Results 101 to 110 of about 33,942 (277)

Fibroblast growth factor stimulates photoreceptor differentiation in vitro

, 1992
Dissociated newborn rat retinal cells were maintained in monolayer culture for periods of up to 11 d. When grown in the absence of exogenous growth factors, 1–2% of the total neuronal population expressed opsin (the photopigment that is specific for ...
D Hicks, Y Courtois
core   +1 more source

Genome‐Wide CRISPR Screen Identifies a microRNA Orchestrating Pleiotropic Resistance to Targeted Therapy and T Cell Immunity in Melanoma

Advanced Science, EarlyView.
A genome‐wide microRNA CRISPR screen identifies miR‐18a as a master regulator of cross‐resistance in melanoma. Loss of miR‐18a activates the AJUBA–YAP/Hippo axis to confer BRAFi resistance and enhances THBS1–CD47 interaction to impair CD8+ T cell immunity. hnRNP A1 is identified as an upstream regulator of miR‐18a processing.
Zhao Wang, Haotian Liu, Xueqi Wang, Jianjin Teng, Zihan Zheng, Jingya Zhang, Wanyi Xiao, Qian Liang, Jiaxin Li, Xiaomeng Jia, Xin Feng, Hongzhang Cui, Menghan Luo, Tielong Yang, Liuxing Wu, Ke Zhao, Weilong Yang, Mulin Jun Li, Dandan Huang, Jilong Yang   +19 more
wiley   +1 more source

Studies on the Role of Fibroblast Growth Factor Signaling in Neurogenesis Using Conjugated/Aged Animal Caps and Dorsal Ectoderm-Grafted Embryos

, 1997
Basic fibroblast growth factor (bFGF) has been shown to induce neural fate in dissociated animal cap (AC) cells or in AC explants cultured in low calcium and magnesium concentrations.
Ren-He Xu, Dvora Sredni, Masanori Taira, Jaebong Kim, Hsiang-fu Kung   +4 more
core   +1 more source

Mechanostimulatory Cues Determine Intestinal Fibroblast Fate and Profibrotic Remodeling in a Physiodynamic Human Gut‐on‐a‐Chip

Advanced Science, EarlyView.
A healthy gut barrier shields underlying fibroblasts from luminal shear forces, illustrating that “good fences make good neighbors.” Barrier damage exposes fibroblasts to shear stress, inducing cell death and the emergence of stress‐adapted, profibrotic fibroblasts. Sustained shear exposure promotes the formation of stiff aggregates of mechanoadapative
Soyoun Min, Nam Than, Yong Cheol Shin, Elif G. Ertugral, Chandrasekhar R. Kothapalli, Olumuyiwa Awoniyi, Hyun Jung Kim   +6 more
wiley   +1 more source

High dimensional analyses of cells dissociated from cryopreserved synovial tissue

, 2018
BackgroundDetailed molecular analyses of cells from rheumatoid arthritis (RA) synovium hold promise in identifying cellular phenotypes that drive tissue pathology and joint damage.
Edd Ricker, Peter K. Gregersen, Deepak A. Rao, Soumya Raychaudhuri, Shuqiang Li, Fumitaka Mizoguchi, Michael B. Brenner, Jason D. Turner, Vivian P. Bykerk, Ellen M. Gravallese, Andrew Filer, David J. Lieb, David L. Boyle, Stephen Kelly, Nir Hacohen, Akiko Noma, Jennifer H. Anolik, Cristina Rozo, Susan M. Goodman, Nida Meednu, Chad Nusbaum, Paul J. Utz, James A. Lederer, Joshua Hillman, Laura T. Donlin, Kaylin Muskat, Gary S. Firestein, V. Michael Holers, Maria Gutierrez-Arcelus, Thomas M. Eisenhaure, Alessandra B. Pernis, Larry W. Moreland, Adam Chicoine, Joshua Keegan, Kevin Wei, Edward P. Browne, Lionel B. Ivashkiv, null null, William H. Robinson, Brendan F. Boyce, Kamil Slowikowski, Mandy J. McGeachy, Danielle Sutherby, Costantino Pitzalis   +43 more
core   +1 more source

Targeting the PDK1/c‐Myc/SOX10 Signaling in Oligodendrocytes Alleviates Neuropathic Pain

Advanced Science, EarlyView.
This work reveals that oligodendrocyte homeostasis, mediated by PDK1, is a critical determinant of neuropathic pain (NPP) pathogenesis. Disruption of PDK1 in oligodendrocytes impairs SOX10‐dependent myelination programs through c‐Myc accumulation, leading to disrupted myelination and the pathophysiology of NPP.
Pingping Qiao, Lifang Guo, Guochao Yang, Chaoli Huang, He Wang, Jian‐Jun Yang, Guiquan Chen, Yimin Hu   +7 more
wiley   +1 more source

Additional file 6: of Renal cancer: new models and approach for personalizing therapy

, 2018
Figure S5. Freshly dissociated tissues were maintained three days in serum-free stem cell-isolating medium supplemented with Epidermal Growth Factor (EGF) and basic Fibroblast Growth Factor (b-FGF).
Michele Milella (725941), Massimo Sanchez (183600), Michele Signore (129959), Steno Sentinelli (5722820), Laura De Salvo (3740305), Antonio Addario (5722811), Valentina Tirelli (547526), Isabella Sperduti (85365), Ruggero De Maria (129963), Romina Alfonsi (1549981), Giovanni Muto (375271), Manuela Costantini (5722823), Ludovica Grassi (5722796), Giulia Federici (5722805), Gabriele De Luca (244081), Luca Pasquini (168695), Giuseppe Simone (5722829), DĂŠsirĂŠe Bonci (5722838), Simona di Martino (5722790), Federica Francescangeli (5722814), Mustapha Haoui (5722826), Michele Gallucci (5722835)   +21 more
core   +1 more source

Activation of ERBB4 Pathway Inhibits Pathological Transdifferentiation of Lung Epithelial Progenitors into CD66c+ Basal Cells in Severe Lung Injury

Advanced Science, EarlyView.
In fibrotic distal lung regions, CD66c+ basal cells emerge as a pathological state. Using human distal lung organoids, this study identifies CD66c+ basal cells as a pro‐fibrotic state arising through transdifferentiation from secretory, AT2, and basal cells.
Kaijun Lin, Xinran Deng, Haonan Wang, Yamei Jiang, Jian Sun, Hailin Ding, Ming Ye, Xiaoting Wang, Yu Wang, Li Yuan, Zhenju Song, Xinhua Lin, Shenfei Sun, Ning Jiang   +13 more
wiley   +1 more source

Guest-responsive, Non-proteolytic Harvest of a Cell-sheet using Controllable Host-guest Chemistry

, 2017
A new non-proteolytic method to harvest a cell-sheet was demonstrated using controllable host-guest interactions which can be dissociated by treating a strong guest on demand.
Park, Kyeng Min, Kim, Kimoon, Annadka Shrinidhi, Kyeng Min Park, James Murray, Shrinidhi, Annadka, Murray, James, Kimoon Kim   +7 more
core   +1 more source

Mitochondria and Neuromast Tagging With Fluorescent Gallium‐Triapine Analogues: In Cellulo MP FLIM and Zebrafish Live Imaging

Advanced Science, EarlyView.
Fluorescent BODIPY‐conjugated thiosemicarbazone ligands and their Ga(III), In(III), and Fe(III) complexes, inspired by Triapine, are developed as theranostic agents. Multiphoton FLIM and confocal microscopy in cancer cells and zebrafish reveal real‐time uptake, mitochondrial localisation, and whilst spectroscopic assays indicated preserved complex ...
Megan J. Green, Michael W. Jones, Merissa Saleem, Haobo Ge, Melita Tvardauskaite, Julia Dudzic, Fernando Cortezon‐Tamarit, Rory L. Arrowsmith, Charareh Pourzand, Gabriele Kociock‐Kohn, Nicholas H. Rees, Jonathan R. Dilworth, Stephen Faulkner, Stanley W. Botchway, David Gurevich, Sofia I. Pascu   +15 more
wiley   +1 more source