Structural complex of sterol 14α-demethylase (CYP51) with 14α-methylenecyclopropyl-Δ7-24, 25-dihydrolanosterol [PDF]
Journal of Lipid Research, 2012 Sterol 14α-demethylase (CYP51) that catalyzes the removal of the 14α-methyl group from the sterol nucleus is an essential enzyme in sterol biosynthesis, a primary target for clinical and agricultural antifungal azoles and an emerging target for ...
Galina I. Lepesheva +2 more
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Knockouts of CYP51A1, DHCR24, or SC5D from cholesterol synthesis reveal pathways modulated by sterol intermediates [PDF]
iScienceSummary: Sterols from cholesterol synthesis are crucial for cholesterol production, but also have individual roles difficult to assess in vivo due to essentiality of cholesterol.
Cene Skubic +9 more
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A low-toxicity method for the separation of lanosterol and dihydrolanosterol from commercial mixtures [PDF]
Steroids, 2004 We describe an inexpensive, low-toxicity and high-yielding method for the production of pure lanosterol and dihydrolanosterol from the commercially available mixture. Optimum conditions are presented for the one-pot production of the intermediate 24,25 vicinal diol of lanosterol acetate (via either epoxidation or hydroxyhalogenation) which is readily ...
Merilyn Manley-Harris
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Inhibitory effect of 15-oxygenated sterols on cholesterol synthesis from 24,25-dihydrolanosterol. [PDF]
Journal of Biochemistry, 1986 Several 15-oxygenated sterols were examined as to their inhibitory activity toward cholesterol synthesis from [24,25-3H]-24,25-dihydrolanosterol in the 10,000 X g supernatant fraction of a rat liver homogenate. At 40 microM, three 15 alpha-hydroxylated compounds, 14 alpha-ethylcholest-7-ene-3 beta,15 alpha-diol, 14 alpha-methylcholest-7-ene-3 beta,15 ...
Yoshiko Sonoda +2 more
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Integrated Analysis of Gut Microbiome and Lipid Metabolism in Mice Infected with Carbapenem-Resistant Enterobacteriaceae [PDF]
Metabolites, 2022 The disturbance in gut microbiota composition and metabolism has been implicated in the process of pathogenic bacteria infection. However, the characteristics of the microbiota and the metabolic interaction of commensals–host during pathogen invasion ...
Ning Zhang +10 more
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Simplified LC-MS Method for Analysis of Sterols in Biological Samples [PDF]
Molecules, 2020 We developed a simple and robust liquid chromatographic/mass spectrometric method (LC-MS) for the quantitative analysis of 10 sterols from the late part of cholesterol synthesis (zymosterol, dehydrolathosterol, 7-dehydrodesmosterol, desmosterol ...
Cene Skubic +3 more
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Journal of Lipid Research, 1981 A simplified method is described for the preparation of 14 alpha-hydroxymethyl-4,4-dimethyl-5 alpha-cholest-8-en-e beta-ol, 14 alpha-hydroxymethyl-4,4-dimethyl-5 alpha-cholest-7-en-3 beta-ol, and 14 alpha-hydroxymethyl-4,4-dimethyl-5-alpha-cholest-6-en-3
E J Parish, G J Schroepfer, Jr
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Hydroxyzine Effects on Post-Lanosterol Biosynthesis in Smith–Lemli–Opitz Syndrome (SLOS) Models [PDF]
BiomoleculesSmith–Lemli–Opitz syndrome (SLOS) is a developmental disability arising from bi-allelic pathogenic variants in the 7-dehydrocholestrol reductase (DHCR7) enzyme and the accumulation of 7-dehydrocholesterol (7-DHC).
Zeljka Korade +5 more
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Inhibition of cholesterol biosynthesis by carbon monoxide: accumulation of lanosterol and 24,25-dihydrolanosterol [PDF]
Biochemical Journal, 1972 Geoffrey F Gibbons
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Identification of 24-methylene-24,25-dihydrolanosterol as a precursor of ergosterol in the yeasts Schizosaccharomyces pombe and Schizosaccharomyces octosporus. [PDF]
FEMS Microbiology Letters, 1995 R Bonaly, J Coulon
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