Results 81 to 90 of about 489,553 (296)

Stability-mediated epistasis constrains the evolution of an influenza protein. [PDF]

, 2013
John Maynard Smith compared protein evolution to the game where one word is converted into another a single letter at a time, with the constraint that all intermediates are words: WORD→WORE→GORE→GONE→GENE. In this analogy, epistasis constrains evolution,
Bloom, Jesse D, Gong, Lizhi Ian, Suchard, Marc A   +2 more
core   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

A C35 Carotenoid Biosynthetic Pathway [PDF]

, 2003
Upon coexpression with Erwinia geranylgeranyldiphosphate (GGDP) synthase in Escherichia coli, C30 carotenoid synthase CrtM from Staphylococcus aureus produces novel carotenoids with the asymmetrical C35 backbone.
Arnold, Frances H., Umeno, Daisuke
core  

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Engineering and In Vitro Selection of a Novel AAV3B Variant with High Hepatocyte Tropism and Reduced Seroreactivity

Molecular Therapy: Methods & Clinical Development, 2020
Limitations to successful gene therapy with adeno-associated virus (AAV) can comprise pre-existing neutralizing antibodies to the vector capsid that can block cellular entry, or inefficient transduction of target cells that can lead to sub-optimal ...
Moanaro Biswas, Damien Marsic, Ning Li, Chenhui Zou, Gloria Gonzalez-Aseguinolaza, Irene Zolotukhin, Sandeep R.P. Kumar, Jyoti Rana, John S.S. Butterfield, Oleksandr Kondratov, Ype P. de Jong, Roland W. Herzog, Sergei Zolotukhin   +12 more
doaj   +1 more source

525. Directed Molecular Evolution of Transcription Factors in Mammalian Cells for Enhanced Directed Cell Differentiation [PDF]

Molecular Therapy, 2016
The advent of cellular reprogramming has introduced new possibilities for regenerative medicine, drug discovery, and controlling cellular behavior. Recent studies have identified many natural transcription factors that are capable of directing lineage-specific differentiation in adult somatic cells.
Ami M. Kabadi, Tyler S. Klann, Malathi Chellappan, Jennifer Kwon, Timothy E. Reddy, Charles A. Gersbach   +5 more
openaire   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Molecular Bio-imprinting of Biocatalysts [PDF]

, 2011
Energy conservation is the cry of the day. Attempts are made all over the world to occupy and use energy reserves. Increased industrialization and mechanization has led to the depletion of natural energy reserves. Its unavoidable to search for renewable
Israr Khan, Muhammad Waheed Akhtar
core   +1 more source

Transverse momentum dependence of directed particle flow at 160 AGeV

, 2000
The transverse momentum ($p_t$) dependence of hadron flow at SPS energies is studied. In particular, the nucleon and pion flow in S+S and Pb+Pb collisions at 160 AGeV is investigated.
A. M. Poskanzer, A. M. Poskanzer, A. Wagner, Amand Faessler, B.-A. Li, B.-A. Li, C. Fuchs, C. M. Hung, D. H. Rischke, D. H. Rischke, E. E. Zabrodin, G. D. Yen, H. Appelshäuser, H. Heisenberg, H. Liu, H. Schlagheck, H. Sorge, H. Sorge, H. Sorge, H. Sorge, H. Sorge, H. Stöcker, J. Barrette, J. Brachmann, J. Cleymans, J. Sollfrank, J.-Y. Ollitrault, J.-Y. Ollitrault, K. A. Bugaev, K. Werner, L. P. Csernai, L. P. Csernai, L. V. Bravina, L. V. Bravina, L. V. Bravina, L. V. Bravina, L. V. Bravina, L. V. Bravina, L. V. Bravina, L.V. Bravina, L.V. Bravina, M. M. Aggarwal, N. Borghini, N. Herrmann, N. S. Amelin, N. S. Amelin, N. S. Amelin, N. S. Amelin, P. Danielewicz, P. F. Kolb, P. M. Dinh, R. J. M. Snellings, S. A. Bass, S. A. Bass, S. A. Bass, S. A. Voloshin, S. A. Voloshin, S. Nishimura, S. Voloshin, T. Gaitanos, T. Gaitanos, T. Peitzmann, V. K. Magas, V. S. Uma Maheswari, W. Reisdorf   +64 more
core   +2 more sources

Structural and dynamic changes associated with beneficial engineered single-amino-acid deletion mutations in enhanced green fluorescent protein. [PDF]

, 2014
Single-amino-acid deletions are a common part of the natural evolutionary landscape but are rarely sampled during protein engineering owing to limited and prejudiced molecular understanding of mutations that shorten the protein backbone.
Arpino, JA, Jones, DD, Rizkallah, PJ
core   +3 more sources