Results 11 to 20 of about 363,056 (280)

Comparison of methods for isolating fungal DNA

open access: yesPractical Laboratory Medicine, 2021
Objectives: The main aim of this work was to compare the methods of DNA isolation in the moulds of genus Mucorales with special regard to the amount and purity of the DNA acquired. The acquired DNA was then amplified by specific real-time PCR.
Ilaria Galliano   +7 more
doaj   +4 more sources

Uncovering unseen fungal diversity from plant DNA banks [PDF]

open access: yesPeerJ, 2017
Throughout the world DNA banks are used as storage repositories for genetic diversity of organisms ranging from plants to insects to mammals. Designed to preserve the genetic information for organisms of interest, these banks also indirectly preserve ...
Erin M. Datlof   +7 more
doaj   +4 more sources

Comparison of methods for isolating fungal DNA [PDF]

open access: yesCzech Journal of Food Sciences, 2011
In this study methods of fungal DNA isolation were optimised and compared. The aim of the isolation processes was to obtain DNA of sufficient quality and quantity necessary for its amplification, as most detection techniques require DNA amplification ...
Petra MOŤKOVÁ, Jarmila VYTŘASOVÁ
doaj   +2 more sources

Influence of DNA extraction kits on freshwater fungal DNA metabarcoding [PDF]

open access: yesPeerJ, 2021
AbstractBackgroundEnvironmental DNA (eDNA) metabarcoding is a common technique for efficient biodiversity monitoring, especially of microbes. Recently, the usefulness of aquatic eDNA in monitoring the diversity of both terrestrial and aquatic fungi has been suggested.
Shunsuke Matsuoka   +3 more
openaire   +3 more sources

Fungal CTAB DNA Extraction v1 [PDF]

open access: yes, 2020
This protocol is a CTAB DNA extraction method for filamentous fungi. Its purpose is to extract high molecular weight genomic DNA for genome sequencing.
Derreck Carter-House   +3 more
openaire   +1 more source

Candida glabrata: A Lot More Than Meets the Eye

open access: yesMicroorganisms, 2019
Candida glabrata is an opportunistic human fungal pathogen that causes superficial mucosal and life-threatening bloodstream infections in individuals with a compromised immune system. Evolutionarily, it is closer to the non-pathogenic yeast Saccharomyces
Kundan Kumar   +3 more
doaj   +1 more source

An efficient method for DNA extraction from Cladosporioid fungi [PDF]

open access: yes, 2010
We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Abd-Elsalam, K.A.   +3 more
core   +2 more sources

Mycelial DNA persistence in a forest soil

open access: yesEnvironmental DNA, 2021
Living fungi can be identified and monitored in their soil habitat using an array of molecular methods. However, DNA originating from dead fungal tissue may become stabilized in the soil and remain detectable for some time after cell death rendering ...
Matthew Gordon, Kelli Van Norman
doaj   +1 more source

Evolutionary Relationship and the Sequence Similarities among Different Fungal Species Infecting Birds Captured from Different Areas in Denmark [PDF]

open access: yesArchives of Razi Institute, 2022
Fungal diseases are the common cause of death in wild animals and birds of prey. This study was designed to investigate the development of fungal infections among wild birds in Denmark.
Z Shaker Al-Rubaiee   +2 more
doaj   +1 more source

Rapid Mini-Preparation of Fungal DNA for PCR [PDF]

open access: yesJournal of Clinical Microbiology, 2000
Nucleic acid detection methods such as PCR have become a common tool for microbial identification and diagnosis. Although PCR amplification can be performed directly for various microbial cultures, for filamentous fungi and yeasts, prior isolation of DNA is often preferred.
D, Liu, S, Coloe, R, Baird, J, Pederson
openaire   +2 more sources

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