Results 51 to 60 of about 489,553 (296)

In-vitro engineering of novel bioactivity in the natural enzymes

Frontiers in Chemistry, 2016
Enzymes catalyze various biochemical functions with high efficiency and specificity. In-vitro design of the enzyme leads to novel bioactivity in this natural biomolecule that give answers of some vital questions like crucial residues in binding with ...
Vishvanath Tiwari
doaj   +1 more source

Directed Evolution and High-level Expression of α-Amylase from Thermomyces dupontii

Shipin gongye ke-ji, 2022
Objective: The α-amylase from Thermomyces dupontii was modified by direct evolution to improve its thermostability and expression level. Methods: A mutation library of α-amylase (Td-amy) from Thermomyces dupontii was constructed by error-prone PCR.
Yan JIN, Yanxiao LI, Junwen MA, Yuchuan WANG, Qiaojuan YAN, Zhengqiang JIANG   +5 more
doaj   +1 more source

HD101584: Circumstellar characteristics and evolutionary status

, 2019
We have performed a study of the characteristics of the circumstellar environment of the binary object HD101584, that provides information on a likely evolutionary scenario.
Bergman, P., Doan, L., Humphreys, E. M. L., Khouri, T., Lindqvist, M., Maercker, M., Nyman, L., Olofsson, H., Ramstedt, S., Tafoya, D., Vlemmings, W. H. T.   +10 more
core   +1 more source

Cold Adaptation of a Mesophilic Subtilisin-like Protease by Laboratory Evolution [PDF]

, 2000
Enzymes isolated from organisms native to cold environments generally exhibit higher catalytic efficiency at low temperatures and greater thermosensitivity than their mesophilic counterparts.
Arnold, Frances H., Miyazaki, Kentaro, Wintrode, Patrick L.   +2 more
core   +1 more source

Carcinomas and Carcinoid Tumors of the Lungs and Bronchi in Children and Adolescents: The EXPeRT Recommendations

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Primary lung carcinomas and bronchial carcinoid tumors (BC) are very rare malignancies in childhood. While typical BC and mucoepidermoid carcinomas are mostly low‐grade, localized tumors with a more favorable prognosis than in adults, necessitating avoidance of overtreatment, adenocarcinomas of the lung are often diagnosed at advanced disease ...
Michael Abele, Calogero Virgone, Michaela Kuhlen, Sabine Irtan, Yves Reguerre, Malgorzata Krawczyk, Ewa Bien, Tal Ben‐Ami, Maja Cesen, Ricardo Lopez‐Almaraz, Andrea Ferrari, Gianni Bisogno, Dominik T. Schneider, Abbas Agaimy, Constantin Lapa, Hubert Ducou le Pointe, Rainer Claus, Catherine Daniel, Ines B. Brecht, Daniel Orbach   +19 more
wiley   +1 more source

Quantification of evolved DNA-editing enzymes at scale with DEQSeq

Genome Biology, 2023
We introduce DEQSeq, a nanopore sequencing approach that rationalizes the selection of favorable genome editing enzymes from directed molecular evolution experiments.
Lukas Theo Schmitt, Aksana Schneider, Jonas Posorski, Felix Lansing, Milica Jelicic, Manavi Jain, Shady Sayed, Frank Buchholz, Duran Sürün   +8 more
doaj   +1 more source

Transposon-Based Approaches for Generating Novel Molecular Diversity During Directed Evolution [PDF]

, 2014
This chapter introduces a set of transposon-based methods that were developed to sample trinucleotide deletion, trinucleotide replacement, and domain insertion. Each approach has a common initial step that utilizes an engineered version of the Mu transposon called MuDel.
Jones, Darran Dafydd, Arpino, James A. J., Baldwin, Amy Joy, Edmundson, Matthew C.   +3 more
openaire   +3 more sources

European Standard Clinical Practice Guideline and EXPeRT Recommendations for the Diagnosis and Management of Gastroenteropancreatic Neuroendocrine Neoplasms in Children and Adolescents

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Pediatric gastroenteropancreatic neuroendocrine neoplasms (GEP‐NENs) are extremely rare and clinically heterogeneous. Management has largely been extrapolated from adult practice. This European Standard Clinical Practice Guideline (ESCP), developed by the EXPeRT network in collaboration with adult NEN experts, provides (adult) evidence ...
Michaela Kuhlen, Calogero Virgone, Antje Redlich, Michael Abele, Ricardo Lopez‐Almaraz, Tal Ben‐Ami, Ewa Bien, Gianni Bisogno, Maja Mazic Cesen, Andrea Ferrari, Malgorzata A. Krawczyk, Yves Reguerre, Dominik T. Schneider, Ines B. Brecht, Charlotte Rigaud, Bilgehan Yalcin, Rainer Claus, Christian Vokuhl, Constantin Lapa, Jörg Fuchs, Segolene Hescot, Thomas Walter, Julien Hadoux, Daniel Orbach   +23 more
wiley   +1 more source

Directed Flow in Microscopic Models in Relativistic A+A Collisions

Universe, 2019
Evolution of directed flow of charged particles produced in relativistic heavy-ion collisions at energies 4 ≤ s ≤ 19.6 GeV is considered within two microscopic transport models, ultra-relativistic quantum molecular dynamics (UrQMD) and ...
Larissa Bravina, Yurii Kvasiuk, Sergey Sivoklokov, Oleksandr Vitiuk, Evgeny Zabrodin   +4 more
doaj   +1 more source

Engineering Improves Enzymatic Synthesis of L-Tryptophan by Tryptophan Synthase from Escherichia coli

Microorganisms, 2020
To improve the thermostability of tryptophan synthase, the molecular modification of tryptophan synthase was carried out by rational molecular engineering.
Lisheng Xu, Fangkai Han, Zeng Dong, Zhaojun Wei   +3 more
doaj   +1 more source