The Pattern and Function of DNA Methylation in Fungal Plant Pathogens [PDF]
To successfully infect plants and trigger disease, fungal plant pathogens use various strategies that are dependent on characteristics of their biology and genomes. Although pathogenic fungi are different from animals and plants in the genomic heritability, sequence feature, and epigenetic modification, an increasing number of phytopathogenic fungi ...
Chang He+3 more
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Assessing the effect of organic residue quality on active decomposing fungi in a tropical Vertisol using 15N-DNA stable isotope probing [PDF]
15N-DNA stable isotope probing (15N-DNA-SIP) combined with 18S rRNA gene-based community analysis was used to identify active fungi involved in decomposition of 15N-labeled maize and soybean litter in a tropical Vertisol.
Belkys Rodriguez+34 more
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Sampling Local Fungal Diversity in an Undergraduate Laboratory using DNA Barcoding [PDF]
Traditional methods for fungal species identification require diagnostic morphological characters and are often limited by the availability of fresh fruiting bodies and local identification resources.
Anderson, B. W.+18 more
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Rapid Mini-Preparation of Fungal DNA for PCR [PDF]
Nucleic acid detection methods such as PCR have become a common tool for microbial identification and diagnosis. Although PCR amplification can be performed directly for various microbial cultures, for filamentous fungi and yeasts, prior isolation of DNA is often preferred.
S. Coloe+3 more
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An efficient method for DNA extraction from Cladosporioid fungi [PDF]
We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Abd-Elsalam, K.A.+3 more
core +2 more sources
Nucleic acids enrichment of fungal pathogens to study host-pathogen interactions [PDF]
Fungal infections, ranging from superficial to life-threatening infections, represent a major public health problem that affects 25% of the worldwide population.
Coucke, Paul+3 more
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Identification of Contaminating Fungal DNA Sequences in Zymolyase [PDF]
ABSTRACT When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu ...
Dagmar Rimek+3 more
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Impact of tillage system on arbuscular mycorrhiza fungal communities in the soil under Mediterranean conditions. [PDF]
A more diverse arbuscular mycorrhiza (AM) fungal community should be more versatile and resilient to variation in environmental conditions over space and time.
Brito, Isabel+4 more
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Fungal DNA Extraction from Fungal Plate v1
This protocol describes how to do fungal DNA extraction from fungal plate. This protocol is part of the Bark Beetle Mycobiome (BBM) Research Coordination Network. For more information on the BBM international network: Hulcr J, Barnes I, De Beer ZW, Duong TA, Gazis R, Johnson AJ, Jusino MA, Kasson MT, Li Y, Lynch S, Mayers C, Musvuugwa T, Roets F ...
You Li, Demian F Gomez
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A simple method for extraction of fungal genomic DNA [PDF]
We have developed a new, simple and effective method for extraction of fungal genomic DNA. The initial steps involved suspension of freeze-dried mycelium in buffer containing sodium dodecyl sulphate, detachment of DNA from polysaccharides by mild shearing, NaCl precipitation of polysaccharides and protein, chloroform extraction and ethanol ...
Taha H. Al-Samarrai, Jan Schmid
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