Results 1 to 10 of about 323,377 (241)
Influence of DNA extraction kits on freshwater fungal DNA metabarcoding [PDF]
PeerJ, 2021 AbstractBackgroundEnvironmental DNA (eDNA) metabarcoding is a common technique for efficient biodiversity monitoring, especially of microbes. Recently, the usefulness of aquatic eDNA in monitoring the diversity of both terrestrial and aquatic fungi has been suggested.
Shunsuke Matsuoka +3 more
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Diversity of Fungal DNA Methyltransferases and Their Association With DNA Methylation Patterns [PDF]
Frontiers in Microbiology, 2021 DNA methyltransferases (DNMTs) are a group of proteins that catalyze DNA methylation by transferring a methyl group to DNA. The genetic variation in DNMTs results in differential DNA methylation patterns associated with various biological processes.
Yu-Shin Nai +6 more
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Comparison of methods for isolating fungal DNA
Practical Laboratory Medicine, 2021 The main aim of this work was to compare the methods of DNA isolation in the moulds of genus Mucorales with special regard to the amount and purity of the DNA acquired. The acquired DNA was then amplified by specific real-time PCR.Five DNA extraction procedures were carried out in a Class 2 Biosafety cabinet in a dedicated room with suitable biosafety ...
Galliano I. +7 more
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Mycobiome of the Bat White Nose Syndrome (WNS) Affected Caves and Mines reveals High Diversity of Fungi and Local Adaptation by the Fungal Pathogen Pseudogymnoascus (Geomyces) destructans [PDF]
, 2014 The investigations of the bat White Nose Syndrome (WNS) have yet to provide answers as to how the causative fungus Pseudogymnoascus (Geomyces) destructans (Pd) first appeared in the Northeast and how a single clone has spread rapidly in the US and Canada.
Broussard, Kelly +10 more
core +7 more sources
The use of flow cytometry for fungal nuclear DNA quantification [PDF]
Cytometry Part A, 2021 AbstractGenome size information is sparse across fungi, with information being available for less than 2000 species. So far, most records have been obtained using static, microscope‐based cytometry methods or derived from genome sequencing projects. Flow cytometry is now considered the state‐of‐the‐art method for obtaining genome size measurements, and
Pedro Talhinhas +2 more
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Comparison of methods for isolating fungal DNA [PDF]
Czech Journal of Food Sciences, 2011 In this study methods of fungal DNA isolation were optimised and compared. The aim of the isolation processes was to obtain DNA of sufficient quality and quantity necessary for its amplification, as most detection techniques require DNA amplification before the proper DNA detection itself.
Jarmila Vytřasová, Petra Moťková
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The Quest for a General and Reliable Fungal DNA Barcode [PDF]
The Open Applied Informatics Journal, 2011 DNA sequences are key elements for both identification and classification of living organisms. Mainly for historical reasons, a limited number of genes are currently used for this purpose. From a mathematical point of view, any DNA segment, at any location, even outside of coding regions and even if they do not align, could be used as long as PCR ...
V. Robert +7 more
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Fungal CTAB DNA Extraction v1 [PDF]
, 2020 This protocol is a CTAB DNA extraction method for filamentous fungi. Its purpose is to extract high molecular weight genomic DNA for genome sequencing.
Derreck Carter-House +3 more
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Use of magnetic beads to extract fungal DNA [PDF]
Mycoses, 2005 SummaryAuthors compare two methods of extracting DNA from different fungi: the classic method with phenol/chloroform (P/C) and that with magnetic beads. Both were tested on Candida albicans and Cryptococcus neoformans var. neoformans, belonging to the yeast group and Microsporum canis, M. gypseum, Trichophyton rubrum, T. interdigitale, T.
FAGGI, ELISABETTA +2 more
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Optimization of DNA extraction from human urinary samples for mycobiome community profiling. [PDF]
, 2019 IntroductionRecent data suggest the urinary tract hosts a microbial community of varying composition, even in the absence of infection. Culture-independent methodologies, such as next-generation sequencing of conserved ribosomal DNA sequences, provide an
Ackerman, A Lenore +8 more
core +3 more sources