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Diversity of Fungal DNA Methyltransferases and Their Association With DNA Methylation Patterns [PDF]

open access: yesFrontiers in Microbiology, 2021
DNA methyltransferases (DNMTs) are a group of proteins that catalyze DNA methylation by transferring a methyl group to DNA. The genetic variation in DNMTs results in differential DNA methylation patterns associated with various biological processes.
Yu-Shin Nai   +6 more
doaj   +3 more sources

Optimization of DNA extraction from human urinary samples for mycobiome community profiling. [PDF]

open access: yesPLoS ONE, 2019
IntroductionRecent data suggest the urinary tract hosts a microbial community of varying composition, even in the absence of infection. Culture-independent methodologies, such as next-generation sequencing of conserved ribosomal DNA sequences, provide an
A Lenore Ackerman   +8 more
doaj   +4 more sources

Comparison of methods for isolating fungal DNA

open access: yesPractical Laboratory Medicine, 2021
Objectives: The main aim of this work was to compare the methods of DNA isolation in the moulds of genus Mucorales with special regard to the amount and purity of the DNA acquired. The acquired DNA was then amplified by specific real-time PCR.
Ilaria Galliano   +7 more
doaj   +4 more sources

Uncovering unseen fungal diversity from plant DNA banks [PDF]

open access: yesPeerJ, 2017
Throughout the world DNA banks are used as storage repositories for genetic diversity of organisms ranging from plants to insects to mammals. Designed to preserve the genetic information for organisms of interest, these banks also indirectly preserve ...
Erin M. Datlof   +7 more
doaj   +4 more sources

Dye-based qPCR assays for the separate quantification of total bacterial, fungal, arthropod, and plant DNA. [PDF]

open access: yesPLoS ONE
Characterization of bacterial, fungal, arthropod, and plant communities from bulk environmental samples can enhance our understanding of the biodiversity on Earth and aid in biomonitoring of various biomes.
Teresa M Tiedge   +2 more
doaj   +2 more sources

Comparison of methods for isolating fungal DNA [PDF]

open access: yesCzech Journal of Food Sciences, 2011
In this study methods of fungal DNA isolation were optimised and compared. The aim of the isolation processes was to obtain DNA of sufficient quality and quantity necessary for its amplification, as most detection techniques require DNA amplification ...
Petra MOŤKOVÁ, Jarmila VYTŘASOVÁ
doaj   +2 more sources

Influence of DNA extraction kits on freshwater fungal DNA metabarcoding [PDF]

open access: yesPeerJ, 2021
AbstractBackgroundEnvironmental DNA (eDNA) metabarcoding is a common technique for efficient biodiversity monitoring, especially of microbes. Recently, the usefulness of aquatic eDNA in monitoring the diversity of both terrestrial and aquatic fungi has been suggested.
Shunsuke Matsuoka   +3 more
openaire   +3 more sources

Epigenetic Regulation of Antifungal Drug Resistance

open access: yesJournal of Fungi, 2022
In medical mycology, epigenetic mechanisms are emerging as key regulators of multiple aspects of fungal biology ranging from development, phenotypic and morphological plasticity to antifungal drug resistance.
Sandip Patra   +3 more
doaj   +1 more source

An Assay to Determine NAD(P)H: Quinone Oxidoreductase Activity in Cell Extracts from Candida glabrata

open access: yesBio-Protocol, 2021
Flavodoxin-like proteins (Fld-LPs) are an important constituent of the oxidative stress defense system in several organisms and highly conserved from bacteria to humans.
Anamika Battu   +2 more
doaj   +1 more source

Phosphatidylinositol 3-phosphate regulates iron transport via PI3P-binding CgPil1 protein

open access: yesCell Reports, 2023
Summary: Iron homeostasis, which is pivotal to virulence, is regulated by the phosphatidylinositol 3-kinase CgVps34 in the human fungal pathogen Candida glabrata.
Fizza Askari   +2 more
doaj   +1 more source

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