Results 201 to 210 of about 246,136 (236)

Protocol for engineering poly(ethylene terephthalate) hydrolases via directed evolution using a high-throughput screening assay. [PDF]

STAR Protoc
Groseclose TM, Taylor ZK, Lujan LA, Dale T, Nguyen HB.   +4 more
europepmc   +1 more source

Directed evolution and characterisation of light harvesting complexes with altered energy transfer dynamics in purple non-sulfur bacteria

Nery ET, Avignone-Rossa C, Sapienza L, McFadden J, Olaya-Castro A, Jiménez JI.   +5 more
europepmc   +1 more source

Growth-coupled continuous directed evolution by MutaT7 enables efficient and automated enzyme engineering. [PDF]

Appl Environ Microbiol
Deng Y, Etheridge K, Ran X, Maurais HE, Sarpeshkar R.   +4 more
europepmc   +1 more source

Immune counter-evolution: immortalized B cell clones can undergo ex vivo directed evolution to counteract viral escape. [PDF]

Front Immunol
Marsman C, Heinen J, Clerico Mosina V, Maijoor K, Bakker AQ, Koslowski M, Villa A, Gullà S.   +7 more
europepmc   +1 more source

Catalytic Enantioselective Smiles Rearrangement Enabled by the Directed Evolution of P450 Radical Aryl Migratases. [PDF]

J Am Chem Soc
Fu W, Murcek K, Chen J, Liu A, Zhao Y, Liu P, Yang Y.   +6 more
europepmc   +1 more source

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Directing evolution

Proceedings of the Genetic and Evolutionary Computation Conference, 2021
As computing power grows, the automated specialization and design of evolutionary algorithms (EAs) to tune their performance to individual problem classes becomes more attractive. To this end, a significant amount of research has been conducted in recent decades, utilizing a wide range of techniques.
Braden Tisdale, Deacon Seals, Aaron Scott Pope, Daniel R. Tauritz   +3 more
openaire   +1 more source

Directed enzyme evolution

Current Opinion in Biotechnology, 2001
Laboratory evolutionists continue to generate better enzymes for industrial and research applications. Exciting developments include new biocatalysts for enantioselective carbon-carbon bond formation and fatty acid production in plants. Creative contributions to the repertoire of evolutionary methods will ensure further growth in applications and ...
Farinas, Edgardo T., Bulter, Thomas, Arnold, Frances H.   +2 more
openaire   +3 more sources

Directed Molecular Evolution

Origins of life and evolution of the biosphere, 1999
We propose the existence of a relationship of stereochemical complementarity between gene sequences that code for interacting components: nucleic acid-nucleic acid, protein-protein and protein-nucleic acid. Such a relationship would impose evolutionary constraints on the DNA sequences themselves, thus retaining these sequences and governing the ...
L F, Harris, M R, Sullivan, D L, Hatfield   +2 more
openaire   +2 more sources

Directed evolution: selecting today's biocatalysts

Biomolecular Engineering, 2005
Directed evolution has become a full-grown tool in molecular biology nowadays. The methods that are involved in creating a mutant library are extensive and can be divided into several categories according to their basic ideas. Furthermore, both screening and selection can be used to target the enzyme towards the desired direction.
Otten, Linda, Quax, Wim
openaire   +2 more sources

Directed Evolution: Methodologies and Applications

Chemical Reviews, 2021
Directed evolution aims to expedite the natural evolution process of biological molecules and systems in a test tube through iterative rounds of gene diversifications and library screening/selection. It has become one of the most powerful and widespread tools for engineering improved or novel functions in proteins, metabolic pathways, and even whole ...
Yajie Wang, Pu Xue, Mingfeng Cao, Tianhao Yu, Stephan T. Lane, Huimin Zhao   +5 more
openaire   +2 more sources