Results 11 to 20 of about 489,553 (296)

Optimal Evolutionary Control for Artificial Selection on Molecular Phenotypes

Physical Review X, 2021
Controlling an evolving population is an important task in modern molecular genetics, including directed evolution for improving the activity of molecules and enzymes, in breeding experiments in animals and in plants, and in devising public health ...
Armita Nourmohammad, Ceyhun Eksin
doaj   +1 more source

Directed molecular evolution of ADP-glucose pyrophosphorylase [PDF]

Proceedings of the National Academy of Sciences, 2002
ADP-glucose pyrophosphorylase catalyzes a rate-limiting reaction in prokaryotic glycogen and plant starch biosynthesis. Despite sharing similar molecular size and catalytic and allosteric regulatory properties, the prokaryotic and higher plant enzymes differ in higher-order protein structure.
Peter R, Salamone, I Halil, Kavakli, Casey J, Slattery, Thomas W, Okita   +3 more
openaire   +2 more sources

Improved catalytic performance and molecular insight for lipoxygenase from Enterovibrio norvegicus via directed evolution

Frontiers in Bioengineering and Biotechnology, 2023
Lipoxygenase (LOX) holds significant promise for food and pharmaceutical industries. However, albeit its application has been hampered by low catalytic activity and suboptimal thermostability.
Bingjie Zhang, Huibing Chi, Juan Shen, Yang Tao, Zhaoxin Lu, Fengxia Lu, Ping Zhu   +6 more
doaj   +1 more source

Directed evolution of the rRNA methylating enzyme Cfr reveals molecular basis of antibiotic resistance

eLife, 2022
Alteration of antibiotic binding sites through modification of ribosomal RNA (rRNA) is a common form of resistance to ribosome-targeting antibiotics. The rRNA-modifying enzyme Cfr methylates an adenosine nucleotide within the peptidyl transferase center,
Kaitlyn Tsai, Vanja Stojković, Lianet Noda-Garcia, Iris D Young, Alexander G Myasnikov, Jordan Kleinman, Ali Palla, Stephen N Floor, Adam Frost, James S Fraser, Dan S Tawfik, Danica Galonić Fujimori   +11 more
doaj   +1 more source

Enhanced thermal and alkaline stability of L-lysine decarboxylase CadA by combining directed evolution and computation-guided virtual screening

Bioresources and Bioprocessing, 2022
As an important monomer for bio-based nylons PA5X, cadaverine is mainly produced by enzymatic decarboxylation of L-lysine. A key issue with this process is the instability of L-lysine decarboxylase (CadA) during the reaction due to the dissociation of ...
Yang Xi, Lidan Ye, Hongwei Yu
doaj   +1 more source

Prediction of designer-recombinases for DNA editing with generative deep learning

Nature Communications, 2022
Design of recombinases with new target sites is usually achieved through cycles of directed molecular evolution. Here the authors report Recombinase Generator, RecGen, an algorithm for generation of designer-recombinases; they perform experimental ...
Lukas Theo Schmitt, Maciej Paszkowski-Rogacz, Florian Jug, Frank Buchholz   +3 more
doaj   +1 more source

Deep sequencing revealed molecular signature of horizontal gene transfer of plant like transcripts in the mosquito Anopheles culicifacies: an evolutionary puzzle [version 1; referees: 2 approved]

F1000Research, 2015
In prokaryotes, horizontal gene transfer (HGT) has been regarded as an important evolutionary drive to acquire and retain beneficial genes for their survival in diverse ecologies.
Punita Sharma, Tanwee Das De, Swati Sharma, Ashwani Kumar Mishra, Tina Thomas, Sonia Verma, Vandana Kumari, Suman Lata, Namita Singh, Neena Valecha, Kailash Chand Pandey, Rajnikant Dixit   +11 more
doaj   +1 more source

Known Evolutionary Paths Are Accessible to Engineered ß-Lactamases Having Altered Protein Motions at the Timescale of Catalytic Turnover

Frontiers in Molecular Biosciences, 2020
The evolution of new protein functions is dependent upon inherent biophysical features of proteins. Whereas, it has been shown that changes in protein dynamics can occur in the course of directed molecular evolution trajectories and contribute to new ...
Lorea Alejaldre, Lorea Alejaldre, Lorea Alejaldre, Claudèle Lemay-St-Denis, Claudèle Lemay-St-Denis, Claudèle Lemay-St-Denis, Carles Perez Lopez, Ferran Sancho Jodar, Victor Guallar, Victor Guallar, Joelle N. Pelletier, Joelle N. Pelletier, Joelle N. Pelletier, Joelle N. Pelletier   +13 more
doaj   +1 more source

Improved Bioproduction of the Nylon 12 Monomer by Combining the Directed Evolution of P450 and Enhancing Heme Synthesis

Molecules, 2023
The nylon 12 (PA12) monomer ω-aminododecanoic acid (ω-AmDDA) could be synthesized from lauric acid (DDA) through multi-enzyme cascade transformation using engineered E.
Jiaming Yu, Jiawei Ge, Hongwei Yu, Lidan Ye   +3 more
doaj   +1 more source

Walks of molecular motors in two and three dimensions [PDF]

, 2002
Molecular motors interacting with cytoskeletal filaments undergo peculiar random walks consisting of alternating sequences of directed movements along the filaments and diffusive motion in the surrounding solution.
Adam G., Ajdari A., Berg H. C., Guthold M., Howard J., Kreis T., Luby-Phelps K., Nieuwenhuizen Th. M., R Lipowsky, Reimann P., S Klumpp, T. M Nieuwenhuizen, Weiss G. H.   +12 more
core   +3 more sources