PCNA and XPF cooperate to distort DNA substrates [PDF]
, 2009 XPF is a structure-specific endonuclease that preferentially cleaves 3' DNA flaps during a variety of repair processes. The crystal structure of a crenarchaeal XPF protein bound to a DNA duplex yielded insights into how XPF might recognise branched DNA ...
J. Carlos Penedo +12 more
core +1 more source
Coordinated Binding of Single-Stranded and Double-Stranded DNA by UvsX Recombinase. [PDF]
PLoS ONE, 2013 Homologous recombination is important for the error-free repair of DNA double-strand breaks and for replication fork restart. Recombinases of the RecA/Rad51 family perform the central catalytic role in this process.
Robyn L Maher, Scott W Morrical
doaj +1 more source
The helicase XPD unwinds bubble structures and is not stalled by DNA lesions removed by the nucleotide excision repair pathway [PDF]
, 2010 Xeroderma pigmentosum factor D (XPD) is a 5'-3' superfamily 2 helicase and the founding member of a family of DNA helicases with iron-sulphur cluster domains.
Rouillon, Christophe +3 more
core +1 more source
Towards quantitative viromics for both double-stranded and single-stranded DNA viruses [PDF]
PeerJ, 2016 Background Viruses strongly influence microbial population dynamics and ecosystem functions. However, our ability to quantitatively evaluate those viral impacts is limited to the few cultivated viruses and double-stranded DNA (dsDNA) viral genomes ...
Simon Roux +8 more
doaj +2 more sources
Experimental test of connector rotation during DNA packaging into bacteriophage phi29 capsids [PDF]
, 2007 The bacteriophage phi29 generates large forces to compact its double-stranded DNA genome into a protein capsid by means of a portal motor complex. Several mechanical models for the generation of these high forces by the motor complex predict coupling of ...
Hetherington, Craig L. +26 more
core +1 more source
BMC Genomics, 2019 Background Cell-free DNA (cfDNA), present in circulating blood plasma, contains information about prenatal health, organ transplant reception, and cancer presence and progression.
Christopher J. Troll +8 more
doaj +1 more source
Two time constants for the binding of proteins to DNA from micromechanical data [PDF]
, 2000 Recent experimental advances allow the direct measurement of the force/extension behavior for DNA in the presence of strongly binding proteins. Such experiments reveal information about the cooperative mechanism of protein binding.
Turner, Matthew S., Matthew S. Turner
core +1 more source
Folding of single‐stranded DNA on the histone octamer [PDF]
FEBS Letters, 1985 A complex between the single‐stranded DNA of the bacteriophage M13 and the histone octamer was analyzed by electron microscopy, low‐angle X‐ray diffraction and nuclease analysis. The morphology and the diffraction pattern of the complex strongly resemble those of the nucleosome.
Caffarelli E, Leoni L, Savino M
openaire +3 more sources
Insertional Mutagenesis by CRISPR/Cas9 Ribonucleoprotein Gene Editing in Cells Targeted for Point Mutation Repair Directed by Short Single-Stranded DNA Oligonucleotides. [PDF]
PLoS ONE, 2017 CRISPR/Cas9 and single-stranded DNA oligonucleotides (ssODNs) have been used to direct the repair of a single base mutation in human genes. Here, we examine a method designed to increase the precision of RNA guided genome editing in human cells by ...
Natalia Rivera-Torres +4 more
doaj +1 more source
Purification of single-stranded DNA by co-polymerization with acrylamide and electrophoresis
BioTechniques, 2017 Single-stranded DNA (ssDNA) oligonucleotides are useful as aptamers, hybridization probes and for emerging applications in DNA nanotechnology. Current methods to purify ssDNA require both a strand-separation step and a separate size-separation step but ...
Tulsi Ram Damase +2 more
doaj +1 more source