inhibitory activity of hydroxythioxanthones against dihydrofolate reductase (DHFR) from Staphylococcus aureus and Bacillus subtilis proteins.
Lathifah Puji Hastuti, Faris Hermawan, Muhammad Eka Prastya, Teni Ernawati, Nicky Rahmana Putra, Jumina Jumina, Shabarni Gaffar, Savira Ekawardhani   +7 more
doaj   +1 more source

Transport of proteins into mitochondria [PDF]

, 1985
Translocational intermediates of precursor proteins of ATPase F1β subunit and cytochrome c1 across mitochondrial membranes were analyzed using two different approaches, transport at low temperature and transport after binding of precursor proteins to ...
Neupert, Walter, Schleyer, Manfred
core   +1 more source

The Role of Large-Scale Motions in Catalysis by Dihydrofolate Reductase

Journal of the American Chemical Society, 2011
Dihydrofolate reductase has long been used as a model system to study the coupling of protein motions to enzymatic hydride transfer. By studying environmental effects on hydride transfer in dihydrofolate reductase (DHFR) from the cold-adapted bacterium ...
E. Loveridge, Lai-Hock Tey, Enas M. Behiry, William M. Dawson, R. Evans, Sara B.-M. Whittaker, Ulrich L. Günther, Christopher Williams, M. Crump, R. Allemann   +9 more
semanticscholar   +1 more source

E84G mutation in dihydrofolate reductase from drug resistant strains of Mycobacterium tuberculosis (Mumbai, India) leads to increased interaction with Trimethoprim

International Journal of Mycobacteriology, 2015
Background: Dihydrofolate reductase (DHFR) (dfrA gene) is an essential enzyme for cell survival and an unexplored target in Mycobacterium tuberculosis (Mtb). This study was carried out to analyze mutations in the dfrA gene amongst 20 clinical DNA samples
Archana Raju, Savita Kulkarni, M K Ray, M G.R Rajan, Mariam S Degani   +4 more
doaj   +1 more source

Multiparameter Screening on SlipChip Used for Nanoliter Protein Crystallization Combining Free Interface Diffusion and Microbatch Methods [PDF]

, 2010
This paper describes two SlipChip-based approaches to protein crystallization: a SlipChip-based free interface diffusion (FID) method and a SlipChip-based composite method that simultaneously performs microbatch and FID crystallization methods in a ...
Du, Wenbin, Ismagilov, Rustem F., Li, Liang   +2 more
core   +1 more source