Results 11 to 20 of about 495,896 (276)
Molecular Modeling in Enzyme Design, Toward In Silico Guided Directed Evolution [PDF]
, 2017 Directed evolution (DE) creates diversity in subsequent rounds of mutagenesis in the quest of increased protein stability, substrate binding, and catalysis.
Acebes, Sandra +3 more
core +4 more sources
Food Technology and Biotechnology, 2018 L-Cysteine sulfinate decarboxylase (CSD, EC 4.1.1.29), the rate-limiting enzyme in taurine synthesis pathway, catalyzes L-cysteine sulfinic acid to form hypotaurine.
Jie Deng +6 more
doaj +3 more sources
Rapid directed molecular evolution of fluorescent proteins in mammalian cells. [PDF]
Protein Sci, 2022 AbstractIn vivoimaging of model organisms is heavily reliant on fluorescent proteins with high intracellular brightness. Here we describe a practical method for rapid optimization of fluorescent proteins via directed molecular evolution in cultured mammalian cells.
Babakhanova S +18 more
europepmc +6 more sources
Directed Evolution in Drops: Molecular Aspects and Applications [PDF]
ACS Synthetic Biology, 2021 The process of optimizing the properties of biological molecules is paramount for many industrial and medical applications. Directed evolution is a powerful technique for modifying and improving biomolecules such as proteins or nucleic acids (DNA or RNA).
Manteca, Aitor +7 more
openaire +3 more sources
Directed molecular evolution to design advanced red fluorescent proteins. [PDF]
Nat Methods, 2011 Fluorescent proteins have become indispensable imaging tools for biomedical research. Continuing progress in fluorescence imaging, however, requires probes with additional colors and properties optimized for emerging techniques. Here we summarize strategies for development of red-shifted fluorescent proteins.
Subach FV, Piatkevich KD, Verkhusha VV.
europepmc +4 more sources
Directed molecular evolution by somatic hypermutation [PDF]
Protein Engineering Design and Selection, 2004 After rearrangement of immunoglobulin gene segments, the immune system evolves the antibody repertoire by mutating the immunoglobulin variable region at a high rate. While this somatic hypermutation was thought to occur only at the variable region, recent studies suggest that hypermutation can occur at locations throughout the genome.
Clifford L, Wang +2 more
openaire +2 more sources
Directed molecular evolution of ADP-glucose pyrophosphorylase [PDF]
Proceedings of the National Academy of Sciences, 2002 ADP-glucose pyrophosphorylase catalyzes a rate-limiting reaction in prokaryotic glycogen and plant starch biosynthesis. Despite sharing similar molecular size and catalytic and allosteric regulatory properties, the prokaryotic and higher plant enzymes differ in higher-order protein structure.
Peter R, Salamone +3 more
openaire +2 more sources
Directed molecular evolution reveals Gaussia luciferase variants with enhanced light output stability. [PDF]
Anal Chem, 2013 Gaussia Luciferase (Gluc) has proven to be a powerful mammalian cell reporter for monitoring numerous biological processes in immunology, virology, oncology, and neuroscience. Current limitations of Gluc as a reporter include its emission of blue light, which is absorbed by mammalian tissues, limiting its use in vivo, and a flash-type bioluminescence ...
Degeling MH +7 more
europepmc +6 more sources
Molecules, 2023 The nylon 12 (PA12) monomer ω-aminododecanoic acid (ω-AmDDA) could be synthesized from lauric acid (DDA) through multi-enzyme cascade transformation using engineered E.
Jiaming Yu +3 more
doaj +1 more source
Walks of molecular motors in two and three dimensions [PDF]
, 2002 Molecular motors interacting with cytoskeletal filaments undergo peculiar random walks consisting of alternating sequences of directed movements along the filaments and diffusive motion in the surrounding solution.
Adam G. +12 more
core +3 more sources