Results 41 to 50 of about 495,896 (276)

Evolutionary Approaches to Study Cytochrome c Peroxidase

CHIMIA, 2001
Directed molecular evolution of enzymes and proteins has emerged as an extremely powerful method to create proteins with novel properties, both for practical applications as well as for mechanistic studies.
André Iffland, Petra Tafelmeyer, Susanne Gendreizig, Kai Johnsson   +3 more
doaj   +2 more sources

Recent progress in directed evolution of stereoselective monoamine oxidases

Bioresources and Bioprocessing, 2019
Monoamine oxidases (MAOs) use molecular dioxygen as oxidant to catalyze the oxidation of amines to imines. This type of enzyme can be employed for the synthesis of primary, secondary, and tertiary amines by an appropriate deracemization protocol ...
Jiaqi Duan, Beibei Li, Youcai Qin, Yijie Dong, Jie Ren, Guangyue Li   +5 more
doaj   +1 more source

Comparative Analysis of Bacteriophytochrome Agp2 and Its Engineered Photoactivatable NIR Fluorescent Proteins PAiRFP1 and PAiRFP2

Biomolecules, 2020
Two photoactivatable near infrared fluorescent proteins (NIR FPs) named “PAiRFP1” and “PAiRFP2” are formed by directed molecular evolution from Agp2, a bathy bacteriophytochrome of Agrobacterium tumefaciens C58.
Faez Iqbal Khan, Fakhrul Hassan, Razique Anwer, Feng Juan, Dakun Lai   +4 more
doaj   +1 more source

Molecular dynamics simulation of an entire cell

Frontiers in Chemistry, 2023
The ultimate microscope, directed at a cell, would reveal the dynamics of all the cell’s components with atomic resolution. In contrast to their real-world counterparts, computational microscopes are currently on the brink of meeting this challenge.
Jan A. Stevens, Fabian Grünewald, P. A. Marco van Tilburg, Melanie König, Benjamin R. Gilbert, Troy A. Brier, Zane R. Thornburg, Zaida Luthey-Schulten, Siewert J. Marrink   +8 more
doaj   +1 more source

Expanded molecular diversity generation during directed evolution by trinucleotide exchange (TriNEx) [PDF]

Nucleic Acids Research, 2008
Trinucleotide exchange (TriNEx) is a method for generating novel molecular diversity during directed evolution by random substitution of one contiguous trinucleotide sequence for another. Single trinucleotide sequences were deleted at random positions in a target gene using the engineered transposon MuDel that were subsequently replaced with a ...
Baldwin, Amy Joy, Busse, K., Simm, A. M., Jones, Darran Dafydd   +3 more
openaire   +3 more sources

Molecular dynamic simulation on temperature evolution of SiC under directional microwave radiation

Journal of Physics: Condensed Matter, 2022
Abstract Silicon carbide (SiC) is widely used as the substrate for high power electronic devices as well as susceptors for microwave (MW) heating. The dynamics of microwave interaction with SiC is not fully understood, especially at the material boundaries.
Khan, TA, Burr, PA, Payne, D, Juhl, M, Das, U, Hallam, B, Bagnall, D, Puthen Veettil, B   +7 more
openaire   +3 more sources

The Unicellular State as a Point Source in a Quantum Biological System. [PDF]

, 2016
A point source is the central and most important point or place for any group of cohering phenomena. Evolutionary development presumes that biological processes are sequentially linked, but neither directed from, nor centralized within, any specific ...
Miller, William B, Torday, John S
core   +3 more sources

Directed Evolution and High-level Expression of α-Amylase from Thermomyces dupontii

Shipin gongye ke-ji, 2022
Objective: The α-amylase from Thermomyces dupontii was modified by direct evolution to improve its thermostability and expression level. Methods: A mutation library of α-amylase (Td-amy) from Thermomyces dupontii was constructed by error-prone PCR.
Yan JIN, Yanxiao LI, Junwen MA, Yuchuan WANG, Qiaojuan YAN, Zhengqiang JIANG   +5 more
doaj   +1 more source

Consensus mutagenesis reveals that non-helical regions influence thermal stability of horseradish peroxidase [PDF]

, 2008
The enzyme horseradish peroxidase has many uses in biotechnology but a stabilized derivative would have even wider applicability. To enhance thermal stability, we applied consensus mutagenesis (used successfully with other proteins) to recombinant ...
Amin, Arnold, Azevedo, Barry J. Ryan, Bonnaffe, Carlsson, Ciarán Ó'Fágáin, Dunford, Duroux, Eijsink, Eremin, Fishman, Flores, French, Gajhede, Garcia, Gonera, Grigorenko, Ignatenko, Kamal, Kamal, Kuhlmeyer, Lehmann, Lehmann, Lei, Lesk, Lin, Lipovsek, Liu, Magliery, Maniatis, Mary J. O'Connell, McEldoon, Miland, Miland, Miyazaki, Morawski, Nielsen, O'Brien, O'Brien, Pina, Ryan, Ryan, Ryan, Ryan, Ryan, Smith, Steipe, Tams, Thompson, van den Burg, Veitch, Wang, Watanabe, Welinder, Yang, Yang   +56 more
core   +1 more source

Pazopanib Combined With Vincristine and Irinotecan in Relapsed Wilms Tumor: Encouraging Outcomes in a Heavily Pretreated Pediatric Cohort

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background While Wilms tumor (WT) typically has a favorable prognosis, relapsed cases—especially those with high‐risk histology—remain therapeutically challenging after intensive frontline therapy. The combination of vincristine and irinotecan has demonstrated activity in pediatric solid tumors, and pazopanib, a multi‐targeted tyrosine kinase ...
Maria Debora De Pasquale, Alessandro Crocoli, Giuseppe Maria Milano, Pierluigi Di Paolo, Francesca Diomedi Camassei, Ida Russo, Annalisa Serra   +6 more
wiley   +1 more source