Results 241 to 250 of about 286,859 (297)

Directed evolution of LaccID for cell surface proximity labeling and electron microscopy. [PDF]

Nat Chem Biol
Lee SY, Roh H, Gonzalez-Perez D, Mackey MR, Hoces D, McLaughlin CN, Lin C, Adams SR, Nguyen K, Kim KY, Luginbuhl DJ, Luo L, Udeshi ND, Carr SA, Hernández-López RA, Ellisman MH, Alcalde M, Ting AY.   +17 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Directed Evolution of AtMP2 Peptide: Unlocking Enhanced Antibacterial Potential from <i>Anabas testudineus</i>. [PDF]

Molecules
Lee LT, Ang A, Najm A, Adnan AM, Nordin AM, Mahmood I, Dunkhorol S, Fazry S, Law D.   +8 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

A Golden Gate compatible system for continuous directed evolution in <i>E. coli</i>. [PDF]

Synth Biol (Oxf)
Sparrow Muñoz I, Burgess SJ.
europepmc   +1 more source

Directed evolution of phages in biofilms enhances Pseudomonas aeruginosa control through improved lipopolysaccharide recognition. [PDF]

Nat Commun
Meneses L, Valentová L, Santos SB, Erdmann J, Crabbé A, Plevka P, Häussler S, Pires DP, Coenye T, Azeredo J.   +9 more
europepmc   +1 more source

Directed evolution of phage Romulus in biofilm-embedded <i>Staphylococcus aureus</i>: mutations in baseplate proteins enhanced its antibiofilm activity. [PDF]

Biofilm
Campobasso C, Henderix P, Jalomo-Khayrova E, Bolognini S, Bange G, Lavigne R, Tavanti A, Wagemans J, Di Luca M.   +8 more
europepmc   +1 more source

Nonheme Fe Enzyme-Catalyzed Enantiodivergent Nitrogen Migration: Directed Evolution and Computational Study of Isopenicillin N Synthases for Biocatalytic Synthesis of Arylglycines. [PDF]

Angew Chem Int Ed Engl
Lin K, Zhao LP, Wang S, Liu H, Zhang Y, Mai BK, Liu P, Yang Y.   +7 more
europepmc   +1 more source

Directed evolution of aminoacyl-tRNA synthetases through in vivo hypermutation. [PDF]

Nat Commun
Furuhata Y, Rix G, Van Deventer JA, Liu CC.   +3 more
europepmc   +1 more source

Directed evolution of biocatalysts

Current Opinion in Chemical Biology, 1999
Directed evolution is being used increasingly in academic and industrial laboratories to modify and improve important biocatalysts. Significant advances during this period of review include compartmentalization of genes and the in vitro translation apparatus in emulsions, as well as several impressive demonstrations of catalyst improvement.
Arnold, Frances H., Volkov, Alexander A.
openaire   +4 more sources