Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi [PDF]
, 2019 Background: Within the last years, numerous reports described successful application of the CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi.
Haefner, Stefan +4 more
core +1 more source
General Fungal DNA Extraction v2
, 2023 This is a routine protocol for extracting DNA from various fungi. This extraction method is suitable for follow-up molecular work such as PCR amplification.
Angie Macias +2 more
openaire +1 more source
mBio, 2021 Although relatively few in number, studies of DNA methylation have shown that fungal DNA methylation is implicated in development, genome integrity, and genome defense.
Yo-Han Ko +3 more
doaj +1 more source
An RNAi-Based Control of Fusarium graminearum Infections Through Spraying of Long dsRNAs Involves a Plant Passage and Is Controlled by the Fungal Silencing Machinery [PDF]
, 2016 Meeting the increasing food and energy demands of a growing population will require the development of ground-breaking strategies that promote sustainable plant production.
Abdellatef, Eltayb +13 more
core +3 more sources
Using DNA Barcoding in Fungal Taxonomy
Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 2020 Totally, 1.5 million fungal species estimated, of which less than 10 percent have been described to date. Recent advances in molecular biology as well as gene sequencing technologies facilitate the discovery and identification of new species in various groups of living organisms, especially fungi.
Younes Rezaee Danesh, Semra Demir
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Tracking fungal community responses to maize plants by DNA- and RNA-based pyrosequencing. [PDF]
PLoS ONE, 2013 We assessed soil fungal diversity and community structure at two sampling times (t1 = 47 days and t2 = 104 days of plant age) in pots associated with four maize cultivars, including two genetically modified (GM) cultivars by high-throughput ...
Eiko E Kuramae +6 more
doaj +1 more source
Aspergillus westerdijkiae polyketide synthase gene “aoks1” is involved in the biosynthesis of ochratoxin A [PDF]
, 2009 OchratoxinA (OTA) is a potential nephrotoxic, teratogenic, immunogenic, hepatotoxic and carcinogenic mycotoxin, produced by Aspergillus westerdijkiae NRRL 3174.
Atoui, Ali +4 more
core +2 more sources
Identification of Contaminating Fungal DNA Sequences in Zymolyase [PDF]
Journal of Clinical Microbiology, 1999 ABSTRACT When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu ...
D, Rimek, A P, Garg, W H, Haas, R, Kappe
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DNA quantification of basidiomycetous fungi during storage of logging residues [PDF]
PeerJ, 2015 The demand for bioenergy caused an increased use of logging residues, branches and treetops that were previously left on the ground after harvesting. Residues are stored outdoors in piles and it is unclear to what extent fungi transform this material ...
Isabella Børja +3 more
doaj +2 more sources
Nucleic acids enrichment of fungal pathogens to study host-pathogen interactions [PDF]
, 2019 Fungal infections, ranging from superficial to life-threatening infections, represent a major public health problem that affects 25% of the worldwide population.
Coucke, Paul +3 more
core +1 more source